Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2922 and PP_2923 are separated by 62 nucleotides PP_2923 and PP_2924 overlap by 10 nucleotides PP_2924 and PP_2925 are separated by 114 nucleotides
PP_2922: PP_2922 - Urea amidolyase-related protein, at 3,322,001 to 3,322,972
_2922
PP_2923: PP_2923 - conserved protein of unknown function, at 3,323,035 to 3,323,892
_2923
PP_2924: PP_2924 - conserved protein of unknown function, at 3,323,883 to 3,325,133
_2924
PP_2925: PP_2925 - malate:quinone oxidoreductase, at 3,325,248 to 3,326,867
_2925
Position (kb)
3323
3324
3325
3326 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3323.240 kb on + strand, within PP_2923 at 3323.243 kb on + strand, within PP_2923 at 3323.244 kb on - strand, within PP_2923 at 3323.244 kb on - strand, within PP_2923 at 3323.244 kb on - strand, within PP_2923 at 3323.448 kb on - strand, within PP_2923 at 3323.974 kb on + strand at 3323.975 kb on - strand at 3324.041 kb on - strand, within PP_2924 at 3324.081 kb on + strand, within PP_2924 at 3324.081 kb on + strand, within PP_2924 at 3324.081 kb on + strand, within PP_2924 at 3324.081 kb on + strand, within PP_2924 at 3324.082 kb on - strand, within PP_2924 at 3324.082 kb on - strand, within PP_2924 at 3324.241 kb on + strand, within PP_2924 at 3324.241 kb on + strand, within PP_2924 at 3324.242 kb on - strand, within PP_2924 at 3324.274 kb on + strand, within PP_2924 at 3324.274 kb on + strand, within PP_2924 at 3324.355 kb on + strand, within PP_2924 at 3324.356 kb on - strand, within PP_2924 at 3324.356 kb on - strand, within PP_2924 at 3324.356 kb on - strand, within PP_2924 at 3324.356 kb on - strand, within PP_2924 at 3324.386 kb on - strand, within PP_2924 at 3324.413 kb on + strand, within PP_2924 at 3324.413 kb on + strand, within PP_2924 at 3324.414 kb on - strand, within PP_2924 at 3324.414 kb on - strand, within PP_2924 at 3324.642 kb on + strand, within PP_2924 at 3324.643 kb on - strand, within PP_2924 at 3324.685 kb on + strand, within PP_2924 at 3324.685 kb on + strand, within PP_2924 at 3324.685 kb on + strand, within PP_2924 at 3324.686 kb on - strand, within PP_2924 at 3324.748 kb on + strand, within PP_2924 at 3324.749 kb on - strand, within PP_2924 at 3324.925 kb on - strand, within PP_2924 at 3324.961 kb on + strand, within PP_2924 at 3324.961 kb on + strand, within PP_2924 at 3324.961 kb on + strand, within PP_2924 at 3324.962 kb on - strand, within PP_2924 at 3324.974 kb on + strand, within PP_2924 at 3324.975 kb on - strand, within PP_2924 at 3324.975 kb on - strand, within PP_2924 at 3325.057 kb on + strand at 3325.058 kb on - strand at 3325.247 kb on + strand at 3325.248 kb on - strand at 3325.376 kb on + strand at 3325.377 kb on - strand at 3325.377 kb on - strand at 3325.516 kb on - strand, within PP_2925 at 3325.711 kb on - strand, within PP_2925 at 3325.753 kb on - strand, within PP_2925 at 3325.885 kb on - strand, within PP_2925 at 3325.885 kb on - strand, within PP_2925 at 3326.100 kb on + strand, within PP_2925 at 3326.100 kb on + strand, within PP_2925 at 3326.100 kb on + strand, within PP_2925
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,323,240 + PP_2923 0.24 +1.5 3,323,243 + PP_2923 0.24 -0.5 3,323,244 - PP_2923 0.24 -2.5 3,323,244 - PP_2923 0.24 -0.5 3,323,244 - PP_2923 0.24 -1.4 3,323,448 - PP_2923 0.48 +0.3 3,323,974 + +0.8 3,323,975 - -1.5 3,324,041 - PP_2924 0.13 -3.3 3,324,081 + PP_2924 0.16 +1.5 3,324,081 + PP_2924 0.16 -0.4 3,324,081 + PP_2924 0.16 -0.6 3,324,081 + PP_2924 0.16 -0.2 3,324,082 - PP_2924 0.16 -0.2 3,324,082 - PP_2924 0.16 -0.1 3,324,241 + PP_2924 0.29 -1.5 3,324,241 + PP_2924 0.29 -0.3 3,324,242 - PP_2924 0.29 +0.3 3,324,274 + PP_2924 0.31 -0.4 3,324,274 + PP_2924 0.31 -1.3 3,324,355 + PP_2924 0.38 +1.2 3,324,356 - PP_2924 0.38 +0.2 3,324,356 - PP_2924 0.38 +0.2 3,324,356 - PP_2924 0.38 +1.3 3,324,356 - PP_2924 0.38 -1.7 3,324,386 - PP_2924 0.40 -0.1 3,324,413 + PP_2924 0.42 -2.3 3,324,413 + PP_2924 0.42 -0.7 3,324,414 - PP_2924 0.42 -1.4 3,324,414 - PP_2924 0.42 -1.1 3,324,642 + PP_2924 0.61 -0.4 3,324,643 - PP_2924 0.61 +0.1 3,324,685 + PP_2924 0.64 +1.0 3,324,685 + PP_2924 0.64 -1.5 3,324,685 + PP_2924 0.64 +0.5 3,324,686 - PP_2924 0.64 -0.0 3,324,748 + PP_2924 0.69 -1.1 3,324,749 - PP_2924 0.69 -1.2 3,324,925 - PP_2924 0.83 -1.7 3,324,961 + PP_2924 0.86 -0.1 3,324,961 + PP_2924 0.86 -0.1 3,324,961 + PP_2924 0.86 -1.9 3,324,962 - PP_2924 0.86 -0.3 3,324,974 + PP_2924 0.87 -1.3 3,324,975 - PP_2924 0.87 -0.6 3,324,975 - PP_2924 0.87 -0.7 3,325,057 + +0.6 3,325,058 - -0.2 3,325,247 + -0.4 3,325,248 - -1.0 3,325,376 + -2.8 3,325,377 - +1.9 3,325,377 - -0.5 3,325,516 - PP_2925 0.17 -0.5 3,325,711 - PP_2925 0.29 -0.3 3,325,753 - PP_2925 0.31 -0.8 3,325,885 - PP_2925 0.39 -0.3 3,325,885 - PP_2925 0.39 +0.7 3,326,100 + PP_2925 0.53 +0.6 3,326,100 + PP_2925 0.53 +1.4 3,326,100 + PP_2925 0.53 +0.0
Or see this region's nucleotide sequence