Strain Fitness in Pseudomonas putida KT2440 around PP_2718

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
3,106,467	+		PP_2717	0.31	-2.0
3,106,479	+		PP_2717	0.32	+1.1
3,106,480	-		PP_2717	0.32	+0.7
3,106,480	-		PP_2717	0.32	+0.7
3,106,480	-		PP_2717	0.32	+0.6
3,106,731	+				+3.7
3,106,766	+		PP_2717	0.55	+1.0
3,107,034	-		PP_2717	0.76	+0.7
3,107,051	+		PP_2717	0.77	+2.0
3,107,202	-		PP_2717	0.89	+0.8
3,107,229	-				-1.4
3,107,229	-				-1.2
3,107,309	+				+0.7
3,107,309	+				-1.8
3,107,514	+		PP_2718	0.42	-1.0
3,107,515	-		PP_2718	0.42	-1.8
3,107,515	-		PP_2718	0.42	-0.2
3,107,768	-				+0.5
3,107,875	+				+0.9
3,107,875	+				-1.5
3,107,875	+				-0.1
3,107,876	-				+0.7
3,107,877	+				-0.6
3,107,878	-				-0.2
3,107,878	-				-0.1
3,107,941	+		PP_2719	0.37	-0.1
3,108,253	-		PP_2720	0.78	-3.0
3,108,337	-		PP_2721	0.16	+0.1
3,108,390	+		PP_2721	0.31	-2.2
3,108,487	+		PP_2721	0.59	-1.4
3,108,487	+		PP_2721	0.59	+1.5
3,108,488	-		PP_2721	0.59	-0.5
3,108,501	+		PP_2721	0.63	-0.5
3,108,502	-		PP_2721	0.63	-2.5
3,108,550	-		PP_2721	0.77	-1.9