Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3344 and PP_3345 are separated by 0 nucleotides PP_3345 and PP_3346 overlap by 4 nucleotides PP_3346 and PP_3348 are separated by 307 nucleotides
PP_3344: PP_3344 - nickel ABC transporter permease subunit, at 3,782,064 to 3,782,909
_3344
PP_3345: PP_3345 - nickel ABC transporter ATP-binding subunit, at 3,782,910 to 3,783,680
_3345
PP_3346: PP_3346 - Nickel transporter ATP-binding protein, at 3,783,677 to 3,784,498
_3346
PP_3348: PP_3348 - GGDEF domain protein, at 3,784,806 to 3,785,951
_3348
Position (kb)
3783
3784
3785 Strain fitness (log2 ratio)
-2
-1
0
1 at 3783.073 kb on + strand, within PP_3345 at 3783.718 kb on + strand at 3783.718 kb on + strand at 3783.757 kb on - strand at 3784.325 kb on + strand, within PP_3346 at 3784.326 kb on - strand, within PP_3346 at 3784.536 kb on + strand at 3784.536 kb on + strand at 3784.537 kb on - strand at 3784.537 kb on - strand at 3784.537 kb on - strand at 3784.537 kb on - strand at 3784.537 kb on - strand at 3784.842 kb on + strand at 3784.842 kb on + strand at 3784.843 kb on - strand at 3784.843 kb on - strand at 3784.843 kb on - strand at 3784.846 kb on + strand at 3784.846 kb on + strand at 3784.846 kb on + strand at 3785.025 kb on + strand, within PP_3348 at 3785.211 kb on + strand, within PP_3348 at 3785.211 kb on + strand, within PP_3348 at 3785.212 kb on - strand, within PP_3348 at 3785.212 kb on - strand, within PP_3348
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,783,073 + PP_3345 0.21 -0.9 3,783,718 + +1.2 3,783,718 + +0.8 3,783,757 - +0.1 3,784,325 + PP_3346 0.79 -1.8 3,784,326 - PP_3346 0.79 -1.5 3,784,536 + +0.6 3,784,536 + -1.5 3,784,537 - -0.2 3,784,537 - +0.3 3,784,537 - +0.2 3,784,537 - -0.2 3,784,537 - +0.8 3,784,842 + -0.4 3,784,842 + -0.3 3,784,843 - -0.3 3,784,843 - -1.3 3,784,843 - +1.5 3,784,846 + +0.5 3,784,846 + -0.1 3,784,846 + -1.1 3,785,025 + PP_3348 0.19 -0.3 3,785,211 + PP_3348 0.35 +0.1 3,785,211 + PP_3348 0.35 -0.4 3,785,212 - PP_3348 0.35 +0.7 3,785,212 - PP_3348 0.35 +0.4
Or see this region's nucleotide sequence