Strain Fitness in Pseudomonas putida KT2440 around PP_2440

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,786,766	+				-0.6
2,786,766	+				+1.0
2,786,766	+				+0.1
2,786,767	-				+1.5
2,786,767	-				-0.6
2,786,864	-				-2.3
2,786,880	+				-0.6
2,786,880	+				-0.5
2,786,883	-				+0.4
2,786,937	+				+1.0
2,786,938	-				+0.7
2,786,941	+				+1.0
2,786,942	-				+0.5
2,786,949	+				+0.8
2,786,949	+				+0.6
2,786,949	+				-0.0
2,787,035	+				+0.8
2,787,036	-				+2.3
2,787,161	+		PP_2439	0.30	+0.6
2,787,162	-		PP_2439	0.30	-0.1
2,787,162	-		PP_2439	0.30	+0.3
2,787,195	-		PP_2439	0.36	-0.9
2,787,196	-		PP_2439	0.37	-0.5
2,787,270	-		PP_2439	0.50	-0.9
2,787,411	+		PP_2439	0.75	-1.2
2,787,411	+		PP_2439	0.75	+1.6
2,787,411	+		PP_2439	0.75	+0.1
2,787,412	-		PP_2439	0.75	-0.9
2,787,439	+		PP_2439	0.80	-0.2
2,787,439	+		PP_2439	0.80	+0.2
2,787,458	+		PP_2439	0.83	-1.1
2,787,458	+		PP_2439	0.83	-1.3
2,787,540	+				+0.4
2,787,551	+				+0.5
2,787,551	+				+0.4
2,787,551	+				-0.4
2,787,551	+				-1.3
2,787,552	-				+0.6
2,787,552	-				-0.8
2,787,552	-				+1.0
2,787,552	-				+0.6
2,787,577	+				-1.9
2,787,577	+				+1.0
2,787,599	+				+0.1
2,787,600	-				+0.7
2,787,603	+				-1.7
2,787,604	-				-0.6
2,787,677	+				-0.8
2,787,678	-				+1.1
2,787,696	-				+0.0
2,787,827	+				-1.6
2,787,827	+				+0.5
2,787,828	-				+0.5
2,788,105	+		PP_2440	0.27	+0.6
2,788,193	+		PP_2440	0.33	+1.0
2,788,193	+		PP_2440	0.33	+0.1
2,788,194	-		PP_2440	0.33	-1.2
2,788,197	+		PP_2440	0.33	+0.1
2,788,197	+		PP_2440	0.33	-0.9
2,788,229	+		PP_2440	0.35	+0.4
2,788,362	+		PP_2440	0.44	+1.7
2,788,363	-		PP_2440	0.44	+0.6
2,788,363	-		PP_2440	0.44	-0.4
2,788,363	-		PP_2440	0.44	+0.1
2,788,505	+		PP_2440	0.53	+0.8
2,788,506	-		PP_2440	0.53	+0.3
2,788,506	-		PP_2440	0.53	-0.6
2,788,506	-		PP_2440	0.53	+1.6
2,788,512	+		PP_2440	0.53	+1.4
2,788,702	-		PP_2440	0.66	+0.8
2,788,702	-				+3.3
2,789,141	+				+0.7
2,789,142	-				-0.1
2,789,151	+				-1.4
2,789,238	+				-2.0
2,789,238	+				-0.0
2,789,316	-				-1.1
2,789,373	+				+2.5
2,789,374	-				-1.1
2,789,374	-				+1.6
2,789,374	-				+0.5
2,789,374	-				+0.0
2,789,439	-		PP_2441	0.17	-0.9
2,789,439	-		PP_2441	0.17	-1.0
2,789,439	-		PP_2441	0.17	+2.1
2,789,766	+		PP_2442	0.26	+0.2
2,789,767	-		PP_2442	0.26	-1.1