Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2793 and PP_2794 overlap by 1 nucleotides PP_2794 and PP_2795 are separated by 83 nucleotides
PP_2793: PP_2793 - Acyl-CoA dehydrogenase family protein, at 3,182,341 to 3,183,513
_2793
PP_2794: PP_2794 - Oxidoreductase, short chain dehydrogenase/reductase family, at 3,183,513 to 3,184,280
_2794
PP_2795: PP_2795 - AMP-binding domain protein, at 3,184,364 to 3,186,010
_2795
Position (kb)
3183
3184
3185 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3182.617 kb on - strand, within PP_2793 at 3183.009 kb on + strand, within PP_2793 at 3183.056 kb on - strand, within PP_2793 at 3183.439 kb on + strand at 3183.439 kb on + strand at 3183.820 kb on + strand, within PP_2794 at 3183.988 kb on - strand, within PP_2794 at 3184.084 kb on - strand, within PP_2794 at 3184.278 kb on + strand at 3184.297 kb on - strand at 3184.315 kb on + strand at 3184.319 kb on + strand at 3184.319 kb on + strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.460 kb on + strand at 3184.484 kb on + strand at 3184.485 kb on - strand at 3184.722 kb on + strand, within PP_2795 at 3184.850 kb on + strand, within PP_2795 at 3184.851 kb on - strand, within PP_2795 at 3184.932 kb on - strand, within PP_2795 at 3184.932 kb on - strand, within PP_2795 at 3184.932 kb on - strand, within PP_2795 at 3184.989 kb on - strand, within PP_2795 at 3185.086 kb on + strand, within PP_2795
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,182,617 - PP_2793 0.24 -1.0 3,183,009 + PP_2793 0.57 -0.1 3,183,056 - PP_2793 0.61 +0.7 3,183,439 + -1.7 3,183,439 + +1.8 3,183,820 + PP_2794 0.40 +1.2 3,183,988 - PP_2794 0.62 -2.1 3,184,084 - PP_2794 0.74 -2.0 3,184,278 + +0.5 3,184,297 - -0.5 3,184,315 + -0.9 3,184,319 + +0.5 3,184,319 + -1.1 3,184,320 - -1.9 3,184,320 - +1.0 3,184,320 - -0.4 3,184,320 - +1.9 3,184,320 - +0.8 3,184,320 - -1.0 3,184,320 - +0.3 3,184,320 - -1.7 3,184,320 - -0.7 3,184,460 + -2.4 3,184,484 + +0.4 3,184,485 - +0.9 3,184,722 + PP_2795 0.22 -0.4 3,184,850 + PP_2795 0.30 +1.0 3,184,851 - PP_2795 0.30 +0.8 3,184,932 - PP_2795 0.34 -0.9 3,184,932 - PP_2795 0.34 +0.1 3,184,932 - PP_2795 0.34 +0.9 3,184,989 - PP_2795 0.38 +3.1 3,185,086 + PP_2795 0.44 +1.3
Or see this region's nucleotide sequence