Strain Fitness in Pseudomonas putida KT2440 around PP_2412

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,757,408	-		PP_2411	0.41	-0.3
2,757,422	+		PP_2411	0.42	-1.0
2,757,423	-		PP_2411	0.43	-1.3
2,757,423	-		PP_2411	0.43	-0.5
2,757,579	-		PP_2411	0.54	-0.1
2,757,773	+		PP_2411	0.69	-1.1
2,757,791	+		PP_2411	0.70	-0.8
2,757,869	+		PP_2411	0.76	+0.2
2,757,869	+		PP_2411	0.76	-0.7
2,757,870	-		PP_2411	0.76	+0.9
2,758,095	-				+1.2
2,758,095	-				-0.2
2,758,148	-				-0.9
2,758,258	-				-0.1
2,758,258	-				+0.1
2,758,272	+				-1.2
2,758,273	-				-0.4
2,758,318	-				+0.2
2,758,319	+				-0.6
2,758,319	+				-0.4
2,758,320	-				-1.8
2,758,320	-				-0.1
2,758,346	-				-1.1
2,758,426	-				-0.4
2,758,499	+		PP_2412	0.16	-0.6
2,758,585	+		PP_2412	0.27	-1.3
2,758,733	+		PP_2412	0.46	-2.1
2,758,734	-		PP_2412	0.46	+1.2
2,758,775	+		PP_2412	0.51	+0.3
2,758,775	+		PP_2412	0.51	-1.1
2,758,775	+		PP_2412	0.51	-0.6
2,758,776	-		PP_2412	0.51	+0.3
2,758,776	-		PP_2412	0.51	-0.0
2,758,893	-		PP_2412	0.66	-0.6
2,759,013	-		PP_2412	0.81	+0.5
2,759,168	+				+1.2
2,759,195	-				+1.2
2,759,259	-				+0.3
2,759,293	+				+0.0
2,759,293	+				-0.7
2,759,471	-				+0.3
2,759,618	-		PP_2413	0.19	-0.6
2,759,861	+		PP_2413	0.34	-0.9
2,759,862	-		PP_2413	0.34	-1.7