Strain Fitness in Pseudomonas putida KT2440 around PP_2441

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,788,505	+		PP_2440	0.53	+0.8
2,788,506	-		PP_2440	0.53	+0.3
2,788,506	-		PP_2440	0.53	-0.6
2,788,506	-		PP_2440	0.53	+1.6
2,788,512	+		PP_2440	0.53	+1.4
2,788,702	-		PP_2440	0.66	+0.8
2,788,702	-				+3.3
2,789,141	+				+0.7
2,789,142	-				-0.1
2,789,151	+				-1.4
2,789,238	+				-2.0
2,789,238	+				-0.0
2,789,316	-				-1.1
2,789,373	+				+2.5
2,789,374	-				-1.1
2,789,374	-				+1.6
2,789,374	-				+0.5
2,789,374	-				+0.0
2,789,439	-		PP_2441	0.17	-0.9
2,789,439	-		PP_2441	0.17	-1.0
2,789,439	-		PP_2441	0.17	+2.1
2,789,766	+		PP_2442	0.26	+0.2
2,789,767	-		PP_2442	0.26	-1.1
2,790,284	-				-0.3
2,790,284	-				-0.4
2,790,284	-				-1.6
2,790,287	+				+1.3
2,790,287	+				-1.8
2,790,287	+				-2.0
2,790,288	-				+1.0
2,790,288	-				+0.7
2,790,288	-				-1.9
2,790,288	-				+0.4
2,790,288	-				-1.2
2,790,288	-				-0.6
2,790,288	-				+0.7
2,790,288	-				+1.4
2,790,300	+				-0.1
2,790,301	-				+0.6
2,790,337	+				-0.8