Strain Fitness in Pseudomonas putida KT2440 around PP_3249

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
3,681,802	+				-0.1
3,681,803	-				+1.0
3,681,821	+				+0.7
3,681,822	-				-1.7
3,681,836	-				-1.4
3,681,836	-				+0.5
3,681,981	+		PP_3248	0.17	+0.5
3,681,982	-		PP_3248	0.17	+2.3
3,682,018	-		PP_3248	0.21	+0.7
3,682,018	-		PP_3248	0.21	+1.4
3,682,331	+		PP_3248	0.57	+1.1
3,682,331	+		PP_3248	0.57	+0.8
3,682,331	+		PP_3248	0.57	+0.1
3,682,332	-		PP_3248	0.57	+0.4
3,682,332	-		PP_3248	0.57	-0.4
3,682,637	+				-1.3
3,682,637	+				-0.2
3,682,641	-				-2.9
3,682,677	+				-1.7
3,682,677	+				+0.5
3,682,677	+				+0.6
3,682,678	-				+0.1
3,682,678	-				+1.0
3,682,716	+				+0.9
3,682,792	+				-0.4
3,682,792	+				-1.8
3,682,792	+				-2.0
3,682,792	+				+0.2
3,682,792	+				-0.0
3,682,792	+				+0.5
3,682,792	+				+0.4
3,682,792	+				+1.3
3,682,792	+				-1.7
3,682,793	-				-0.5
3,682,793	-				+0.9
3,682,793	-				+0.2
3,682,793	-				-1.8
3,682,793	-				+0.4
3,682,793	-				-0.4
3,682,812	-				+0.0
3,682,978	+		PP_3249	0.21	-0.2
3,682,978	+		PP_3249	0.21	+1.4
3,683,050	+				+1.6
3,683,050	+		PP_3249	0.29	-1.5
3,683,051	-		PP_3249	0.29	-0.6
3,683,051	-		PP_3249	0.29	+2.5
3,683,287	+		PP_3249	0.55	+0.0
3,683,287	+		PP_3249	0.55	+0.6
3,683,288	-		PP_3249	0.55	+1.1
3,683,288	-				+2.7
3,683,288	-		PP_3249	0.55	+0.7
3,683,305	+		PP_3249	0.57	-0.3
3,683,305	+		PP_3249	0.57	-0.4
3,683,305	+		PP_3249	0.57	+0.6
3,683,377	+		PP_3249	0.64	+0.7
3,683,377	+		PP_3249	0.64	+0.9
3,683,378	-		PP_3249	0.65	+0.7
3,683,378	-		PP_3249	0.65	-2.0
3,683,378	-		PP_3249	0.65	+0.3
3,683,602	-		PP_3249	0.89	+3.6
3,683,792	+				+0.6
3,683,792	+				-1.9
3,683,792	+				+0.2
3,683,793	-				+0.4
3,683,811	-				-0.4
3,683,811	-				+0.4
3,683,957	-		PP_3250	0.20	+0.0
3,683,972	+		PP_3250	0.21	+0.9
3,683,972	+		PP_3250	0.21	-0.3
3,683,973	-		PP_3250	0.21	+0.2
3,684,056	+		PP_3250	0.27	+1.3
3,684,056	+		PP_3250	0.27	+0.6
3,684,057	-		PP_3250	0.27	-0.3
3,684,057	-		PP_3250	0.27	+1.4
3,684,057	-		PP_3250	0.27	-1.3
3,684,057	-		PP_3250	0.27	+0.9
3,684,058	+		PP_3250	0.27	-1.2
3,684,059	-		PP_3250	0.28	-0.1
3,684,059	-		PP_3250	0.28	-0.4
3,684,250	-		PP_3250	0.42	-0.3
3,684,274	+		PP_3250	0.44	-0.1
3,684,390	+		PP_3250	0.53	-1.5
3,684,411	+		PP_3250	0.55	+0.1
3,684,462	-		PP_3250	0.59	-0.2
3,684,478	+		PP_3250	0.60	+0.1
3,684,478	+		PP_3250	0.60	+0.1
3,684,479	-		PP_3250	0.60	-1.2
3,684,479	-		PP_3250	0.60	+0.7
3,684,639	+		PP_3250	0.73	-0.1