Strain Fitness in Pseudomonas putida KT2440 around PP_2988

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
3,385,432	-				-2.5
3,385,441	+				+1.4
3,385,674	-		PP_2987	0.13	+0.2
3,385,822	+		PP_2987	0.46	-0.8
3,385,822	+		PP_2987	0.46	+0.1
3,385,823	-		PP_2987	0.46	+0.5
3,385,867	-		PP_2987	0.56	-0.2
3,385,983	+		PP_2987	0.82	+0.0
3,385,984	-		PP_2987	0.83	-0.4
3,385,984	-		PP_2987	0.83	+0.3
3,385,984	-		PP_2987	0.83	-0.1
3,385,984	-		PP_2987	0.83	-2.4
3,386,094	-				+0.5
3,386,263	+		PP_2988	0.10	-1.9
3,386,263	+		PP_2988	0.10	+2.1
3,386,264	-		PP_2988	0.11	+0.3
3,386,335	+		PP_2988	0.18	-1.8
3,386,336	-		PP_2988	0.18	+1.7
3,386,345	-		PP_2988	0.18	+1.0
3,386,396	-		PP_2988	0.24	+0.7
3,386,396	-		PP_2988	0.24	-2.0
3,386,400	-		PP_2988	0.24	-0.2
3,386,699	+		PP_2988	0.53	+0.5
3,386,699	+		PP_2988	0.53	-0.1
3,386,700	-				+1.5
3,386,700	-		PP_2988	0.53	+0.8
3,386,705	+		PP_2988	0.54	+0.2
3,386,705	+		PP_2988	0.54	+1.1
3,386,705	+		PP_2988	0.54	-2.5
3,386,706	-		PP_2988	0.54	+1.5
3,386,706	-		PP_2988	0.54	+1.7
3,386,758	-		PP_2988	0.59	+0.2
3,386,793	+		PP_2988	0.63	+0.6
3,386,815	+		PP_2988	0.65	-0.9
3,386,816	-		PP_2988	0.65	+1.2
3,386,998	-		PP_2988	0.83	-0.0
3,387,126	+				-1.3
3,387,161	-				+0.4
3,387,161	-				+0.7
3,387,184	-				+0.9
3,387,206	+				+2.6
3,387,206	+				+0.7
3,387,206	+				-1.1
3,387,207	-				-0.1
3,387,207	-				-0.2
3,387,207	-				-1.2
3,387,212	+				+1.0
3,387,213	-				-0.3
3,387,213	-				+0.6
3,387,213	-				-1.1
3,387,213	-				-0.9
3,387,213	-				+0.5
3,387,239	+				-1.7
3,387,240	-				+0.7
3,387,246	+				+0.9
3,387,246	+				-0.6
3,387,277	+				+0.9
3,387,278	-				-2.1
3,387,356	+		PP_2989	0.17	+1.8
3,387,357	-		PP_2989	0.17	+0.3
3,387,357	-		PP_2989	0.17	-1.1
3,387,531	+		PP_2989	0.39	+0.1
3,387,532	-				+0.3
3,387,532	-		PP_2989	0.39	+0.7
3,387,676	+		PP_2989	0.57	+0.8
3,387,676	+		PP_2989	0.57	-1.0
3,387,677	-		PP_2989	0.57	+1.5
3,387,677	-		PP_2989	0.57	-0.7
3,387,748	+		PP_2989	0.66	+0.3
3,387,749	-		PP_2989	0.67	-0.6
3,387,890	+		PP_2989	0.84	-2.0
3,388,053	+				+0.7
3,388,053	+				+0.8
3,388,151	+				-0.6
3,388,151	+				-1.2
3,388,151	+				-1.8
3,388,152	-				-2.6
3,388,152	-				-1.4
3,388,160	-				-0.1
3,388,160	-				+2.3
3,388,160	-				-0.5