Strain Fitness in Pseudomonas putida KT2440 around PP_3221

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
3,655,503	+		PP_3220	0.45	-0.6
3,655,503	+		PP_3220	0.45	-0.6
3,655,503	+				+1.3
3,655,503	+		PP_3220	0.45	+1.4
3,655,504	-		PP_3220	0.45	+0.7
3,655,504	-		PP_3220	0.45	+0.5
3,655,638	+		PP_3220	0.53	+1.7
3,655,810	+		PP_3220	0.64	-1.2
3,655,811	-		PP_3220	0.64	-0.2
3,655,811	-		PP_3220	0.64	-0.4
3,656,161	+		PP_3220	0.86	-1.8
3,656,181	-		PP_3220	0.87	-0.1
3,656,358	-				+1.0
3,656,645	-		PP_3221	0.28	+0.9
3,656,857	+		PP_3221	0.51	-1.8
3,656,990	+		PP_3221	0.66	-0.0
3,656,990	+		PP_3221	0.66	-1.7
3,657,005	+		PP_3221	0.68	-0.5
3,657,142	+		PP_3221	0.83	+1.4
3,657,142	+		PP_3221	0.83	-0.8
3,657,200	+		PP_3221	0.89	-1.5
3,657,283	+				+0.6
3,657,284	-				-0.5
3,657,284	-				-1.8
3,657,345	+				-0.4
3,657,345	+				+0.1
3,657,419	+		PP_3222	0.13	-2.3
3,657,491	+		PP_3222	0.20	+0.7
3,657,492	-		PP_3222	0.20	-0.7
3,657,509	-		PP_3222	0.22	+1.3
3,657,509	-		PP_3222	0.22	-0.7
3,657,621	+		PP_3222	0.34	-2.6
3,657,621	+		PP_3222	0.34	-0.1
3,657,621	+		PP_3222	0.34	-0.2
3,657,622	-		PP_3222	0.34	-0.9
3,657,622	-		PP_3222	0.34	-0.9
3,657,622	-		PP_3222	0.34	-0.8
3,657,622	-				+0.2
3,657,622	-		PP_3222	0.34	+0.3
3,657,622	-		PP_3222	0.34	+0.8
3,657,670	-		PP_3222	0.39	-1.8
3,657,670	-		PP_3222	0.39	+1.9
3,658,038	+		PP_3222	0.78	-2.8
3,658,039	-		PP_3222	0.78	+0.7
3,658,039	-				+1.0
3,658,069	-				+0.9
3,658,069	-		PP_3222	0.81	+0.3
3,658,071	+		PP_3222	0.81	-0.8
3,658,072	-		PP_3222	0.81	-2.6
3,658,072	-		PP_3222	0.81	-0.8
3,658,179	+				-0.7
3,658,180	-				+0.4
3,658,180	-				+0.1
3,658,180	-				-2.2
3,658,180	-				+1.1
3,658,189	+				+0.2
3,658,189	+				-1.2
3,658,189	+				-1.6
3,658,189	+				-0.2
3,658,189	+				+0.7
3,658,190	-				+1.2
3,658,190	-				-0.8
3,658,190	-				+0.2
3,658,190	-				-2.7
3,658,190	-				+0.3
3,658,191	+				+0.8
3,658,191	+				-0.3
3,658,191	+				-0.4
3,658,191	+				+1.4
3,658,192	-				-0.2
3,658,192	-				-0.4
3,658,192	-				-0.9