Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2998 and PP_2999 overlap by 4 nucleotides PP_2999 and PP_3000 overlap by 4 nucleotides PP_3000 and PP_3001 overlap by 4 nucleotides PP_3001 and PP_3002 overlap by 4 nucleotides PP_3002 and PP_3003 are separated by 4 nucleotides
PP_2998: PP_2998 - 2-dehydropantoate 2-reductase, at 3,394,500 to 3,395,447
_2998
PP_2999: PP_2999 - Glyoxalase family protein, at 3,395,444 to 3,395,842
_2999
PP_3000: PP_3000 - MaoC domain protein, at 3,395,839 to 3,396,294
_3000
PP_3001: PP_3001 - CAIB/BAIF family protein, at 3,396,291 to 3,397,523
_3001
PP_3002: PP_3002 - shikimate dehydrogenase-related enzyme, at 3,397,520 to 3,398,338
_3002
PP_3003: PP_3003 - 3-dehydroquinate dehydratase 3, type II, at 3,398,343 to 3,398,780
_3003
Position (kb)
3396
3397
3398 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3395.402 kb on + strand at 3395.463 kb on - strand at 3395.580 kb on - strand, within PP_2999 at 3395.902 kb on + strand, within PP_3000 at 3395.902 kb on + strand, within PP_3000 at 3395.903 kb on - strand, within PP_3000 at 3395.903 kb on - strand, within PP_3000 at 3395.933 kb on - strand, within PP_3000 at 3395.981 kb on - strand, within PP_3000 at 3395.981 kb on - strand, within PP_3000 at 3396.287 kb on + strand at 3396.288 kb on - strand at 3396.334 kb on - strand at 3396.357 kb on + strand at 3396.357 kb on + strand at 3396.357 kb on + strand at 3396.586 kb on - strand, within PP_3001 at 3396.657 kb on + strand, within PP_3001 at 3396.658 kb on - strand, within PP_3001 at 3396.788 kb on + strand, within PP_3001 at 3396.807 kb on + strand at 3396.808 kb on - strand, within PP_3001 at 3396.808 kb on - strand, within PP_3001 at 3396.808 kb on - strand, within PP_3001 at 3396.808 kb on - strand, within PP_3001 at 3396.897 kb on + strand, within PP_3001 at 3396.898 kb on - strand, within PP_3001 at 3396.924 kb on + strand, within PP_3001 at 3396.939 kb on + strand, within PP_3001 at 3396.940 kb on - strand, within PP_3001 at 3396.940 kb on - strand, within PP_3001 at 3396.940 kb on - strand, within PP_3001 at 3397.006 kb on - strand, within PP_3001 at 3397.389 kb on - strand, within PP_3001 at 3397.405 kb on + strand at 3397.502 kb on + strand at 3397.502 kb on + strand at 3397.619 kb on + strand, within PP_3002 at 3397.662 kb on + strand, within PP_3002 at 3397.663 kb on - strand, within PP_3002 at 3398.153 kb on + strand, within PP_3002 at 3398.336 kb on + strand at 3398.356 kb on + strand at 3398.356 kb on + strand at 3398.356 kb on + strand at 3398.356 kb on + strand at 3398.356 kb on + strand at 3398.357 kb on - strand at 3398.357 kb on - strand at 3398.357 kb on - strand at 3398.413 kb on - strand, within PP_3003
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,395,402 + +1.3 3,395,463 - -2.0 3,395,580 - PP_2999 0.34 +0.5 3,395,902 + PP_3000 0.14 +2.1 3,395,902 + PP_3000 0.14 -0.6 3,395,903 - PP_3000 0.14 -0.3 3,395,903 - PP_3000 0.14 +0.1 3,395,933 - PP_3000 0.21 -0.3 3,395,981 - PP_3000 0.31 -1.3 3,395,981 - PP_3000 0.31 -1.9 3,396,287 + -3.1 3,396,288 - -2.2 3,396,334 - -0.7 3,396,357 + -0.3 3,396,357 + -0.4 3,396,357 + -0.7 3,396,586 - PP_3001 0.24 -2.7 3,396,657 + PP_3001 0.30 -0.6 3,396,658 - PP_3001 0.30 +0.1 3,396,788 + PP_3001 0.40 +0.1 3,396,807 + +2.2 3,396,808 - PP_3001 0.42 -1.7 3,396,808 - PP_3001 0.42 -1.3 3,396,808 - PP_3001 0.42 -0.9 3,396,808 - PP_3001 0.42 -0.2 3,396,897 + PP_3001 0.49 -0.8 3,396,898 - PP_3001 0.49 +1.0 3,396,924 + PP_3001 0.51 +0.4 3,396,939 + PP_3001 0.53 +1.2 3,396,940 - PP_3001 0.53 -0.3 3,396,940 - PP_3001 0.53 +0.6 3,396,940 - PP_3001 0.53 -2.6 3,397,006 - PP_3001 0.58 -1.1 3,397,389 - PP_3001 0.89 +0.0 3,397,405 + +1.0 3,397,502 + -1.7 3,397,502 + +0.8 3,397,619 + PP_3002 0.12 -0.0 3,397,662 + PP_3002 0.17 +1.0 3,397,663 - PP_3002 0.17 +0.6 3,398,153 + PP_3002 0.77 -0.7 3,398,336 + +0.4 3,398,356 + +0.6 3,398,356 + -0.5 3,398,356 + -0.0 3,398,356 + +0.3 3,398,356 + -2.1 3,398,357 - -1.3 3,398,357 - +0.1 3,398,357 - -2.2 3,398,413 - PP_3003 0.16 -1.0
Or see this region's nucleotide sequence