Strain Fitness in Pseudomonas putida KT2440 around PP_3218

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
3,651,126	-				+0.9
3,651,250	-		PP_3217	0.14	+0.3
3,651,250	-		PP_3217	0.14	-1.5
3,651,264	-		PP_3217	0.15	-0.2
3,651,264	-		PP_3217	0.15	-0.8
3,651,581	+		PP_3217	0.49	-1.2
3,651,582	-		PP_3217	0.49	+1.7
3,651,653	+		PP_3217	0.57	-1.0
3,651,653	+		PP_3217	0.57	-0.3
3,651,653	+		PP_3217	0.57	-1.3
3,651,653	+		PP_3217	0.57	+1.0
3,651,653	+		PP_3217	0.57	+1.2
3,651,654	-		PP_3217	0.57	+1.2
3,651,654	-		PP_3217	0.57	+1.9
3,651,654	-		PP_3217	0.57	+0.5
3,651,654	-		PP_3217	0.57	-1.0
3,651,654	-		PP_3217	0.57	+0.5
3,651,654	-		PP_3217	0.57	-0.0
3,651,842	+		PP_3217	0.77	+0.2
3,651,843	-		PP_3217	0.77	-1.5
3,652,051	+				+0.2
3,652,052	-				+0.8
3,652,055	+				+0.4
3,652,055	+				-0.3
3,652,056	-				+0.8
3,652,090	+				+2.1
3,652,091	-				-0.2
3,652,091	-				+2.9
3,652,091	-				-0.7
3,652,232	+				+0.3
3,652,232	+				+0.6
3,652,415	+		PP_3218	0.23	+0.8
3,652,416	-		PP_3218	0.23	+1.1
3,652,416	-		PP_3218	0.23	-0.7
3,652,527	-		PP_3218	0.31	-1.6
3,652,527	-		PP_3218	0.31	+0.5
3,652,544	+		PP_3218	0.33	-0.7
3,652,544	+		PP_3218	0.33	-0.1
3,652,544	+				+1.7
3,652,550	+				+2.4
3,652,551	-		PP_3218	0.33	-0.8
3,652,562	+		PP_3218	0.34	-0.9
3,652,859	+		PP_3218	0.56	-0.8
3,652,918	-		PP_3218	0.61	-2.7
3,652,937	+		PP_3218	0.62	+0.3
3,652,937	+		PP_3218	0.62	-0.5
3,652,938	-		PP_3218	0.62	-0.1
3,652,982	+		PP_3218	0.66	-0.2
3,653,060	+		PP_3218	0.72	+0.2
3,653,060	+		PP_3218	0.72	-0.5
3,653,199	+		PP_3218	0.82	-0.8
3,653,288	-		PP_3218	0.89	-1.1
3,653,288	-		PP_3218	0.89	+0.1
3,653,291	+		PP_3218	0.89	-2.3
3,653,291	+		PP_3218	0.89	-0.4
3,653,291	+		PP_3218	0.89	-1.0
3,653,291	+		PP_3218	0.89	+1.8
3,653,291	+		PP_3218	0.89	+0.9
3,653,291	+		PP_3218	0.89	-1.3
3,653,292	-		PP_3218	0.89	-0.1
3,653,292	-		PP_3218	0.89	-1.6
3,653,292	-		PP_3218	0.89	-0.5
3,653,337	+				+0.8
3,653,364	-				-1.4
3,653,364	-				-0.6
3,653,375	+				+0.5
3,653,375	+				-0.3
3,653,432	+				+0.4
3,653,433	-				+0.6
3,653,571	+				+0.2
3,653,608	+				-0.6
3,653,671	+		PP_3219	0.13	+2.0
3,653,672	-		PP_3219	0.13	-1.4
3,653,674	+		PP_3219	0.13	+0.3
3,653,675	-		PP_3219	0.13	-1.4
3,653,729	+		PP_3219	0.18	-0.8
3,653,730	-		PP_3219	0.18	-1.2
3,653,876	+		PP_3219	0.31	-0.1
3,653,877	-		PP_3219	0.31	+0.8
3,653,877	-		PP_3219	0.31	-2.0
3,654,061	+		PP_3219	0.48	-0.4
3,654,062	-		PP_3219	0.48	-0.1
3,654,062	-		PP_3219	0.48	+1.0
3,654,090	+		PP_3219	0.51	+3.2
3,654,118	+		PP_3219	0.53	+0.3
3,654,218	+		PP_3219	0.62	-1.6
3,654,235	+		PP_3219	0.64	-0.5
3,654,246	-		PP_3219	0.65	-1.1
3,654,246	-		PP_3219	0.65	-0.7
3,654,246	-		PP_3219	0.65	+0.2