Strain Fitness in Pseudomonas putida KT2440 around PP_3285

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
3,718,844	-		PP_3284	0.28	+0.6
3,718,960	+		PP_3284	0.43	-2.7
3,718,960	+		PP_3284	0.43	-1.0
3,718,961	-		PP_3284	0.43	+1.1
3,718,961	-		PP_3284	0.43	-0.0
3,719,149	+		PP_3284	0.67	+1.5
3,719,305	+		PP_3284	0.88	-1.7
3,719,388	+				-1.6
3,719,388	+				+0.8
3,719,388	+				+0.1
3,719,388	+				+0.9
3,719,389	-				-0.7
3,719,390	+				+0.3
3,719,391	-				-0.8
3,719,472	-				+0.1
3,719,472	-				-1.8
3,719,472	-				-0.8
3,719,503	+				-2.2
3,719,503	+				+0.3
3,719,504	-				+1.6
3,719,569	-				+1.8
3,719,632	+				-1.1
3,719,633	-				-1.2
3,719,676	-				+0.2
3,719,798	+				+0.1
3,719,798	+				+0.8
3,719,798	+				+2.1
3,719,798	+				-0.4
3,719,799	-				-0.5
3,719,799	-				-0.5
3,719,852	+		PP_3285	0.17	-1.1
3,719,853	-		PP_3285	0.18	-1.1
3,719,974	+		PP_3285	0.38	-1.5
3,720,072	+		PP_3285	0.54	-2.0
3,720,072	+		PP_3285	0.54	-0.4
3,720,072	+		PP_3285	0.54	+1.4
3,720,073	-		PP_3285	0.54	-0.1
3,720,073	-		PP_3285	0.54	-1.8
3,720,073	-		PP_3285	0.54	+1.4
3,720,073	-		PP_3285	0.54	-0.1
3,720,222	-		PP_3285	0.79	-1.6
3,720,390	+				+0.8
3,720,390	+				-0.0
3,720,390	+				-0.5
3,720,390	+				+1.6
3,720,391	-				+0.1
3,720,391	-				-0.5
3,720,391	-				+0.8
3,720,391	-				-1.3
3,720,392	+				+0.3
3,720,392	+				-0.4
3,720,393	-				-0.2
3,720,393	-				-1.7
3,720,393	-				+1.8
3,720,393	-				+0.2
3,720,511	+				-0.7
3,720,512	-				+0.3
3,720,556	-		PP_3286	0.14	-0.0
3,720,603	-		PP_3286	0.19	-1.4
3,720,603	-		PP_3286	0.19	+0.5
3,720,672	+		PP_3286	0.27	+0.4
3,720,673	+		PP_3286	0.27	-0.8
3,720,673	+		PP_3286	0.27	+0.4
3,720,673	+		PP_3286	0.27	+0.2
3,720,674	-		PP_3286	0.27	+1.3
3,720,727	+		PP_3286	0.33	-0.8
3,720,727	+		PP_3286	0.33	+0.1
3,720,729	+		PP_3286	0.33	+0.1
3,720,822	-		PP_3286	0.43	-0.6
3,720,930	+		PP_3286	0.55	-0.5
3,720,959	+		PP_3286	0.58	-0.5
3,721,028	-		PP_3286	0.65	+1.1
3,721,028	-		PP_3286	0.65	+1.3
3,721,038	+		PP_3286	0.66	-0.5
3,721,039	-		PP_3286	0.67	-0.6
3,721,039	-		PP_3286	0.67	+0.9