Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3080 and PP_5559 are separated by 334 nucleotides PP_5559 and PP_3081 are separated by 100 nucleotides PP_3081 and PP_5560 are separated by 123 nucleotides PP_5560 and PP_3082 are separated by 81 nucleotides
PP_3080: PP_3080 - tyr-sensitive phospho-2-dehydro-3-deoxyheptonate aldolase, at 3,464,733 to 3,465,794
_3080
PP_5559: PP_5559 - conserved protein of unknown function, at 3,466,129 to 3,466,248
_5559
PP_3081: PP_3081 - conserved protein of unknown function, at 3,466,349 to 3,466,825
_3081
PP_5560: PP_5560 - conserved exported protein of unknown function, at 3,466,949 to 3,467,257
_5560
PP_3082: PP_3082 - putative Membrane protein, at 3,467,339 to 3,468,703
_3082
Position (kb)
3466
3467 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3465.470 kb on + strand, within PP_3080 at 3465.470 kb on + strand, within PP_3080 at 3465.470 kb on + strand, within PP_3080 at 3465.471 kb on - strand, within PP_3080 at 3465.471 kb on - strand, within PP_3080 at 3465.471 kb on - strand, within PP_3080 at 3465.474 kb on - strand, within PP_3080 at 3465.491 kb on - strand, within PP_3080 at 3465.556 kb on + strand, within PP_3080 at 3465.556 kb on + strand, within PP_3080 at 3465.844 kb on + strand at 3465.876 kb on - strand at 3465.899 kb on + strand at 3465.900 kb on - strand at 3465.958 kb on + strand at 3465.959 kb on - strand at 3465.971 kb on - strand at 3465.971 kb on - strand at 3465.971 kb on - strand at 3465.974 kb on - strand at 3465.976 kb on + strand at 3465.976 kb on + strand at 3465.976 kb on + strand at 3465.976 kb on + strand at 3465.977 kb on - strand at 3465.977 kb on - strand at 3465.977 kb on - strand at 3465.985 kb on + strand at 3465.986 kb on - strand at 3466.137 kb on + strand at 3466.157 kb on + strand, within PP_5559 at 3466.278 kb on - strand at 3466.307 kb on + strand at 3466.314 kb on + strand at 3466.314 kb on + strand at 3466.315 kb on - strand at 3466.483 kb on - strand, within PP_3081 at 3466.525 kb on - strand, within PP_3081 at 3466.623 kb on + strand, within PP_3081 at 3466.623 kb on + strand, within PP_3081 at 3466.624 kb on - strand, within PP_3081 at 3467.795 kb on + strand, within PP_3082
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,465,470 + PP_3080 0.69 -0.8 3,465,470 + PP_3080 0.69 -0.7 3,465,470 + PP_3080 0.69 -1.4 3,465,471 - PP_3080 0.69 +0.2 3,465,471 - PP_3080 0.69 +0.8 3,465,471 - PP_3080 0.69 -2.1 3,465,474 - PP_3080 0.70 -1.3 3,465,491 - PP_3080 0.71 -2.3 3,465,556 + PP_3080 0.77 -0.7 3,465,556 + PP_3080 0.77 +0.3 3,465,844 + -2.3 3,465,876 - -1.2 3,465,899 + -0.2 3,465,900 - +2.1 3,465,958 + -1.9 3,465,959 - -0.4 3,465,971 - -0.4 3,465,971 - +1.4 3,465,971 - -0.1 3,465,974 - +0.6 3,465,976 + +2.4 3,465,976 + +1.8 3,465,976 + -0.6 3,465,976 + +0.5 3,465,977 - -1.7 3,465,977 - +0.4 3,465,977 - +0.2 3,465,985 + +0.1 3,465,986 - +0.2 3,466,137 + -1.5 3,466,157 + PP_5559 0.23 +1.4 3,466,278 - -1.2 3,466,307 + -2.1 3,466,314 + +0.5 3,466,314 + +0.3 3,466,315 - -2.2 3,466,483 - PP_3081 0.28 -0.9 3,466,525 - PP_3081 0.37 -1.8 3,466,623 + PP_3081 0.57 +0.6 3,466,623 + PP_3081 0.57 -0.3 3,466,624 - PP_3081 0.58 -0.6 3,467,795 + PP_3082 0.33 +0.6
Or see this region's nucleotide sequence