Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3144 and PP_3145 are separated by 87 nucleotides PP_3145 and PP_3146 overlap by 4 nucleotides
PP_3144: PP_3144 - L-serine dehydratase, at 3,560,246 to 3,561,622
_3144
PP_3145: PP_3145 - conserved protein of unknown function, at 3,561,710 to 3,562,531
_3145
PP_3146: PP_3146 - putative Oxidoreductase, at 3,562,528 to 3,563,823
_3146
Position (kb)
3561
3562
3563 Strain fitness (log2 ratio)
-2
-1
0
1 at 3560.757 kb on - strand, within PP_3144 at 3561.097 kb on + strand, within PP_3144 at 3561.097 kb on + strand, within PP_3144 at 3561.097 kb on + strand, within PP_3144 at 3561.152 kb on + strand, within PP_3144 at 3561.152 kb on + strand, within PP_3144 at 3561.155 kb on + strand, within PP_3144 at 3561.155 kb on + strand, within PP_3144 at 3561.155 kb on + strand, within PP_3144 at 3561.155 kb on + strand, within PP_3144 at 3561.155 kb on + strand, within PP_3144 at 3561.156 kb on - strand, within PP_3144 at 3561.156 kb on - strand, within PP_3144 at 3561.548 kb on - strand at 3561.572 kb on + strand at 3561.573 kb on - strand at 3561.573 kb on - strand at 3561.627 kb on + strand at 3561.857 kb on + strand, within PP_3145 at 3561.858 kb on - strand, within PP_3145 at 3561.975 kb on + strand, within PP_3145 at 3561.994 kb on + strand, within PP_3145 at 3562.138 kb on + strand, within PP_3145 at 3562.197 kb on + strand, within PP_3145 at 3562.197 kb on + strand at 3562.197 kb on + strand, within PP_3145 at 3562.207 kb on + strand, within PP_3145 at 3562.279 kb on - strand, within PP_3145 at 3562.427 kb on + strand, within PP_3145 at 3562.461 kb on + strand at 3562.734 kb on - strand, within PP_3146 at 3562.736 kb on + strand at 3562.744 kb on - strand, within PP_3146 at 3562.814 kb on + strand, within PP_3146 at 3562.847 kb on - strand, within PP_3146 at 3562.871 kb on + strand, within PP_3146 at 3562.872 kb on - strand, within PP_3146 at 3562.955 kb on + strand, within PP_3146 at 3562.955 kb on + strand, within PP_3146 at 3562.956 kb on - strand, within PP_3146 at 3562.956 kb on - strand, within PP_3146 at 3563.048 kb on + strand, within PP_3146 at 3563.048 kb on + strand, within PP_3146 at 3563.048 kb on + strand, within PP_3146 at 3563.049 kb on - strand, within PP_3146 at 3563.049 kb on - strand, within PP_3146 at 3563.049 kb on - strand, within PP_3146 at 3563.066 kb on - strand, within PP_3146 at 3563.531 kb on + strand, within PP_3146
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,560,757 - PP_3144 0.37 -0.9 3,561,097 + PP_3144 0.62 +0.1 3,561,097 + PP_3144 0.62 +0.4 3,561,097 + PP_3144 0.62 +0.4 3,561,152 + PP_3144 0.66 +0.8 3,561,152 + PP_3144 0.66 +0.5 3,561,155 + PP_3144 0.66 -1.0 3,561,155 + PP_3144 0.66 +0.6 3,561,155 + PP_3144 0.66 -0.5 3,561,155 + PP_3144 0.66 +0.4 3,561,155 + PP_3144 0.66 -0.7 3,561,156 - PP_3144 0.66 -1.6 3,561,156 - PP_3144 0.66 +1.1 3,561,548 - +0.5 3,561,572 + +0.3 3,561,573 - +0.3 3,561,573 - +1.5 3,561,627 + +0.7 3,561,857 + PP_3145 0.18 +0.7 3,561,858 - PP_3145 0.18 +1.2 3,561,975 + PP_3145 0.32 +1.3 3,561,994 + PP_3145 0.35 -0.5 3,562,138 + PP_3145 0.52 -0.1 3,562,197 + PP_3145 0.59 -1.3 3,562,197 + +1.2 3,562,197 + PP_3145 0.59 +0.1 3,562,207 + PP_3145 0.60 +0.9 3,562,279 - PP_3145 0.69 +0.7 3,562,427 + PP_3145 0.87 +1.0 3,562,461 + +0.3 3,562,734 - PP_3146 0.16 -1.6 3,562,736 + +1.5 3,562,744 - PP_3146 0.17 -0.3 3,562,814 + PP_3146 0.22 -2.1 3,562,847 - PP_3146 0.25 -0.3 3,562,871 + PP_3146 0.26 -0.9 3,562,872 - PP_3146 0.27 -1.3 3,562,955 + PP_3146 0.33 +0.6 3,562,955 + PP_3146 0.33 +1.1 3,562,956 - PP_3146 0.33 -0.0 3,562,956 - PP_3146 0.33 +0.4 3,563,048 + PP_3146 0.40 -0.3 3,563,048 + PP_3146 0.40 -1.2 3,563,048 + PP_3146 0.40 -1.1 3,563,049 - PP_3146 0.40 +0.4 3,563,049 - PP_3146 0.40 -1.6 3,563,049 - PP_3146 0.40 +0.8 3,563,066 - PP_3146 0.42 +1.5 3,563,531 + PP_3146 0.77 -0.4
Or see this region's nucleotide sequence