Strain Fitness in Pseudomonas putida KT2440 around PP_2745

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
3,126,955	-		PP_2744	0.51	-0.1
3,127,110	-		PP_2744	0.67	-1.2
3,127,110	-		PP_2744	0.67	-0.0
3,127,110	-		PP_2744	0.67	+0.2
3,127,110	-		PP_2744	0.67	-1.2
3,127,110	-		PP_2744	0.67	-1.0
3,127,110	-		PP_2744	0.67	+1.4
3,127,126	+		PP_2744	0.69	-0.7
3,127,127	-		PP_2744	0.69	-2.4
3,127,136	-		PP_2744	0.70	-1.8
3,127,206	+		PP_2744	0.77	-1.0
3,127,206	+		PP_2744	0.77	-0.9
3,127,206	+		PP_2744	0.77	-0.1
3,127,207	-		PP_2744	0.77	-0.4
3,127,207	-		PP_2744	0.77	-2.3
3,127,241	-		PP_2744	0.81	+0.5
3,127,375	+				+0.3
3,127,488	+				+0.6
3,127,492	+				+0.9
3,127,573	+				-2.8
3,127,573	+				+0.0
3,127,573	+				+0.9
3,127,609	-				+0.2
3,127,654	-		PP_2745	0.15	-1.3
3,127,807	-		PP_2745	0.32	+1.1
3,127,833	+		PP_2745	0.35	+0.4
3,127,955	+		PP_2745	0.48	+0.4
3,128,019	-		PP_2745	0.56	+2.4
3,128,410	+				-2.0
3,128,410	+				-1.2
3,128,411	-				-0.7
3,128,411	-				+0.3
3,128,500	+				-2.1
3,128,501	-				+0.6
3,128,504	+				-0.5
3,128,529	-				-0.2
3,128,592	+				-0.7
3,128,592	+				+1.4
3,128,673	+				-1.2
3,128,850	+				+0.5
3,128,940	+		PP_2746	0.13	+0.5
3,128,941	-		PP_2746	0.13	+0.5
3,128,941	-		PP_2746	0.13	+0.9
3,129,158	+		PP_2746	0.29	-0.6
3,129,158	+		PP_2746	0.29	-1.2
3,129,158	+		PP_2746	0.29	+0.0
3,129,166	-		PP_2746	0.29	-0.6
3,129,231	+		PP_2746	0.34	-2.0
3,129,244	-		PP_2746	0.35	+1.3
3,129,258	+		PP_2746	0.36	-1.6
3,129,259	-		PP_2746	0.36	-0.2