Strain Fitness in Pseudomonas putida KT2440 around PP_2611

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,983,575	+		PP_2609	0.31	-0.6
2,983,575	+		PP_2609	0.31	-0.8
2,983,575	+		PP_2609	0.31	+0.4
2,983,575	+		PP_2609	0.31	-0.8
2,983,575	+		PP_2609	0.31	-0.1
2,983,575	+		PP_2609	0.31	-1.0
2,983,576	-		PP_2609	0.31	-0.4
2,983,576	-		PP_2609	0.31	+0.9
2,983,704	+		PP_2609	0.48	+0.8
2,983,706	+		PP_2609	0.48	-2.1
2,983,706	+		PP_2609	0.48	-0.5
2,983,707	-		PP_2609	0.48	+1.0
2,983,755	+		PP_2609	0.54	+1.7
2,983,755	+		PP_2609	0.54	+0.2
2,983,755	+		PP_2609	0.54	-1.1
2,983,756	-		PP_2609	0.55	+0.5
2,983,815	+		PP_2609	0.62	-0.5
2,983,815	+		PP_2609	0.62	+0.6
2,983,888	+		PP_2609	0.72	-0.4
2,983,889	-		PP_2609	0.72	-1.5
2,983,889	-		PP_2609	0.72	+0.1
2,983,889	-		PP_2609	0.72	+0.8
2,983,916	+		PP_2609	0.75	+0.1
2,983,916	+		PP_2609	0.75	-2.8
2,983,917	-		PP_2609	0.75	-0.2
2,983,917	-		PP_2609	0.75	-0.2
2,984,009	+		PP_2609	0.87	-0.7
2,984,009	+		PP_2609	0.87	+0.6
2,984,010	-		PP_2609	0.88	-0.3
2,984,010	-		PP_2609	0.88	+1.4
2,984,010	-		PP_2609	0.88	-0.3
2,984,030	+				-0.4
2,984,030	+				-0.8
2,984,056	+				+0.3
2,984,057	-				-0.6
2,984,057	-				+1.3
2,984,067	+				-0.0
2,984,068	-				+0.9
2,984,068	-				-2.1
2,984,309	+		PP_2610	0.21	+0.6
2,984,309	+		PP_2610	0.21	+0.8
2,984,310	-		PP_2610	0.21	+0.7
2,984,310	-		PP_2610	0.21	+1.2
2,984,480	+		PP_2610	0.87	-1.3
2,984,481	-		PP_2610	0.87	+0.1
2,984,481	-		PP_2610	0.87	+1.0
2,984,481	-		PP_2610	0.87	-0.3
2,984,481	-		PP_2610	0.87	-0.3
2,984,481	-		PP_2610	0.87	+0.3
2,984,493	+				-0.3
2,984,499	+				+1.2
2,984,499	+				+0.1
2,984,500	-				-0.0
2,984,955	+		PP_2611	0.50	+0.6
2,984,955	+		PP_2611	0.50	+0.9
2,984,955	+		PP_2611	0.50	-1.7
2,985,053	-		PP_2611	0.61	-0.1
2,985,055	-		PP_2611	0.61	-1.0
2,985,230	+		PP_2611	0.81	+0.0
2,985,311	+		PP_2611	0.90	-1.0
2,985,469	+				+0.4
2,985,470	-				+0.2
2,985,484	-				+0.0
2,985,507	-				+0.6
2,985,531	+				-0.6
2,985,548	+				+2.8
2,985,606	+				+1.3
2,985,657	-				-2.4
2,985,657	-				-1.6
2,985,660	+				+0.3
2,985,683	+				+0.0
2,985,684	-				+0.6
2,985,684	-				+0.5
2,985,696	-				-1.2
2,985,738	+		PP_2612	0.11	-1.8
2,985,740	+		PP_2612	0.11	+0.6
2,985,785	-		PP_2612	0.13	-1.6
2,985,888	+		PP_2612	0.16	+0.7
2,985,900	+		PP_2612	0.16	-1.0
2,985,955	-		PP_2612	0.18	-2.0
2,986,120	-		PP_2612	0.24	+0.1
2,986,128	-		PP_2612	0.24	+0.0
2,986,152	+		PP_2612	0.25	+0.6
2,986,260	-		PP_2612	0.28	-0.9
2,986,260	-		PP_2612	0.28	-0.3