Strain Fitness in Pseudomonas putida KT2440 around PP_3062

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
3,440,243	+				+1.0
3,440,244	-				-1.0
3,440,372	+		PP_3060	0.22	-0.3
3,440,372	+		PP_3060	0.22	+0.7
3,440,373	-		PP_3060	0.23	+0.2
3,440,392	+		PP_3060	0.26	-3.1
3,440,652	-		PP_3060	0.77	+1.1
3,440,652	-		PP_3060	0.77	-1.2
3,440,654	+		PP_3060	0.78	-1.0
3,440,655	-		PP_3060	0.78	-0.7
3,440,685	-		PP_3060	0.84	+1.1
3,440,685	-		PP_3060	0.84	-1.7
3,440,685	-		PP_3060	0.84	-2.2
3,440,685	-		PP_3060	0.84	-0.3
3,440,766	-				-0.9
3,441,047	+		PP_3061	0.79	-0.7
3,441,047	+		PP_3061	0.79	-2.0
3,441,047	+		PP_3061	0.79	+0.4
3,441,056	+		PP_3061	0.82	-1.8
3,441,056	+		PP_3061	0.82	+0.3
3,441,056	+		PP_3061	0.82	-0.2
3,441,057	-		PP_3061	0.82	-0.8
3,441,107	+				-1.0
3,441,107	+				+0.8
3,441,107	+				+1.6
3,441,108	-				+0.5
3,441,264	-				-0.6
3,441,680	+		PP_3062	0.13	+0.1
3,441,718	+		PP_3062	0.15	+0.1
3,441,748	+		PP_3062	0.15	-1.1
3,441,748	+		PP_3062	0.15	+0.1
3,441,784	+		PP_3062	0.17	+0.4
3,441,784	+		PP_3062	0.17	-0.1
3,441,784	+		PP_3062	0.17	-0.8
3,441,784	+		PP_3062	0.17	+0.9
3,441,785	-		PP_3062	0.17	+0.5
3,441,785	-		PP_3062	0.17	-0.6
3,441,785	-		PP_3062	0.17	-1.1
3,441,785	-		PP_3062	0.17	-1.0
3,441,860	+		PP_3062	0.19	+0.4
3,441,861	-		PP_3062	0.19	-1.4
3,442,208	-		PP_3062	0.30	-1.3
3,442,221	-		PP_3062	0.30	+0.7
3,442,316	+		PP_3062	0.33	-0.0
3,442,509	-		PP_3062	0.39	-1.0
3,442,520	-		PP_3062	0.39	-1.3
3,442,768	-		PP_3062	0.47	+0.8
3,443,044	-		PP_3062	0.55	+0.8
3,443,060	-		PP_3062	0.56	-1.8
3,443,365	+		PP_3062	0.65	+1.1
3,443,365	+		PP_3062	0.65	-1.0
3,443,366	-		PP_3062	0.65	-0.8
3,443,699	+		PP_3062	0.75	+0.4
3,443,699	+		PP_3062	0.75	-1.2
3,443,700	-		PP_3062	0.75	-1.2
3,443,700	-		PP_3062	0.75	-0.4
3,443,700	-		PP_3062	0.75	+0.3
3,443,700	-		PP_3062	0.75	-0.4
3,443,921	+		PP_3062	0.82	+0.3
3,443,921	+		PP_3062	0.82	+1.3
3,443,921	+		PP_3062	0.82	-0.7
3,444,099	+		PP_3062	0.87	-0.2
3,444,100	-		PP_3062	0.87	-2.2
3,444,304	+				-0.2
3,444,304	+				+1.3
3,444,361	+				-1.4
3,444,388	+				-0.8
3,444,510	-				+0.6
3,444,520	+				-1.2
3,444,520	+				-1.1
3,444,521	-				+0.4
3,444,521	-				+0.3
3,444,521	-				-0.2
3,444,527	+				-1.8
3,444,527	+				-0.4
3,444,528	-				+0.6
3,444,676	-		PP_3063	0.18	-0.9
3,444,761	+		PP_3063	0.28	+1.2
3,444,761	+		PP_3063	0.28	+1.2
3,444,762	-		PP_3063	0.28	+1.9
3,444,762	-		PP_3063	0.28	+0.1
3,444,762	-		PP_3063	0.28	+0.2
3,444,996	-		PP_3063	0.56	+0.4
3,444,996	-		PP_3063	0.56	-0.2
3,444,998	+		PP_3063	0.56	-1.6
3,444,999	-		PP_3063	0.57	-1.0
3,445,154	-		PP_3063	0.75	-0.6
3,445,322	-				+1.2
3,445,341	-				-0.7
3,445,341	-				+0.9
3,445,341	-				-0.7
3,445,401	+		PP_3064	0.29	-0.3
3,445,402	-		PP_3064	0.29	-1.7
3,445,402	-		PP_3064	0.29	+0.1