Strain Fitness in Pseudomonas putida KT2440 around PP_2495

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,842,910	+		PP_2494	0.32	-0.5
2,842,986	+		PP_2494	0.39	-2.0
2,843,042	-		PP_2494	0.45	-0.3
2,843,042	-		PP_2494	0.45	+1.2
2,843,042	-		PP_2494	0.45	-2.2
2,843,107	-		PP_2494	0.51	-0.3
2,843,146	+		PP_2494	0.55	+1.3
2,843,293	+		PP_2494	0.70	-1.0
2,843,294	-		PP_2494	0.70	-1.0
2,843,679	+				-2.0
2,843,764	-		PP_2495	0.13	+0.5
2,843,883	+		PP_2495	0.25	+1.1
2,843,883	+		PP_2495	0.25	-1.1
2,844,013	-		PP_2495	0.38	+0.7
2,844,107	+		PP_2495	0.47	-1.1
2,844,108	-		PP_2495	0.47	-0.1
2,844,113	+		PP_2495	0.47	+1.0
2,844,113	+		PP_2495	0.47	-0.4
2,844,113	+		PP_2495	0.47	+0.4
2,844,114	-		PP_2495	0.48	-1.9
2,844,114	-		PP_2495	0.48	-0.3
2,844,114	-		PP_2495	0.48	-0.4
2,844,172	-		PP_2495	0.53	+0.5
2,844,172	-		PP_2495	0.53	-1.4
2,844,325	+		PP_2495	0.69	-2.1
2,844,326	-		PP_2495	0.69	-1.1
2,844,343	+		PP_2495	0.70	+0.8
2,844,343	+		PP_2495	0.70	-0.8
2,844,344	-		PP_2495	0.70	+0.4
2,844,344	-		PP_2495	0.70	+0.2
2,844,344	-		PP_2495	0.70	-0.1
2,844,344	-		PP_2495	0.70	-0.7
2,844,357	+		PP_2495	0.72	-1.7
2,844,357	+		PP_2495	0.72	-1.8
2,844,358	-		PP_2495	0.72	+0.8
2,844,358	-		PP_2495	0.72	-0.3
2,844,396	+		PP_2495	0.76	-1.4
2,844,396	+		PP_2495	0.76	+0.7
2,844,396	+		PP_2495	0.76	+0.2
2,844,396	+		PP_2495	0.76	+0.1
2,844,400	+		PP_2495	0.76	-1.0
2,844,400	+		PP_2495	0.76	+1.3
2,844,400	+		PP_2495	0.76	-0.8
2,844,401	-		PP_2495	0.76	-0.6
2,844,401	-		PP_2495	0.76	+0.8
2,844,401	-		PP_2495	0.76	-2.7
2,844,401	-		PP_2495	0.76	+1.6
2,844,401	-		PP_2495	0.76	+0.8
2,844,401	-		PP_2495	0.76	-1.1
2,844,401	-		PP_2495	0.76	-1.3
2,844,401	-		PP_2495	0.76	-0.6
2,844,484	+		PP_2495	0.84	-1.4
2,844,484	+		PP_2495	0.84	+0.1
2,844,484	+		PP_2495	0.84	-0.1
2,844,485	-		PP_2495	0.84	-1.7
2,844,485	-		PP_2495	0.84	-1.1
2,844,486	+		PP_2495	0.85	-0.1
2,844,640	+				+1.5
2,844,640	+				+1.1
2,844,640	+				+0.8
2,844,641	-				-0.6
2,844,641	-				-1.8
2,844,641	-				-1.5
2,844,714	-				+0.9
2,844,743	+				-0.4
2,845,064	-		PP_2496	0.33	-0.2
2,845,064	-		PP_2496	0.33	-0.8
2,845,268	-		PP_2496	0.53	+0.4
2,845,268	-		PP_2496	0.53	-1.5
2,845,268	-		PP_2496	0.53	+1.1
2,845,268	-		PP_2496	0.53	+0.9
2,845,578	-		PP_2496	0.83	+1.0