Strain Fitness in Pseudomonas putida KT2440 around PP_2597

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,967,535	+		PP_2596	0.51	-2.0
2,967,535	+		PP_2596	0.51	+0.9
2,967,536	-		PP_2596	0.51	-1.6
2,967,536	-		PP_2596	0.51	-0.9
2,967,542	-		PP_2596	0.51	-0.7
2,968,489	+				+0.7
2,968,490	-				+1.0
2,968,670	+		PP_2597	0.24	-0.6
2,968,671	-		PP_2597	0.24	-1.6
2,968,671	-		PP_2597	0.24	-1.8
2,968,671	-		PP_2597	0.24	-0.2
2,968,781	+		PP_2597	0.42	-0.0
2,968,844	+		PP_2597	0.52	-1.8
2,968,845	-		PP_2597	0.53	+0.5
2,968,845	-		PP_2597	0.53	-1.1
2,968,845	-		PP_2597	0.53	+0.2
2,968,845	-		PP_2597	0.53	-0.2
2,969,000	+		PP_2597	0.78	+1.5
2,969,001	-		PP_2597	0.78	-0.4
2,969,143	+				+0.8
2,969,143	+				+0.7
2,969,143	+				+0.2
2,969,161	+				+0.1
2,969,162	-				-0.5
2,969,206	+				-1.1
2,969,206	+				-1.3
2,969,206	+				+0.5
2,969,206	+				-1.2
2,969,207	-				+0.5
2,969,207	-				+0.6
2,969,213	+				+0.5
2,969,213	+				+0.1
2,969,214	-				-2.2
2,969,214	-				-0.2
2,969,332	+				-0.1
2,969,689	+				+0.0
2,969,695	+				-0.3
2,969,735	+				-0.4
2,969,736	-				+0.5
2,969,745	+				+0.6
2,969,764	+				-0.7
2,969,764	+				-0.1
2,969,765	-				-0.6
2,969,765	-				+0.7
2,969,765	-				-0.3
2,969,811	+				-0.1
2,969,811	+				+0.0
2,970,034	+		PP_2599	0.16	-0.7