Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2949 and PP_2950 are separated by 112 nucleotides PP_2950 and PP_2951 are separated by 53 nucleotides PP_2951 and PP_2952 are separated by 125 nucleotides
PP_2949: PP_2949 - putative permease, at 3,353,756 to 3,354,622
_2949
PP_2950: PP_2950 - conserved protein of unknown function, at 3,354,735 to 3,355,211
_2950
PP_2951: PP_2951 - Transcriptional regulator, TetR family, at 3,355,265 to 3,355,831
_2951
PP_2952: PP_2952 - Transcriptional regulator, LysR family, at 3,355,957 to 3,356,847
_2952
Position (kb)
3354
3355
3356 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3353.794 kb on - strand at 3353.794 kb on - strand at 3353.923 kb on - strand, within PP_2949 at 3353.967 kb on + strand, within PP_2949 at 3353.968 kb on - strand, within PP_2949 at 3354.027 kb on + strand, within PP_2949 at 3354.027 kb on + strand, within PP_2949 at 3354.028 kb on - strand, within PP_2949 at 3354.028 kb on - strand, within PP_2949 at 3354.028 kb on - strand, within PP_2949 at 3354.028 kb on - strand, within PP_2949 at 3354.073 kb on + strand, within PP_2949 at 3354.245 kb on + strand, within PP_2949 at 3354.245 kb on + strand, within PP_2949 at 3354.378 kb on + strand, within PP_2949 at 3354.407 kb on + strand, within PP_2949 at 3354.620 kb on + strand at 3354.621 kb on - strand at 3354.654 kb on + strand at 3354.655 kb on - strand at 3354.655 kb on - strand at 3354.656 kb on + strand at 3354.657 kb on - strand at 3354.682 kb on + strand at 3354.682 kb on + strand at 3354.683 kb on - strand at 3354.683 kb on - strand at 3354.683 kb on - strand at 3354.683 kb on - strand at 3354.708 kb on - strand at 3354.708 kb on - strand at 3354.712 kb on - strand at 3354.712 kb on - strand at 3354.736 kb on + strand at 3354.938 kb on - strand, within PP_2950 at 3355.089 kb on - strand, within PP_2950 at 3355.089 kb on - strand, within PP_2950 at 3355.089 kb on - strand, within PP_2950 at 3355.090 kb on + strand, within PP_2950 at 3355.090 kb on + strand, within PP_2950 at 3355.252 kb on + strand at 3355.252 kb on + strand at 3355.253 kb on - strand at 3355.440 kb on - strand, within PP_2951 at 3355.529 kb on - strand, within PP_2951 at 3355.529 kb on - strand, within PP_2951 at 3355.653 kb on - strand, within PP_2951 at 3355.653 kb on - strand, within PP_2951 at 3355.653 kb on - strand, within PP_2951 at 3355.655 kb on - strand, within PP_2951 at 3355.683 kb on - strand, within PP_2951 at 3355.693 kb on + strand, within PP_2951 at 3355.783 kb on - strand at 3355.787 kb on - strand at 3355.838 kb on - strand at 3355.845 kb on + strand at 3355.846 kb on - strand at 3356.011 kb on + strand at 3356.141 kb on + strand at 3356.141 kb on + strand, within PP_2952 at 3356.141 kb on + strand, within PP_2952 at 3356.141 kb on + strand, within PP_2952 at 3356.141 kb on + strand, within PP_2952
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,353,794 - +1.1 3,353,794 - +1.4 3,353,923 - PP_2949 0.19 +0.1 3,353,967 + PP_2949 0.24 -0.3 3,353,968 - PP_2949 0.24 +0.2 3,354,027 + PP_2949 0.31 -1.1 3,354,027 + PP_2949 0.31 -0.3 3,354,028 - PP_2949 0.31 -0.6 3,354,028 - PP_2949 0.31 -0.2 3,354,028 - PP_2949 0.31 +0.3 3,354,028 - PP_2949 0.31 +1.8 3,354,073 + PP_2949 0.37 -0.8 3,354,245 + PP_2949 0.56 -2.1 3,354,245 + PP_2949 0.56 -2.0 3,354,378 + PP_2949 0.72 -0.6 3,354,407 + PP_2949 0.75 +1.2 3,354,620 + -0.3 3,354,621 - +2.3 3,354,654 + +1.3 3,354,655 - +0.9 3,354,655 - -2.4 3,354,656 + -1.3 3,354,657 - +1.7 3,354,682 + -0.4 3,354,682 + -0.4 3,354,683 - +0.4 3,354,683 - +1.9 3,354,683 - +0.1 3,354,683 - +0.7 3,354,708 - +1.0 3,354,708 - -0.8 3,354,712 - +0.7 3,354,712 - -2.0 3,354,736 + -0.6 3,354,938 - PP_2950 0.43 -1.2 3,355,089 - PP_2950 0.74 -1.1 3,355,089 - PP_2950 0.74 -0.1 3,355,089 - PP_2950 0.74 -1.0 3,355,090 + PP_2950 0.74 -0.2 3,355,090 + PP_2950 0.74 -0.8 3,355,252 + -0.2 3,355,252 + +1.4 3,355,253 - +0.1 3,355,440 - PP_2951 0.31 +0.6 3,355,529 - PP_2951 0.47 +0.5 3,355,529 - PP_2951 0.47 +0.5 3,355,653 - PP_2951 0.68 -0.8 3,355,653 - PP_2951 0.68 +1.3 3,355,653 - PP_2951 0.68 -1.8 3,355,655 - PP_2951 0.69 -2.5 3,355,683 - PP_2951 0.74 +0.2 3,355,693 + PP_2951 0.75 +1.4 3,355,783 - -0.9 3,355,787 - -0.1 3,355,838 - -2.5 3,355,845 + +2.4 3,355,846 - +0.4 3,356,011 + -0.8 3,356,141 + +0.3 3,356,141 + PP_2952 0.21 -1.8 3,356,141 + PP_2952 0.21 -0.2 3,356,141 + PP_2952 0.21 +0.3 3,356,141 + PP_2952 0.21 -0.5
Or see this region's nucleotide sequence