Strain Fitness in Pseudomonas putida KT2440 around PP_3182

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
3,605,884	+				-1.5
3,605,884	+				+0.1
3,605,884	+				-1.3
3,605,885	-				-0.6
3,605,920	+		PP_3181	0.11	+0.0
3,605,920	+		PP_3181	0.11	+0.3
3,605,920	+		PP_3181	0.11	-0.1
3,605,921	-		PP_3181	0.12	-0.3
3,605,996	+		PP_3181	0.19	-2.3
3,605,998	+		PP_3181	0.20	+1.0
3,605,998	+		PP_3181	0.20	-1.1
3,605,998	+		PP_3181	0.20	+0.2
3,605,998	+		PP_3181	0.20	-0.0
3,605,999	-		PP_3181	0.20	-0.5
3,606,502	-		PP_3181	0.73	-1.0
3,606,593	+		PP_3181	0.82	-0.1
3,606,629	-		PP_3181	0.86	-1.1
3,606,639	-		PP_3181	0.87	+0.4
3,606,703	+				-1.2
3,606,703	+				-1.5
3,606,704	-				-0.8
3,606,704	-				+0.5
3,606,742	+				-0.1
3,606,743	-				-1.8
3,606,743	-				-2.3
3,606,795	-				+0.2
3,606,856	+				+0.4
3,606,856	+				+0.2
3,606,857	-				+0.5
3,606,938	-				+0.7
3,606,973	+				+0.8
3,607,208	+		PP_3182	0.16	+1.3
3,607,208	+		PP_3182	0.16	+0.0
3,607,209	-		PP_3182	0.16	+0.9
3,607,209	-		PP_3182	0.16	+0.2
3,607,209	-		PP_3182	0.16	-1.3
3,607,429	-		PP_3182	0.25	-0.7
3,607,449	+		PP_3182	0.26	+0.4
3,607,451	+		PP_3182	0.26	-1.5
3,607,451	+		PP_3182	0.26	-0.8
3,607,580	-		PP_3182	0.32	-2.0
3,607,711	+		PP_3182	0.38	+0.5
3,607,712	-		PP_3182	0.38	-1.3
3,607,712	-		PP_3182	0.38	-0.0
3,607,783	-		PP_3182	0.41	-0.5
3,607,822	+		PP_3182	0.43	-1.6
3,607,823	-		PP_3182	0.43	-2.1
3,607,980	+		PP_3182	0.50	-0.8
3,607,981	-		PP_3182	0.50	+0.3
3,608,277	+		PP_3182	0.63	-0.3
3,608,278	-		PP_3182	0.63	-0.5
3,608,335	+		PP_3182	0.65	-1.7
3,608,335	+		PP_3182	0.65	-0.0
3,608,446	+		PP_3182	0.70	+0.4
3,608,447	-		PP_3182	0.70	-0.0
3,608,738	+		PP_3182	0.83	-0.3
3,608,739	-		PP_3182	0.83	-1.3
3,608,739	-		PP_3182	0.83	-3.0
3,608,832	-		PP_3182	0.87	-0.6
3,608,867	-		PP_3182	0.89	-2.2
3,609,175	-				+1.2
3,609,175	-				+0.0
3,609,175	-				+1.0
3,609,213	+				-1.5
3,609,214	-				+1.3
3,609,214	-				-0.1
3,609,244	-				-2.2
3,609,250	+				-1.4
3,609,251	-				-0.0
3,609,264	-				-0.6
3,609,308	-				-0.4
3,609,308	-				-1.8
3,609,312	+				-0.6
3,609,370	+		PP_3183	0.12	-0.6
3,609,371	-		PP_3183	0.12	+1.2
3,609,371	-		PP_3183	0.12	-0.6
3,609,371	-		PP_3183	0.12	+0.4
3,609,371	-		PP_3183	0.12	-0.1
3,609,670	+		PP_3183	0.43	+0.2
3,609,671	-		PP_3183	0.43	-0.5
3,609,798	+		PP_3183	0.56	+1.4
3,609,799	-		PP_3183	0.56	+0.5
3,609,799	-		PP_3183	0.56	-0.5
3,609,868	+		PP_3183	0.63	+0.7
3,609,869	-		PP_3183	0.63	+1.1
3,609,869	-		PP_3183	0.63	+0.7
3,609,919	+		PP_3183	0.68	-0.5
3,609,963	+		PP_3183	0.73	-1.0
3,609,964	-		PP_3183	0.73	+0.4
3,609,964	-		PP_3183	0.73	-1.6
3,610,112	-		PP_3183	0.88	+0.7
3,610,112	-		PP_3183	0.88	+0.5