Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2605 and PP_2606 are separated by 41 nucleotides PP_2606 and PP_2607 overlap by 16 nucleotides PP_2607 and PP_2608 are separated by 4 nucleotides PP_2608 and PP_2609 are separated by 156 nucleotides
PP_2605: PP_2605 - conserved protein of unknown function, at 2,978,596 to 2,980,284
_2605
PP_2606: PP_2606 - conserved protein of unknown function, at 2,980,326 to 2,980,646
_2606
PP_2607: PP_2607 - conserved exported protein of unknown function, at 2,980,631 to 2,982,364
_2607
PP_2608: PP_2608 - Shikimate dehydrogenase family protein, at 2,982,369 to 2,983,178
_2608
PP_2609: PP_2609 - Transcriptional regulator, IclR family, at 2,983,335 to 2,984,105
_2609
Position (kb)
2980
2981
2982
2983 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2979.838 kb on + strand, within PP_2605 at 2979.881 kb on - strand, within PP_2605 at 2979.939 kb on + strand at 2980.262 kb on - strand at 2980.478 kb on - strand, within PP_2606 at 2980.625 kb on - strand at 2980.625 kb on - strand at 2980.639 kb on + strand at 2980.639 kb on + strand at 2980.640 kb on - strand at 2980.642 kb on + strand at 2980.642 kb on + strand at 2980.642 kb on + strand at 2980.643 kb on - strand at 2980.797 kb on + strand at 2980.797 kb on + strand at 2980.798 kb on - strand at 2980.903 kb on - strand, within PP_2607 at 2980.903 kb on - strand, within PP_2607 at 2980.945 kb on + strand, within PP_2607 at 2980.945 kb on + strand, within PP_2607 at 2980.966 kb on - strand, within PP_2607 at 2980.966 kb on - strand, within PP_2607 at 2980.974 kb on + strand, within PP_2607 at 2981.115 kb on + strand, within PP_2607 at 2981.115 kb on + strand, within PP_2607 at 2981.256 kb on + strand, within PP_2607 at 2981.372 kb on + strand, within PP_2607 at 2981.373 kb on - strand, within PP_2607 at 2981.373 kb on - strand, within PP_2607 at 2981.606 kb on + strand, within PP_2607 at 2982.189 kb on + strand, within PP_2607 at 2982.190 kb on - strand, within PP_2607 at 2982.190 kb on - strand, within PP_2607 at 2982.459 kb on + strand, within PP_2608 at 2982.460 kb on - strand, within PP_2608 at 2982.650 kb on + strand, within PP_2608 at 2982.685 kb on - strand, within PP_2608 at 2982.723 kb on + strand, within PP_2608 at 2982.724 kb on - strand, within PP_2608 at 2982.754 kb on + strand, within PP_2608 at 2982.754 kb on + strand, within PP_2608 at 2982.897 kb on + strand, within PP_2608 at 2982.898 kb on - strand, within PP_2608 at 2982.898 kb on - strand, within PP_2608 at 2982.971 kb on - strand, within PP_2608 at 2982.971 kb on - strand, within PP_2608 at 2982.971 kb on - strand, within PP_2608 at 2982.971 kb on - strand, within PP_2608 at 2982.971 kb on - strand, within PP_2608 at 2983.190 kb on + strand at 2983.191 kb on - strand at 2983.191 kb on - strand at 2983.202 kb on + strand at 2983.203 kb on - strand at 2983.259 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,979,838 + PP_2605 0.74 +0.7 2,979,881 - PP_2605 0.76 +0.8 2,979,939 + +4.8 2,980,262 - +0.8 2,980,478 - PP_2606 0.47 +0.3 2,980,625 - -0.3 2,980,625 - +0.4 2,980,639 + -0.2 2,980,639 + +1.1 2,980,640 - -0.3 2,980,642 + -1.3 2,980,642 + -0.4 2,980,642 + -0.7 2,980,643 - -1.0 2,980,797 + +0.2 2,980,797 + -0.8 2,980,798 - -0.8 2,980,903 - PP_2607 0.16 +0.7 2,980,903 - PP_2607 0.16 +0.7 2,980,945 + PP_2607 0.18 -0.3 2,980,945 + PP_2607 0.18 -0.5 2,980,966 - PP_2607 0.19 +0.2 2,980,966 - PP_2607 0.19 +0.1 2,980,974 + PP_2607 0.20 -0.6 2,981,115 + PP_2607 0.28 -1.3 2,981,115 + PP_2607 0.28 -1.0 2,981,256 + PP_2607 0.36 -0.7 2,981,372 + PP_2607 0.43 -1.8 2,981,373 - PP_2607 0.43 -0.6 2,981,373 - PP_2607 0.43 -0.8 2,981,606 + PP_2607 0.56 -0.5 2,982,189 + PP_2607 0.90 -0.6 2,982,190 - PP_2607 0.90 -0.3 2,982,190 - PP_2607 0.90 +0.8 2,982,459 + PP_2608 0.11 -1.0 2,982,460 - PP_2608 0.11 -0.0 2,982,650 + PP_2608 0.35 -0.7 2,982,685 - PP_2608 0.39 -2.0 2,982,723 + PP_2608 0.44 +1.1 2,982,724 - PP_2608 0.44 -2.4 2,982,754 + PP_2608 0.48 +0.9 2,982,754 + PP_2608 0.48 -1.8 2,982,897 + PP_2608 0.65 +0.7 2,982,898 - PP_2608 0.65 -0.6 2,982,898 - PP_2608 0.65 -0.8 2,982,971 - PP_2608 0.74 -0.5 2,982,971 - PP_2608 0.74 -0.1 2,982,971 - PP_2608 0.74 -1.8 2,982,971 - PP_2608 0.74 +0.5 2,982,971 - PP_2608 0.74 -0.3 2,983,190 + -0.3 2,983,191 - -1.8 2,983,191 - -1.7 2,983,202 + +0.5 2,983,203 - -0.8 2,983,259 - -1.2
Or see this region's nucleotide sequence