Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2525 and PP_2526 are separated by 146 nucleotides PP_2526 and PP_2527 are separated by 81 nucleotides PP_2527 and PP_2528 are separated by 64 nucleotides
PP_2525: PP_2525 - conserved protein of unknown function, at 2,871,911 to 2,873,074
_2525
PP_2526: PP_2526 - conserved membrane protein of unknown function, at 2,873,221 to 2,873,694
_2526
PP_2527: PP_2527 - conserved membrane protein of unknown function, at 2,873,776 to 2,874,540
_2527
PP_2528: PP_2528 - O-acetylhomoserine (thiol)-lyase, at 2,874,605 to 2,875,882
_2528
Position (kb)
2873
2874
2875 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2873.046 kb on + strand at 2873.065 kb on + strand at 2873.066 kb on - strand at 2873.183 kb on + strand at 2873.183 kb on + strand at 2873.183 kb on + strand at 2873.184 kb on - strand at 2873.185 kb on + strand at 2873.186 kb on - strand at 2873.186 kb on - strand at 2873.448 kb on - strand, within PP_2526 at 2873.596 kb on + strand, within PP_2526 at 2873.596 kb on + strand, within PP_2526 at 2873.605 kb on + strand, within PP_2526 at 2873.665 kb on + strand at 2873.666 kb on - strand at 2873.666 kb on - strand at 2873.666 kb on - strand at 2873.891 kb on + strand, within PP_2527 at 2873.891 kb on + strand, within PP_2527 at 2874.086 kb on - strand, within PP_2527 at 2874.086 kb on - strand, within PP_2527 at 2874.135 kb on + strand, within PP_2527 at 2874.209 kb on + strand, within PP_2527 at 2874.210 kb on - strand, within PP_2527 at 2874.210 kb on - strand, within PP_2527 at 2874.251 kb on + strand, within PP_2527 at 2874.251 kb on + strand, within PP_2527 at 2874.252 kb on - strand, within PP_2527 at 2874.252 kb on - strand, within PP_2527 at 2874.320 kb on + strand, within PP_2527 at 2874.321 kb on - strand, within PP_2527 at 2874.321 kb on - strand, within PP_2527 at 2874.528 kb on - strand at 2874.797 kb on + strand, within PP_2528 at 2874.972 kb on + strand, within PP_2528 at 2874.972 kb on + strand, within PP_2528 at 2874.973 kb on - strand, within PP_2528 at 2874.987 kb on - strand, within PP_2528 at 2875.158 kb on + strand, within PP_2528 at 2875.269 kb on + strand, within PP_2528 at 2875.269 kb on + strand at 2875.408 kb on + strand, within PP_2528 at 2875.409 kb on - strand at 2875.442 kb on + strand, within PP_2528 at 2875.443 kb on - strand, within PP_2528
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,873,046 + -0.6 2,873,065 + -0.6 2,873,066 - -0.7 2,873,183 + +1.0 2,873,183 + +0.4 2,873,183 + +0.9 2,873,184 - +0.3 2,873,185 + -2.3 2,873,186 - +1.6 2,873,186 - +0.0 2,873,448 - PP_2526 0.48 -2.5 2,873,596 + PP_2526 0.79 -0.6 2,873,596 + PP_2526 0.79 -1.5 2,873,605 + PP_2526 0.81 -0.3 2,873,665 + +0.7 2,873,666 - -1.4 2,873,666 - -0.7 2,873,666 - +1.8 2,873,891 + PP_2527 0.15 +0.5 2,873,891 + PP_2527 0.15 +0.7 2,874,086 - PP_2527 0.41 -1.8 2,874,086 - PP_2527 0.41 -0.4 2,874,135 + PP_2527 0.47 +0.1 2,874,209 + PP_2527 0.57 +0.3 2,874,210 - PP_2527 0.57 -1.2 2,874,210 - PP_2527 0.57 +1.7 2,874,251 + PP_2527 0.62 -0.8 2,874,251 + PP_2527 0.62 +0.3 2,874,252 - PP_2527 0.62 +2.1 2,874,252 - PP_2527 0.62 +1.6 2,874,320 + PP_2527 0.71 +1.7 2,874,321 - PP_2527 0.71 -1.1 2,874,321 - PP_2527 0.71 -1.0 2,874,528 - -0.9 2,874,797 + PP_2528 0.15 -1.2 2,874,972 + PP_2528 0.29 -0.3 2,874,972 + PP_2528 0.29 -2.5 2,874,973 - PP_2528 0.29 +0.2 2,874,987 - PP_2528 0.30 +1.7 2,875,158 + PP_2528 0.43 +1.1 2,875,269 + PP_2528 0.52 +1.8 2,875,269 + +0.1 2,875,408 + PP_2528 0.63 +0.5 2,875,409 - +1.9 2,875,442 + PP_2528 0.65 -1.3 2,875,443 - PP_2528 0.66 +1.1
Or see this region's nucleotide sequence