Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3062 and PP_3063 are separated by 9 nucleotides PP_3063 and PP_3064 overlap by 26 nucleotides PP_3064 and PP_3065 are separated by 59 nucleotides
PP_3062: PP_3062 - putative Pyocin R2_PP, tail length determination protein, at 3,441,242 to 3,444,511
_3062
PP_3063: PP_3063 - Pyocin R2_PP, tail formation protein, at 3,444,521 to 3,445,366
_3063
PP_3064: PP_3064 - Phage tail protein, at 3,445,341 to 3,445,547
_3064
PP_3065: PP_3065 - Pyocin R2_PP, tail formation, at 3,445,607 to 3,446,650
_3065
Position (kb)
3444
3445
3446 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3443.699 kb on + strand, within PP_3062 at 3443.699 kb on + strand, within PP_3062 at 3443.700 kb on - strand, within PP_3062 at 3443.700 kb on - strand, within PP_3062 at 3443.700 kb on - strand, within PP_3062 at 3443.700 kb on - strand, within PP_3062 at 3443.921 kb on + strand, within PP_3062 at 3443.921 kb on + strand, within PP_3062 at 3443.921 kb on + strand, within PP_3062 at 3444.099 kb on + strand, within PP_3062 at 3444.100 kb on - strand, within PP_3062 at 3444.304 kb on + strand at 3444.304 kb on + strand at 3444.361 kb on + strand at 3444.388 kb on + strand at 3444.510 kb on - strand at 3444.520 kb on + strand at 3444.520 kb on + strand at 3444.521 kb on - strand at 3444.521 kb on - strand at 3444.521 kb on - strand at 3444.527 kb on + strand at 3444.527 kb on + strand at 3444.528 kb on - strand at 3444.676 kb on - strand, within PP_3063 at 3444.761 kb on + strand, within PP_3063 at 3444.761 kb on + strand, within PP_3063 at 3444.762 kb on - strand, within PP_3063 at 3444.762 kb on - strand, within PP_3063 at 3444.762 kb on - strand, within PP_3063 at 3444.996 kb on - strand, within PP_3063 at 3444.996 kb on - strand, within PP_3063 at 3444.998 kb on + strand, within PP_3063 at 3444.999 kb on - strand, within PP_3063 at 3445.154 kb on - strand, within PP_3063 at 3445.322 kb on - strand at 3445.341 kb on - strand at 3445.341 kb on - strand at 3445.341 kb on - strand at 3445.401 kb on + strand, within PP_3064 at 3445.402 kb on - strand, within PP_3064 at 3445.402 kb on - strand, within PP_3064 at 3445.620 kb on - strand at 3445.771 kb on + strand, within PP_3065 at 3445.829 kb on + strand, within PP_3065 at 3445.829 kb on + strand, within PP_3065 at 3445.829 kb on + strand, within PP_3065 at 3445.829 kb on + strand, within PP_3065 at 3445.830 kb on - strand, within PP_3065 at 3445.830 kb on - strand, within PP_3065 at 3445.925 kb on - strand, within PP_3065 at 3446.052 kb on - strand, within PP_3065 at 3446.182 kb on + strand, within PP_3065 at 3446.182 kb on + strand, within PP_3065 at 3446.183 kb on - strand, within PP_3065 at 3446.189 kb on + strand, within PP_3065 at 3446.189 kb on + strand, within PP_3065 at 3446.189 kb on + strand, within PP_3065 at 3446.190 kb on - strand, within PP_3065 at 3446.190 kb on - strand, within PP_3065 at 3446.190 kb on - strand, within PP_3065
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,443,699 + PP_3062 0.75 +0.4 3,443,699 + PP_3062 0.75 -1.2 3,443,700 - PP_3062 0.75 -1.2 3,443,700 - PP_3062 0.75 -0.4 3,443,700 - PP_3062 0.75 +0.3 3,443,700 - PP_3062 0.75 -0.4 3,443,921 + PP_3062 0.82 +0.3 3,443,921 + PP_3062 0.82 +1.3 3,443,921 + PP_3062 0.82 -0.7 3,444,099 + PP_3062 0.87 -0.2 3,444,100 - PP_3062 0.87 -2.2 3,444,304 + -0.2 3,444,304 + +1.3 3,444,361 + -1.4 3,444,388 + -0.8 3,444,510 - +0.6 3,444,520 + -1.2 3,444,520 + -1.1 3,444,521 - +0.4 3,444,521 - +0.3 3,444,521 - -0.2 3,444,527 + -1.8 3,444,527 + -0.4 3,444,528 - +0.6 3,444,676 - PP_3063 0.18 -0.9 3,444,761 + PP_3063 0.28 +1.2 3,444,761 + PP_3063 0.28 +1.2 3,444,762 - PP_3063 0.28 +1.9 3,444,762 - PP_3063 0.28 +0.1 3,444,762 - PP_3063 0.28 +0.2 3,444,996 - PP_3063 0.56 +0.4 3,444,996 - PP_3063 0.56 -0.2 3,444,998 + PP_3063 0.56 -1.6 3,444,999 - PP_3063 0.57 -1.0 3,445,154 - PP_3063 0.75 -0.6 3,445,322 - +1.2 3,445,341 - -0.7 3,445,341 - +0.9 3,445,341 - -0.7 3,445,401 + PP_3064 0.29 -0.3 3,445,402 - PP_3064 0.29 -1.7 3,445,402 - PP_3064 0.29 +0.1 3,445,620 - +0.1 3,445,771 + PP_3065 0.16 +0.1 3,445,829 + PP_3065 0.21 -0.2 3,445,829 + PP_3065 0.21 -2.3 3,445,829 + PP_3065 0.21 -2.6 3,445,829 + PP_3065 0.21 -0.6 3,445,830 - PP_3065 0.21 +1.1 3,445,830 - PP_3065 0.21 +0.2 3,445,925 - PP_3065 0.30 -1.3 3,446,052 - PP_3065 0.43 +1.0 3,446,182 + PP_3065 0.55 +0.3 3,446,182 + PP_3065 0.55 -1.2 3,446,183 - PP_3065 0.55 -0.4 3,446,189 + PP_3065 0.56 -0.5 3,446,189 + PP_3065 0.56 -1.8 3,446,189 + PP_3065 0.56 -1.0 3,446,190 - PP_3065 0.56 +0.6 3,446,190 - PP_3065 0.56 -1.8 3,446,190 - PP_3065 0.56 -1.6
Or see this region's nucleotide sequence