Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5589 and PP_3159 are separated by 634 nucleotides PP_3159 and PP_3160 are separated by 82 nucleotides
PP_5589: PP_5589 - protein of unknown function containing Rhs repeat-associated core domain, at 3,578,647 to 3,579,231
_5589
PP_3159: PP_3159 - BenABC operon transcriptional activator, at 3,579,866 to 3,580,822
_3159
PP_3160: PP_3160 - conserved protein of unknown function, at 3,580,905 to 3,581,846
_3160
Position (kb)
3579
3580
3581 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3579.040 kb on - strand, within PP_5589 at 3579.113 kb on + strand, within PP_5589 at 3579.141 kb on + strand, within PP_5589 at 3579.148 kb on - strand at 3579.329 kb on - strand at 3579.346 kb on - strand at 3579.352 kb on + strand at 3579.359 kb on + strand at 3579.383 kb on - strand at 3579.426 kb on - strand at 3579.426 kb on - strand at 3579.426 kb on - strand at 3579.512 kb on + strand at 3579.513 kb on - strand at 3579.548 kb on + strand at 3579.600 kb on + strand at 3579.601 kb on - strand at 3579.619 kb on + strand at 3579.629 kb on + strand at 3579.630 kb on - strand at 3579.725 kb on + strand at 3579.725 kb on + strand at 3579.725 kb on + strand at 3579.726 kb on - strand at 3579.726 kb on - strand at 3579.780 kb on + strand at 3579.821 kb on + strand at 3579.822 kb on - strand at 3579.896 kb on - strand at 3579.896 kb on - strand at 3579.896 kb on - strand at 3579.896 kb on - strand at 3579.920 kb on + strand at 3579.920 kb on + strand at 3579.921 kb on - strand at 3579.921 kb on - strand at 3579.935 kb on + strand at 3579.935 kb on + strand at 3579.936 kb on - strand at 3579.952 kb on - strand at 3579.952 kb on - strand at 3579.952 kb on - strand at 3579.952 kb on - strand at 3579.952 kb on - strand at 3580.047 kb on - strand, within PP_3159 at 3580.047 kb on - strand, within PP_3159 at 3580.091 kb on + strand, within PP_3159 at 3580.092 kb on - strand, within PP_3159 at 3580.092 kb on - strand, within PP_3159 at 3580.138 kb on + strand, within PP_3159 at 3580.138 kb on + strand, within PP_3159 at 3580.139 kb on - strand, within PP_3159 at 3580.209 kb on - strand, within PP_3159 at 3580.421 kb on + strand, within PP_3159 at 3580.422 kb on - strand, within PP_3159 at 3580.506 kb on + strand, within PP_3159 at 3580.524 kb on - strand, within PP_3159 at 3580.524 kb on - strand, within PP_3159 at 3580.601 kb on + strand, within PP_3159 at 3580.602 kb on - strand, within PP_3159 at 3580.693 kb on + strand, within PP_3159 at 3580.694 kb on - strand, within PP_3159 at 3580.838 kb on - strand at 3580.838 kb on - strand at 3580.850 kb on + strand at 3580.850 kb on + strand at 3580.850 kb on + strand at 3580.851 kb on - strand at 3580.887 kb on + strand at 3580.887 kb on + strand at 3580.888 kb on - strand at 3580.893 kb on + strand at 3580.894 kb on - strand at 3580.947 kb on + strand at 3580.948 kb on - strand at 3580.977 kb on + strand at 3580.977 kb on + strand at 3580.978 kb on - strand at 3581.058 kb on + strand, within PP_3160 at 3581.071 kb on + strand, within PP_3160 at 3581.214 kb on + strand, within PP_3160 at 3581.214 kb on + strand, within PP_3160 at 3581.215 kb on - strand, within PP_3160 at 3581.314 kb on - strand, within PP_3160 at 3581.314 kb on - strand, within PP_3160 at 3581.314 kb on - strand, within PP_3160 at 3581.319 kb on + strand, within PP_3160 at 3581.385 kb on + strand, within PP_3160 at 3581.421 kb on - strand, within PP_3160 at 3581.421 kb on - strand, within PP_3160 at 3581.454 kb on + strand, within PP_3160 at 3581.455 kb on - strand, within PP_3160 at 3581.525 kb on + strand, within PP_3160 at 3581.584 kb on + strand, within PP_3160 at 3581.585 kb on - strand, within PP_3160 at 3581.716 kb on + strand, within PP_3160 at 3581.717 kb on - strand, within PP_3160 at 3581.717 kb on - strand, within PP_3160 at 3581.717 kb on - strand, within PP_3160 at 3581.717 kb on - strand, within PP_3160 at 3581.717 kb on - strand, within PP_3160 at 3581.717 kb on - strand, within PP_3160 at 3581.717 kb on - strand, within PP_3160 at 3581.767 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,579,040 - PP_5589 0.67 +0.2 3,579,113 + PP_5589 0.80 +0.3 3,579,141 + PP_5589 0.84 +0.7 3,579,148 - +1.0 3,579,329 - -1.5 3,579,346 - -0.8 3,579,352 + +1.2 3,579,359 + -0.6 3,579,383 - -0.2 3,579,426 - -1.2 3,579,426 - -0.4 3,579,426 - -1.9 3,579,512 + +0.9 3,579,513 - +0.2 3,579,548 + -0.9 3,579,600 + -2.0 3,579,601 - +0.3 3,579,619 + -0.3 3,579,629 + +1.5 3,579,630 - +1.3 3,579,725 + -0.8 3,579,725 + +1.5 3,579,725 + -1.2 3,579,726 - +0.2 3,579,726 - -1.6 3,579,780 + +0.1 3,579,821 + +0.3 3,579,822 - +1.9 3,579,896 - -0.5 3,579,896 - +2.0 3,579,896 - +1.1 3,579,896 - -0.7 3,579,920 + -0.8 3,579,920 + -0.3 3,579,921 - +1.2 3,579,921 - -1.2 3,579,935 + +0.7 3,579,935 + +1.1 3,579,936 - -0.3 3,579,952 - +1.4 3,579,952 - -2.1 3,579,952 - +0.5 3,579,952 - -0.5 3,579,952 - -0.1 3,580,047 - PP_3159 0.19 -1.5 3,580,047 - PP_3159 0.19 -0.3 3,580,091 + PP_3159 0.24 -0.8 3,580,092 - PP_3159 0.24 +1.0 3,580,092 - PP_3159 0.24 -1.1 3,580,138 + PP_3159 0.28 -0.9 3,580,138 + PP_3159 0.28 +0.1 3,580,139 - PP_3159 0.29 -2.1 3,580,209 - PP_3159 0.36 +0.6 3,580,421 + PP_3159 0.58 -0.1 3,580,422 - PP_3159 0.58 -1.1 3,580,506 + PP_3159 0.67 +0.1 3,580,524 - PP_3159 0.69 -1.6 3,580,524 - PP_3159 0.69 +0.5 3,580,601 + PP_3159 0.77 -1.8 3,580,602 - PP_3159 0.77 -1.7 3,580,693 + PP_3159 0.86 -0.1 3,580,694 - PP_3159 0.87 +0.2 3,580,838 - -0.3 3,580,838 - +1.0 3,580,850 + -0.2 3,580,850 + -0.4 3,580,850 + +0.6 3,580,851 - +1.5 3,580,887 + +1.5 3,580,887 + -1.8 3,580,888 - -1.0 3,580,893 + -1.4 3,580,894 - -0.8 3,580,947 + -0.0 3,580,948 - -0.2 3,580,977 + -1.5 3,580,977 + +0.8 3,580,978 - +0.9 3,581,058 + PP_3160 0.16 +0.5 3,581,071 + PP_3160 0.18 +0.9 3,581,214 + PP_3160 0.33 +2.4 3,581,214 + PP_3160 0.33 -1.1 3,581,215 - PP_3160 0.33 -2.1 3,581,314 - PP_3160 0.43 -0.8 3,581,314 - PP_3160 0.43 -0.5 3,581,314 - PP_3160 0.43 +0.0 3,581,319 + PP_3160 0.44 +0.6 3,581,385 + PP_3160 0.51 +1.0 3,581,421 - PP_3160 0.55 +1.4 3,581,421 - PP_3160 0.55 -0.2 3,581,454 + PP_3160 0.58 -0.5 3,581,455 - PP_3160 0.58 +1.0 3,581,525 + PP_3160 0.66 -0.5 3,581,584 + PP_3160 0.72 -1.2 3,581,585 - PP_3160 0.72 -0.4 3,581,716 + PP_3160 0.86 +0.6 3,581,717 - PP_3160 0.86 +0.1 3,581,717 - PP_3160 0.86 -2.0 3,581,717 - PP_3160 0.86 -0.5 3,581,717 - PP_3160 0.86 +1.9 3,581,717 - PP_3160 0.86 +0.5 3,581,717 - PP_3160 0.86 +0.0 3,581,717 - PP_3160 0.86 +1.7 3,581,767 + -2.0
Or see this region's nucleotide sequence