Strain Fitness in Pseudomonas putida KT2440 around PP_2605

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,977,681	+		PP_2604	0.49	+1.0
2,977,682	-		PP_2604	0.49	-0.4
2,977,726	+		PP_2604	0.53	+1.1
2,977,726	+		PP_2604	0.53	+0.8
2,977,726	+		PP_2604	0.53	+0.8
2,977,729	+				+1.0
2,977,730	-		PP_2604	0.53	-0.4
2,977,730	-		PP_2604	0.53	+0.3
2,977,937	-		PP_2604	0.68	-0.3
2,978,037	+		PP_2604	0.76	+1.3
2,978,037	+		PP_2604	0.76	-0.1
2,978,110	+		PP_2604	0.82	+0.6
2,978,111	-		PP_2604	0.82	+0.8
2,978,216	-		PP_2604	0.90	+0.9
2,978,216	-		PP_2604	0.90	+1.7
2,978,216	-		PP_2604	0.90	+1.9
2,978,216	-		PP_2604	0.90	-0.1
2,978,354	+				-1.9
2,978,354	+				-0.2
2,978,355	-				-0.2
2,978,355	-				-0.4
2,978,355	-				+0.4
2,978,355	-				+1.4
2,978,355	-				+0.5
2,978,378	+				+1.3
2,978,394	+				+0.5
2,978,533	+				-1.0
2,978,552	+				+0.9
2,978,640	-				-1.5
2,978,667	+				-0.8
2,978,684	+				+0.6
2,978,688	-				-2.1
2,978,868	+		PP_2605	0.16	-0.3
2,978,868	+		PP_2605	0.16	-0.7
2,978,868	+				+1.2
2,978,869	-		PP_2605	0.16	-2.0
2,978,869	-		PP_2605	0.16	-0.3
2,978,872	+		PP_2605	0.16	-0.0
2,978,903	+		PP_2605	0.18	-0.7
2,978,903	+		PP_2605	0.18	-0.7
2,978,928	+		PP_2605	0.20	-0.1
2,978,929	-		PP_2605	0.20	-1.3
2,979,044	+		PP_2605	0.27	+0.7
2,979,059	+		PP_2605	0.27	-0.8
2,979,060	-		PP_2605	0.27	-0.7
2,979,104	+		PP_2605	0.30	-1.0
2,979,105	-		PP_2605	0.30	+1.1
2,979,198	-		PP_2605	0.36	-1.4
2,979,425	+		PP_2605	0.49	-0.5
2,979,468	+		PP_2605	0.52	+0.0
2,979,468	+		PP_2605	0.52	-0.5
2,979,524	+		PP_2605	0.55	-1.5
2,979,524	+		PP_2605	0.55	-1.5
2,979,525	-		PP_2605	0.55	+0.0
2,979,838	+		PP_2605	0.74	+0.7
2,979,881	-		PP_2605	0.76	+0.8
2,979,939	+				+4.8
2,980,262	-				+0.8
2,980,478	-		PP_2606	0.47	+0.3
2,980,625	-				-0.3
2,980,625	-				+0.4
2,980,639	+				-0.2
2,980,639	+				+1.1
2,980,640	-				-0.3
2,980,642	+				-1.3
2,980,642	+				-0.4
2,980,642	+				-0.7
2,980,643	-				-1.0
2,980,797	+				+0.2
2,980,797	+				-0.8
2,980,798	-				-0.8
2,980,903	-		PP_2607	0.16	+0.7
2,980,903	-		PP_2607	0.16	+0.7
2,980,945	+		PP_2607	0.18	-0.3
2,980,945	+		PP_2607	0.18	-0.5
2,980,966	-		PP_2607	0.19	+0.2
2,980,966	-		PP_2607	0.19	+0.1
2,980,974	+		PP_2607	0.20	-0.6
2,981,115	+		PP_2607	0.28	-1.3
2,981,115	+		PP_2607	0.28	-1.0
2,981,256	+		PP_2607	0.36	-0.7