Strain Fitness in Pseudomonas putida KT2440 around PP_3220

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
3,653,876	+		PP_3219	0.31	-0.1
3,653,877	-		PP_3219	0.31	+0.8
3,653,877	-		PP_3219	0.31	-2.0
3,654,061	+		PP_3219	0.48	-0.4
3,654,062	-		PP_3219	0.48	-0.1
3,654,062	-		PP_3219	0.48	+1.0
3,654,090	+		PP_3219	0.51	+3.2
3,654,118	+		PP_3219	0.53	+0.3
3,654,218	+		PP_3219	0.62	-1.6
3,654,235	+		PP_3219	0.64	-0.5
3,654,246	-		PP_3219	0.65	-1.1
3,654,246	-		PP_3219	0.65	-0.7
3,654,246	-		PP_3219	0.65	+0.2
3,654,449	-		PP_3219	0.83	+0.2
3,654,482	+		PP_3219	0.86	+1.1
3,654,517	+		PP_3219	0.89	+1.1
3,654,695	-				-0.3
3,654,707	+				+0.0
3,654,708	-				+1.1
3,654,708	-				+0.8
3,654,774	+				-0.3
3,654,838	-				-0.2
3,654,902	+				+0.4
3,654,902	+				+0.2
3,654,963	+		PP_3220	0.12	-0.0
3,654,963	+		PP_3220	0.12	+0.0
3,654,964	-		PP_3220	0.12	+1.3
3,654,964	-		PP_3220	0.12	-0.1
3,654,964	-		PP_3220	0.12	-0.8
3,655,197	-		PP_3220	0.26	+0.9
3,655,341	+		PP_3220	0.35	-2.1
3,655,503	+		PP_3220	0.45	-0.6
3,655,503	+		PP_3220	0.45	-0.6
3,655,503	+				+1.3
3,655,503	+		PP_3220	0.45	+1.4
3,655,504	-		PP_3220	0.45	+0.7
3,655,504	-		PP_3220	0.45	+0.5
3,655,638	+		PP_3220	0.53	+1.7
3,655,810	+		PP_3220	0.64	-1.2
3,655,811	-		PP_3220	0.64	-0.2
3,655,811	-		PP_3220	0.64	-0.4
3,656,161	+		PP_3220	0.86	-1.8
3,656,181	-		PP_3220	0.87	-0.1
3,656,358	-				+1.0
3,656,645	-		PP_3221	0.28	+0.9
3,656,857	+		PP_3221	0.51	-1.8
3,656,990	+		PP_3221	0.66	-0.0
3,656,990	+		PP_3221	0.66	-1.7
3,657,005	+		PP_3221	0.68	-0.5
3,657,142	+		PP_3221	0.83	+1.4
3,657,142	+		PP_3221	0.83	-0.8
3,657,200	+		PP_3221	0.89	-1.5
3,657,283	+				+0.6
3,657,284	-				-0.5
3,657,284	-				-1.8
3,657,345	+				-0.4
3,657,345	+				+0.1