Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3081 and PP_5560 are separated by 123 nucleotides PP_5560 and PP_3082 are separated by 81 nucleotides PP_3082 and PP_3083 are separated by 360 nucleotides
PP_3081: PP_3081 - conserved protein of unknown function, at 3,466,349 to 3,466,825
_3081
PP_5560: PP_5560 - conserved exported protein of unknown function, at 3,466,949 to 3,467,257
_5560
PP_3082: PP_3082 - putative Membrane protein, at 3,467,339 to 3,468,703
_3082
PP_3083: PP_3083 - putative Membrane protein, at 3,469,064 to 3,469,939
_3083
Position (kb)
3467
3468
3469 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3466.483 kb on - strand, within PP_3081 at 3466.525 kb on - strand, within PP_3081 at 3466.623 kb on + strand, within PP_3081 at 3466.623 kb on + strand, within PP_3081 at 3466.624 kb on - strand, within PP_3081 at 3467.795 kb on + strand, within PP_3082 at 3467.961 kb on + strand, within PP_3082 at 3467.961 kb on + strand, within PP_3082 at 3467.962 kb on - strand, within PP_3082 at 3467.979 kb on + strand, within PP_3082 at 3467.979 kb on + strand, within PP_3082 at 3467.980 kb on - strand, within PP_3082 at 3467.980 kb on - strand, within PP_3082 at 3468.216 kb on + strand, within PP_3082 at 3468.231 kb on + strand, within PP_3082 at 3468.231 kb on + strand at 3468.232 kb on - strand, within PP_3082 at 3468.335 kb on + strand, within PP_3082 at 3468.336 kb on - strand, within PP_3082 at 3468.361 kb on - strand, within PP_3082 at 3468.361 kb on - strand, within PP_3082 at 3468.414 kb on + strand, within PP_3082 at 3468.486 kb on + strand, within PP_3082 at 3468.486 kb on + strand, within PP_3082 at 3468.486 kb on + strand, within PP_3082 at 3468.486 kb on + strand, within PP_3082 at 3468.487 kb on - strand, within PP_3082 at 3468.487 kb on - strand, within PP_3082 at 3468.637 kb on + strand at 3468.637 kb on + strand at 3468.637 kb on + strand at 3468.637 kb on + strand at 3468.637 kb on + strand at 3468.637 kb on + strand at 3468.637 kb on + strand at 3468.637 kb on + strand at 3468.637 kb on + strand at 3468.637 kb on + strand at 3468.637 kb on + strand at 3468.637 kb on + strand at 3468.638 kb on - strand at 3468.638 kb on - strand at 3468.638 kb on - strand at 3468.638 kb on - strand at 3468.638 kb on - strand at 3468.638 kb on - strand at 3468.638 kb on - strand at 3468.638 kb on - strand at 3468.641 kb on + strand at 3468.642 kb on - strand at 3468.642 kb on - strand at 3468.642 kb on - strand at 3468.642 kb on - strand at 3468.642 kb on - strand at 3468.791 kb on - strand at 3469.034 kb on + strand at 3469.034 kb on + strand at 3469.034 kb on + strand at 3469.034 kb on + strand at 3469.035 kb on - strand at 3469.035 kb on - strand at 3469.176 kb on + strand, within PP_3083 at 3469.176 kb on + strand, within PP_3083 at 3469.397 kb on + strand, within PP_3083 at 3469.397 kb on + strand, within PP_3083 at 3469.398 kb on - strand, within PP_3083 at 3469.398 kb on - strand, within PP_3083 at 3469.398 kb on - strand, within PP_3083 at 3469.526 kb on - strand, within PP_3083 at 3469.526 kb on - strand, within PP_3083
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,466,483 - PP_3081 0.28 -0.9 3,466,525 - PP_3081 0.37 -1.8 3,466,623 + PP_3081 0.57 +0.6 3,466,623 + PP_3081 0.57 -0.3 3,466,624 - PP_3081 0.58 -0.6 3,467,795 + PP_3082 0.33 +0.6 3,467,961 + PP_3082 0.46 +1.4 3,467,961 + PP_3082 0.46 -0.2 3,467,962 - PP_3082 0.46 -0.9 3,467,979 + PP_3082 0.47 -1.8 3,467,979 + PP_3082 0.47 -0.6 3,467,980 - PP_3082 0.47 -2.2 3,467,980 - PP_3082 0.47 -0.3 3,468,216 + PP_3082 0.64 -1.8 3,468,231 + PP_3082 0.65 +0.3 3,468,231 + +0.5 3,468,232 - PP_3082 0.65 -2.1 3,468,335 + PP_3082 0.73 +1.0 3,468,336 - PP_3082 0.73 -0.2 3,468,361 - PP_3082 0.75 -1.0 3,468,361 - PP_3082 0.75 -0.9 3,468,414 + PP_3082 0.79 +0.7 3,468,486 + PP_3082 0.84 -1.8 3,468,486 + PP_3082 0.84 -0.5 3,468,486 + PP_3082 0.84 +1.0 3,468,486 + PP_3082 0.84 -0.1 3,468,487 - PP_3082 0.84 -1.8 3,468,487 - PP_3082 0.84 +0.5 3,468,637 + +0.9 3,468,637 + +0.7 3,468,637 + -0.3 3,468,637 + +0.6 3,468,637 + -0.7 3,468,637 + -0.5 3,468,637 + -0.7 3,468,637 + +2.6 3,468,637 + -0.6 3,468,637 + +1.2 3,468,637 + +0.3 3,468,637 + -0.7 3,468,638 - -1.2 3,468,638 - +0.6 3,468,638 - +0.1 3,468,638 - +0.1 3,468,638 - -0.5 3,468,638 - -2.7 3,468,638 - +0.6 3,468,638 - -0.3 3,468,641 + -0.7 3,468,642 - -0.3 3,468,642 - +0.7 3,468,642 - -2.4 3,468,642 - -0.1 3,468,642 - -0.4 3,468,791 - -1.6 3,469,034 + +1.8 3,469,034 + +0.8 3,469,034 + -0.8 3,469,034 + -2.5 3,469,035 - -0.0 3,469,035 - +0.6 3,469,176 + PP_3083 0.13 +0.1 3,469,176 + PP_3083 0.13 -1.9 3,469,397 + PP_3083 0.38 -0.2 3,469,397 + PP_3083 0.38 -0.1 3,469,398 - PP_3083 0.38 +0.4 3,469,398 - PP_3083 0.38 +0.2 3,469,398 - PP_3083 0.38 -0.4 3,469,526 - PP_3083 0.53 -1.0 3,469,526 - PP_3083 0.53 -1.6
Or see this region's nucleotide sequence