Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3226 and PP_3227 are separated by 185 nucleotides PP_3227 and PP_3228 are separated by 201 nucleotides
PP_3226: PP_3226 - Acyl-CoA dehydrogenase-related protein, at 3,661,691 to 3,662,833
_3226
PP_3227: PP_3227 - Transcriptional regulator, LysR family, at 3,663,019 to 3,663,909
_3227
PP_3228: PP_3228 - putative Periplasmic aliphatic sulfonate-binding protein, at 3,664,111 to 3,665,076
_3228
Position (kb)
3663
3664 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3662.088 kb on + strand, within PP_3226 at 3662.089 kb on - strand, within PP_3226 at 3662.089 kb on - strand, within PP_3226 at 3662.091 kb on - strand, within PP_3226 at 3662.121 kb on - strand, within PP_3226 at 3662.343 kb on + strand, within PP_3226 at 3662.373 kb on + strand, within PP_3226 at 3662.577 kb on + strand, within PP_3226 at 3662.578 kb on - strand, within PP_3226 at 3662.685 kb on - strand, within PP_3226 at 3662.856 kb on + strand at 3662.901 kb on + strand at 3663.087 kb on - strand at 3663.136 kb on - strand, within PP_3227 at 3663.142 kb on + strand, within PP_3227 at 3663.143 kb on - strand, within PP_3227 at 3663.143 kb on - strand, within PP_3227 at 3663.176 kb on - strand at 3663.176 kb on - strand, within PP_3227 at 3663.351 kb on - strand at 3663.368 kb on + strand, within PP_3227 at 3663.894 kb on + strand at 3663.971 kb on + strand at 3663.992 kb on - strand at 3664.215 kb on + strand, within PP_3228 at 3664.264 kb on + strand at 3664.423 kb on + strand, within PP_3228 at 3664.789 kb on + strand, within PP_3228 at 3664.790 kb on - strand, within PP_3228 at 3664.839 kb on + strand, within PP_3228 at 3664.844 kb on - strand, within PP_3228
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,662,088 + PP_3226 0.35 -2.4 3,662,089 - PP_3226 0.35 -2.1 3,662,089 - PP_3226 0.35 -0.8 3,662,091 - PP_3226 0.35 +0.5 3,662,121 - PP_3226 0.38 -1.3 3,662,343 + PP_3226 0.57 +0.3 3,662,373 + PP_3226 0.60 +2.6 3,662,577 + PP_3226 0.78 +0.5 3,662,578 - PP_3226 0.78 +0.6 3,662,685 - PP_3226 0.87 +0.6 3,662,856 + -0.2 3,662,901 + +0.8 3,663,087 - -1.6 3,663,136 - PP_3227 0.13 -2.0 3,663,142 + PP_3227 0.14 -1.4 3,663,143 - PP_3227 0.14 -2.0 3,663,143 - PP_3227 0.14 +0.2 3,663,176 - +0.3 3,663,176 - PP_3227 0.18 -0.7 3,663,351 - +0.9 3,663,368 + PP_3227 0.39 -0.4 3,663,894 + +0.3 3,663,971 + -0.2 3,663,992 - +0.5 3,664,215 + PP_3228 0.11 -0.8 3,664,264 + +0.9 3,664,423 + PP_3228 0.32 -2.0 3,664,789 + PP_3228 0.70 -1.3 3,664,790 - PP_3228 0.70 +0.0 3,664,839 + PP_3228 0.75 +0.2 3,664,844 - PP_3228 0.76 -1.2
Or see this region's nucleotide sequence