Strain Fitness in Pseudomonas putida KT2440 around PP_3029

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
3,414,430	+		PP_3026	0.88	-0.5
3,414,454	+				-1.0
3,414,455	-				-0.4
3,414,476	+				+0.4
3,414,477	-				-0.2
3,414,490	-				-0.5
3,414,490	-				+1.0
3,414,490	-				-0.3
3,414,515	+				+0.5
3,414,515	+				-0.6
3,414,516	-				-0.1
3,414,516	-				-0.7
3,414,522	-				-1.8
3,414,621	+				-0.1
3,414,622	-				-1.2
3,414,641	+				+1.1
3,414,642	-				+0.6
3,414,642	-				+0.7
3,414,661	+				-0.0
3,414,662	-				+0.7
3,414,719	+				+1.8
3,414,842	-				-0.7
3,414,849	-				-0.0
3,414,849	-				+1.8
3,414,874	+				-2.7
3,414,897	+				+0.1
3,414,898	-				-1.4
3,414,898	-				+2.8
3,414,913	+		PP_3027	0.11	+0.3
3,414,914	-		PP_3027	0.11	+3.5
3,414,914	-		PP_3027	0.11	-1.2
3,414,959	+		PP_3027	0.32	+2.0
3,414,960	-		PP_3027	0.32	-0.1
3,414,960	-		PP_3027	0.32	+2.2
3,414,960	-		PP_3027	0.32	+1.5
3,414,960	-		PP_3027	0.32	-2.0
3,414,972	+		PP_3027	0.38	-1.1
3,414,972	+		PP_3027	0.38	+0.7
3,414,973	-		PP_3027	0.38	+0.2
3,414,973	-		PP_3027	0.38	-0.8
3,414,973	-		PP_3027	0.38	+0.1
3,414,973	-		PP_3027	0.38	-0.6
3,415,107	+				-0.0
3,415,138	+		PP_3028	0.12	-0.1
3,415,138	+		PP_3028	0.12	-1.2
3,415,138	+		PP_3028	0.12	+2.8
3,415,138	+		PP_3028	0.12	+0.3
3,415,139	-		PP_3028	0.12	-1.7
3,415,154	+		PP_3028	0.17	+0.6
3,415,154	+		PP_3028	0.17	+1.1
3,415,332	+		PP_3028	0.74	+0.3
3,415,332	+		PP_3028	0.74	-2.0
3,415,359	+		PP_3028	0.82	-0.8
3,415,360	-		PP_3028	0.83	+0.0
3,415,360	-		PP_3028	0.83	-1.0
3,415,360	-		PP_3028	0.83	-2.1
3,415,360	-		PP_3028	0.83	-0.0
3,415,389	+				+0.3
3,415,389	+				+2.6
3,415,389	+				+1.1
3,415,390	-				+0.1
3,415,524	+				-0.1
3,415,524	+				+0.1
3,415,524	+				-2.3
3,415,525	-				-0.7
3,415,525	-				-0.5
3,415,589	+				+2.3
3,415,589	+				+0.5
3,415,590	-				+0.8
3,415,590	-				-1.2
3,415,690	+		PP_3029	0.15	-3.1
3,415,715	+		PP_3029	0.17	+0.8
3,415,716	-		PP_3029	0.17	-2.9
3,415,874	+		PP_3029	0.25	-0.0
3,415,877	+		PP_3029	0.25	-0.4
3,415,878	-		PP_3029	0.26	-0.4
3,415,889	+		PP_3029	0.26	-2.5
3,415,890	-		PP_3029	0.26	+1.3
3,415,970	+		PP_3029	0.31	-0.8
3,415,970	+		PP_3029	0.31	+0.0
3,415,970	+		PP_3029	0.31	+2.2
3,415,971	-		PP_3029	0.31	-0.4
3,416,275	-		PP_3029	0.47	-1.3
3,416,377	-		PP_3029	0.53	-0.2
3,416,592	+		PP_3029	0.65	+0.3
3,416,592	+				+0.8
3,417,021	-		PP_3029	0.88	-1.1
3,417,021	-		PP_3029	0.88	-0.8
3,417,035	+		PP_3029	0.89	-1.0
3,417,035	+		PP_3029	0.89	+0.7
3,417,035	+		PP_3029	0.89	-2.4
3,417,036	-		PP_3029	0.89	+0.3
3,417,100	+				+0.6
3,417,116	+				+0.3
3,417,166	+				-0.2
3,417,166	+				-0.7
3,417,167	-				-1.6
3,417,167	-				+2.1
3,417,170	+				+0.7
3,417,170	+				-0.6
3,417,171	-				-1.7
3,417,171	-				+1.0
3,417,219	+				+0.5
3,417,220	-				-2.7
3,417,220	-				-0.6
3,417,275	+				-0.2
3,417,276	-				-0.2
3,417,276	-				-0.1
3,417,278	+				+0.9
3,417,278	+				-0.2
3,417,278	+				+0.6
3,417,278	+				-1.2
3,417,278	+				+2.6
3,417,279	-				-1.6
3,417,279	-				-2.5
3,417,279	-				+0.2
3,417,344	+		PP_3030	0.13	-1.2
3,417,345	-		PP_3030	0.13	+0.2
3,417,447	+		PP_3030	0.26	-1.5
3,417,457	+		PP_3030	0.27	-0.1
3,417,538	+		PP_3030	0.37	+0.4
3,417,538	+		PP_3030	0.37	+0.6
3,417,542	+		PP_3030	0.38	-1.1
3,417,543	-		PP_3030	0.38	-1.6
3,417,582	+		PP_3030	0.43	+0.7
3,417,582	+		PP_3030	0.43	+0.3
3,417,582	+		PP_3030	0.43	-2.1
3,417,582	+		PP_3030	0.43	-0.5
3,417,583	-		PP_3030	0.43	-1.4
3,417,583	-		PP_3030	0.43	+0.1
3,417,583	-		PP_3030	0.43	-0.8
3,417,583	-		PP_3030	0.43	-1.8
3,417,608	+		PP_3030	0.46	+0.1
3,417,609	-		PP_3030	0.46	+0.2
3,417,609	-		PP_3030	0.46	-0.4
3,417,651	+		PP_3030	0.51	-1.8
3,417,651	+		PP_3030	0.51	+1.0
3,417,652	-		PP_3030	0.52	+0.3
3,417,697	-		PP_3030	0.57	+1.4
3,417,731	+		PP_3030	0.61	-2.3
3,417,731	+		PP_3030	0.61	-0.4
3,417,732	-		PP_3030	0.62	+0.8
3,417,732	-		PP_3030	0.62	-0.2
3,417,873	+		PP_3030	0.79	-0.2
3,417,873	+		PP_3030	0.79	-1.3
3,417,933	+		PP_3030	0.87	-0.2
3,417,933	+		PP_3030	0.87	+2.0
3,417,933	+		PP_3030	0.87	-1.3
3,417,933	+		PP_3030	0.87	-2.0
3,417,976	+				-0.7
3,417,976	+				-2.0
3,417,977	-				+1.0
3,417,977	-				-0.0
3,417,982	+				-2.5
3,417,983	-				-2.6
3,417,983	-				-0.6
3,417,993	+				+0.4
3,417,993	+				+0.5
3,418,047	+				-0.7
3,418,047	+				-0.3
3,418,047	+				+0.2
3,418,048	-				-0.9
3,418,048	-				+1.1
3,418,087	+				+1.4
3,418,088	-				+2.0
3,418,095	+				+0.2
3,418,143	+		PP_3031	0.15	-0.9