Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2906 and PP_2907 overlap by 23 nucleotides PP_2907 and PP_2908 are separated by 74 nucleotides PP_2908 and PP_2909 are separated by 195 nucleotides
PP_2906: PP_2906 - Sensor histidine kinase, at 3,304,779 to 3,306,170
_2906
PP_2907: PP_2907 - DNA-binding response regulator, at 3,306,148 to 3,306,828
_2907
PP_2908: PP_2908 - DNA-binding transcriptional repressor, at 3,306,903 to 3,307,598
_2908
PP_2909: PP_2909 - glutarate hydroxylase (from data) , at 3,307,794 to 3,308,771
_2909
Position (kb)
3306
3307 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3305.212 kb on - strand, within PP_2906 at 3305.601 kb on + strand, within PP_2906 at 3305.603 kb on + strand, within PP_2906 at 3305.604 kb on - strand, within PP_2906 at 3305.604 kb on - strand, within PP_2906 at 3305.696 kb on + strand, within PP_2906 at 3305.696 kb on + strand, within PP_2906 at 3305.697 kb on - strand, within PP_2906 at 3305.757 kb on + strand, within PP_2906 at 3305.757 kb on + strand, within PP_2906 at 3305.757 kb on + strand, within PP_2906 at 3306.044 kb on - strand at 3306.062 kb on - strand at 3306.132 kb on - strand at 3306.132 kb on - strand at 3306.149 kb on + strand at 3306.190 kb on - strand at 3306.360 kb on - strand, within PP_2907 at 3306.360 kb on - strand, within PP_2907 at 3306.463 kb on + strand, within PP_2907 at 3306.484 kb on + strand, within PP_2907 at 3306.484 kb on + strand, within PP_2907 at 3306.609 kb on + strand, within PP_2907 at 3306.846 kb on + strand at 3306.846 kb on + strand at 3306.846 kb on + strand at 3306.847 kb on - strand at 3306.904 kb on + strand at 3307.123 kb on + strand, within PP_2908 at 3307.124 kb on - strand at 3307.231 kb on - strand, within PP_2908 at 3307.231 kb on - strand, within PP_2908 at 3307.231 kb on - strand, within PP_2908 at 3307.253 kb on + strand, within PP_2908 at 3307.254 kb on - strand, within PP_2908 at 3307.254 kb on - strand, within PP_2908 at 3307.254 kb on - strand, within PP_2908 at 3307.348 kb on + strand, within PP_2908 at 3307.349 kb on - strand, within PP_2908 at 3307.474 kb on + strand, within PP_2908 at 3307.631 kb on - strand at 3307.673 kb on + strand at 3307.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,305,212 - PP_2906 0.31 -1.3 3,305,601 + PP_2906 0.59 -0.5 3,305,603 + PP_2906 0.59 +0.2 3,305,604 - PP_2906 0.59 -0.8 3,305,604 - PP_2906 0.59 -0.3 3,305,696 + PP_2906 0.66 +0.3 3,305,696 + PP_2906 0.66 -0.6 3,305,697 - PP_2906 0.66 -2.1 3,305,757 + PP_2906 0.70 -0.9 3,305,757 + PP_2906 0.70 +0.3 3,305,757 + PP_2906 0.70 -0.0 3,306,044 - -1.4 3,306,062 - +0.0 3,306,132 - -0.1 3,306,132 - +0.3 3,306,149 + -0.5 3,306,190 - +0.6 3,306,360 - PP_2907 0.31 +3.8 3,306,360 - PP_2907 0.31 +1.0 3,306,463 + PP_2907 0.46 +1.4 3,306,484 + PP_2907 0.49 -0.7 3,306,484 + PP_2907 0.49 -0.1 3,306,609 + PP_2907 0.68 -0.3 3,306,846 + -0.8 3,306,846 + +0.0 3,306,846 + +0.7 3,306,847 - +0.2 3,306,904 + +0.2 3,307,123 + PP_2908 0.32 -1.5 3,307,124 - +2.1 3,307,231 - PP_2908 0.47 -1.0 3,307,231 - PP_2908 0.47 -0.6 3,307,231 - PP_2908 0.47 -0.8 3,307,253 + PP_2908 0.50 -0.1 3,307,254 - PP_2908 0.50 +0.8 3,307,254 - PP_2908 0.50 +0.3 3,307,254 - PP_2908 0.50 +0.9 3,307,348 + PP_2908 0.64 -0.4 3,307,349 - PP_2908 0.64 -1.5 3,307,474 + PP_2908 0.82 -0.1 3,307,631 - -1.2 3,307,673 + +1.4 3,307,673 + -0.2
Or see this region's nucleotide sequence