Strain Fitness in Pseudomonas putida KT2440 around PP_2992

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
3,388,688	-				+0.7
3,388,714	+				-1.6
3,388,714	+				+1.1
3,388,715	-				-0.3
3,388,720	+				+0.6
3,388,721	-				+2.0
3,388,757	-		PP_2991	0.11	+0.7
3,388,849	+		PP_2991	0.20	+2.0
3,388,850	-		PP_2991	0.20	+1.6
3,388,880	+		PP_2991	0.23	+0.8
3,388,981	+		PP_2991	0.32	+0.6
3,388,982	-		PP_2991	0.32	-1.9
3,389,030	+		PP_2991	0.37	-1.6
3,389,030	+		PP_2991	0.37	+0.9
3,389,042	-		PP_2991	0.38	+0.8
3,389,094	+		PP_2991	0.43	-0.4
3,389,094	+		PP_2991	0.43	+0.7
3,389,094	+		PP_2991	0.43	+0.9
3,389,095	-		PP_2991	0.43	-0.5
3,389,095	-		PP_2991	0.43	+0.9
3,389,095	-		PP_2991	0.43	-1.5
3,389,095	-		PP_2991	0.43	-0.7
3,389,098	+		PP_2991	0.44	+0.3
3,389,098	+		PP_2991	0.44	+0.2
3,389,098	+		PP_2991	0.44	+0.8
3,389,098	+		PP_2991	0.44	+0.2
3,389,099	-		PP_2991	0.44	+0.6
3,389,099	-		PP_2991	0.44	+0.3
3,389,099	-		PP_2991	0.44	-0.4
3,389,175	-		PP_2991	0.51	-0.3
3,389,224	+		PP_2991	0.56	-2.2
3,389,224	+		PP_2991	0.56	-0.9
3,389,224	+		PP_2991	0.56	-0.9
3,389,224	+		PP_2991	0.56	-0.8
3,389,225	-		PP_2991	0.56	-2.0
3,389,225	-		PP_2991	0.56	-0.1
3,389,225	-		PP_2991	0.56	+1.7
3,389,225	-		PP_2991	0.56	+0.3
3,389,225	-		PP_2991	0.56	+0.4
3,389,225	-		PP_2991	0.56	+0.9
3,389,230	+		PP_2991	0.56	+0.7
3,389,230	+		PP_2991	0.56	-1.6
3,389,230	+		PP_2991	0.56	+0.8
3,389,230	+		PP_2991	0.56	+0.1
3,389,231	-		PP_2991	0.57	+0.5
3,389,231	-		PP_2991	0.57	-1.2
3,389,231	-		PP_2991	0.57	+0.2
3,389,231	-		PP_2991	0.57	+0.1
3,389,237	+		PP_2991	0.57	-0.1
3,389,238	-		PP_2991	0.57	+0.3
3,389,289	+		PP_2991	0.62	-1.2
3,389,290	-		PP_2991	0.62	+1.2
3,389,292	+		PP_2991	0.62	+1.4
3,389,292	+		PP_2991	0.62	-0.9
3,389,369	-		PP_2991	0.70	-0.0
3,389,383	+		PP_2991	0.71	+1.1
3,389,628	+				+0.0
3,389,697	+				+0.9
3,389,698	-				-0.1
3,389,698	-				+0.4
3,389,701	-				-1.1
3,389,736	+				+1.5
3,390,019	+		PP_2992	0.41	+1.0
3,390,019	+		PP_2992	0.41	+0.6
3,390,019	+		PP_2992	0.41	+1.3
3,390,020	-		PP_2992	0.41	-1.0
3,390,054	-		PP_2992	0.46	-0.6
3,390,094	+		PP_2992	0.50	+0.8
3,390,094	+		PP_2992	0.50	+1.3
3,390,095	-		PP_2992	0.51	+0.0
3,390,123	-		PP_2992	0.54	-0.6
3,390,202	+		PP_2992	0.64	+0.1
3,390,202	+		PP_2992	0.64	+3.0
3,390,202	+		PP_2992	0.64	-0.8
3,390,202	+				+1.5
3,390,203	-		PP_2992	0.64	-0.1
3,390,272	-		PP_2992	0.73	+0.2
3,390,272	-		PP_2992	0.73	-0.4
3,390,316	-		PP_2992	0.78	-1.3
3,390,349	+		PP_2992	0.82	-1.5
3,390,368	+		PP_2992	0.85	+0.1
3,390,369	-		PP_2992	0.85	+0.6
3,390,450	+				+0.8
3,390,450	+				-1.3
3,390,450	+				+0.6
3,390,450	+				-2.0
3,390,451	-				+0.4
3,390,451	-				+0.4
3,390,457	-				+0.5
3,390,457	-				+1.4
3,390,532	+				+1.0
3,390,569	-				-2.0
3,390,569	-				+0.8
3,390,569	-				-0.4
3,390,730	+				-0.2
3,390,740	-				+0.7
3,390,757	+				+0.9
3,390,758	-				-2.0
3,390,918	-		PP_2994	0.10	+1.0
3,390,918	-		PP_2994	0.10	+0.9
3,390,952	+		PP_2994	0.13	-0.1
3,390,952	+		PP_2994	0.13	+1.0
3,390,952	+		PP_2994	0.13	+0.6
3,390,953	-		PP_2994	0.13	+0.4
3,391,009	-		PP_2994	0.17	-0.2
3,391,012	-		PP_2994	0.18	+1.5
3,391,012	-		PP_2994	0.18	+0.3
3,391,012	-		PP_2994	0.18	-0.9
3,391,092	+		PP_2994	0.24	-1.0
3,391,092	+		PP_2994	0.24	-1.4
3,391,231	+		PP_2994	0.35	+0.8
3,391,231	+		PP_2994	0.35	+1.2
3,391,232	-		PP_2994	0.35	+0.9