Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2802 and PP_2803 are separated by 12 nucleotides PP_2803 and PP_2804 are separated by 191 nucleotides
PP_2802: PP_2802 - Amino acid permease family protein, at 3,193,538 to 3,194,869
_2802
PP_2803: PP_2803 - 1,3-propanediol dehydrogenase, at 3,194,882 to 3,196,066
_2803
PP_2804: PP_2804 - Hydrolase, alpha/beta fold family, at 3,196,258 to 3,197,133
_2804
Position (kb)
3194
3195
3196
3197 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3194.121 kb on + strand, within PP_2802 at 3194.121 kb on + strand, within PP_2802 at 3194.136 kb on + strand, within PP_2802 at 3194.353 kb on - strand, within PP_2802 at 3194.353 kb on - strand, within PP_2802 at 3194.353 kb on - strand, within PP_2802 at 3194.628 kb on + strand, within PP_2802 at 3194.629 kb on - strand, within PP_2802 at 3194.635 kb on - strand, within PP_2802 at 3194.677 kb on - strand, within PP_2802 at 3194.677 kb on - strand, within PP_2802 at 3194.718 kb on + strand, within PP_2802 at 3194.763 kb on + strand at 3194.884 kb on - strand at 3194.884 kb on - strand at 3194.898 kb on + strand at 3194.899 kb on - strand at 3194.930 kb on + strand at 3195.225 kb on + strand, within PP_2803 at 3195.225 kb on + strand, within PP_2803 at 3195.226 kb on - strand, within PP_2803 at 3195.226 kb on - strand, within PP_2803 at 3195.435 kb on + strand, within PP_2803 at 3195.436 kb on - strand, within PP_2803 at 3195.436 kb on - strand, within PP_2803 at 3195.547 kb on - strand, within PP_2803 at 3195.685 kb on - strand, within PP_2803 at 3195.685 kb on - strand at 3195.685 kb on - strand, within PP_2803 at 3196.023 kb on - strand at 3196.039 kb on + strand at 3196.039 kb on + strand at 3196.039 kb on + strand at 3196.043 kb on + strand at 3196.043 kb on + strand at 3196.044 kb on - strand at 3196.044 kb on - strand at 3196.044 kb on - strand at 3196.110 kb on - strand at 3196.125 kb on + strand at 3196.125 kb on + strand at 3196.126 kb on - strand at 3196.126 kb on - strand at 3196.674 kb on + strand, within PP_2804 at 3196.674 kb on + strand, within PP_2804 at 3196.675 kb on - strand, within PP_2804 at 3196.675 kb on - strand, within PP_2804 at 3196.869 kb on - strand, within PP_2804 at 3197.016 kb on - strand, within PP_2804
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,194,121 + PP_2802 0.44 -0.5 3,194,121 + PP_2802 0.44 -1.5 3,194,136 + PP_2802 0.45 +1.3 3,194,353 - PP_2802 0.61 +0.5 3,194,353 - PP_2802 0.61 +0.5 3,194,353 - PP_2802 0.61 +0.6 3,194,628 + PP_2802 0.82 +1.1 3,194,629 - PP_2802 0.82 -1.2 3,194,635 - PP_2802 0.82 -0.1 3,194,677 - PP_2802 0.86 -0.0 3,194,677 - PP_2802 0.86 -2.1 3,194,718 + PP_2802 0.89 +0.9 3,194,763 + +0.8 3,194,884 - +0.2 3,194,884 - -2.1 3,194,898 + -0.6 3,194,899 - +0.3 3,194,930 + -2.3 3,195,225 + PP_2803 0.29 -1.2 3,195,225 + PP_2803 0.29 -0.7 3,195,226 - PP_2803 0.29 -2.0 3,195,226 - PP_2803 0.29 +1.0 3,195,435 + PP_2803 0.47 -0.9 3,195,436 - PP_2803 0.47 -0.3 3,195,436 - PP_2803 0.47 -1.2 3,195,547 - PP_2803 0.56 +0.6 3,195,685 - PP_2803 0.68 -0.7 3,195,685 - +1.0 3,195,685 - PP_2803 0.68 +0.4 3,196,023 - +1.2 3,196,039 + +0.9 3,196,039 + -0.5 3,196,039 + +0.4 3,196,043 + +1.4 3,196,043 + -0.3 3,196,044 - -0.0 3,196,044 - -0.3 3,196,044 - +0.3 3,196,110 - -2.1 3,196,125 + +0.7 3,196,125 + +2.3 3,196,126 - +0.6 3,196,126 - +1.7 3,196,674 + PP_2804 0.47 -0.1 3,196,674 + PP_2804 0.47 -0.7 3,196,675 - PP_2804 0.48 +0.8 3,196,675 - PP_2804 0.48 -1.2 3,196,869 - PP_2804 0.70 -1.6 3,197,016 - PP_2804 0.87 -1.2
Or see this region's nucleotide sequence