Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2834 and PP_2835 are separated by 59 nucleotides PP_2835 and PP_2836 are separated by 20 nucleotides
PP_2834: PP_2834 - Putative D-galactarate dehydratase/Altronate dehydratase, at 3,234,906 to 3,236,447
_2834
PP_2835: PP_2835 - putative oxidoreductase, at 3,236,507 to 3,237,541
_2835
PP_2836: PP_2836 - putative 2-keto-3-deoxyxylonate dehydratase, at 3,237,562 to 3,238,779
_2836
Position (kb)
3236
3237
3238 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3235.604 kb on + strand, within PP_2834 at 3235.605 kb on - strand, within PP_2834 at 3235.821 kb on - strand, within PP_2834 at 3235.851 kb on + strand, within PP_2834 at 3235.851 kb on + strand, within PP_2834 at 3235.852 kb on - strand, within PP_2834 at 3235.949 kb on + strand, within PP_2834 at 3236.010 kb on - strand, within PP_2834 at 3236.070 kb on + strand, within PP_2834 at 3236.070 kb on + strand, within PP_2834 at 3236.070 kb on + strand, within PP_2834 at 3236.205 kb on + strand at 3236.502 kb on - strand at 3236.519 kb on - strand at 3236.577 kb on + strand at 3236.578 kb on - strand at 3236.595 kb on + strand at 3236.596 kb on - strand at 3236.596 kb on - strand at 3236.652 kb on + strand, within PP_2835 at 3236.745 kb on - strand at 3236.759 kb on + strand, within PP_2835 at 3236.826 kb on + strand, within PP_2835 at 3236.865 kb on - strand, within PP_2835 at 3237.391 kb on - strand, within PP_2835 at 3237.522 kb on + strand at 3237.700 kb on + strand, within PP_2836 at 3237.700 kb on + strand, within PP_2836 at 3237.700 kb on + strand, within PP_2836 at 3237.935 kb on + strand, within PP_2836 at 3237.936 kb on - strand, within PP_2836 at 3238.443 kb on + strand, within PP_2836 at 3238.443 kb on + strand, within PP_2836
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,235,604 + PP_2834 0.45 +0.5 3,235,605 - PP_2834 0.45 -0.6 3,235,821 - PP_2834 0.59 -2.2 3,235,851 + PP_2834 0.61 -0.1 3,235,851 + PP_2834 0.61 +0.0 3,235,852 - PP_2834 0.61 -0.5 3,235,949 + PP_2834 0.68 -0.6 3,236,010 - PP_2834 0.72 +0.3 3,236,070 + PP_2834 0.75 -1.9 3,236,070 + PP_2834 0.75 -0.7 3,236,070 + PP_2834 0.75 -1.8 3,236,205 + +2.7 3,236,502 - +1.9 3,236,519 - +0.2 3,236,577 + +0.2 3,236,578 - +0.8 3,236,595 + +0.4 3,236,596 - +2.1 3,236,596 - -1.6 3,236,652 + PP_2835 0.14 +0.3 3,236,745 - +2.7 3,236,759 + PP_2835 0.24 +0.4 3,236,826 + PP_2835 0.31 -2.1 3,236,865 - PP_2835 0.35 -1.6 3,237,391 - PP_2835 0.85 -1.6 3,237,522 + -1.4 3,237,700 + PP_2836 0.11 +0.6 3,237,700 + PP_2836 0.11 +1.0 3,237,700 + PP_2836 0.11 -0.2 3,237,935 + PP_2836 0.31 +0.8 3,237,936 - PP_2836 0.31 -0.2 3,238,443 + PP_2836 0.72 +0.8 3,238,443 + PP_2836 0.72 +0.6
Or see this region's nucleotide sequence