Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2399 and PP_2400 are separated by 34 nucleotides PP_2400 and PP_2401 are separated by 14 nucleotides PP_2401 and PP_2402 are separated by 169 nucleotides
PP_2399: PP_2399 - conserved protein of unknown function, at 2,744,462 to 2,745,157
_2399
PP_2400: PP_2400 - conserved protein of unknown function, at 2,745,192 to 2,745,680
_2400
PP_2401: PP_2401 - conserved membrane protein of unknown function, at 2,745,695 to 2,746,312
_2401
PP_2402: PP_2402 - putative Sensor histidine kinase, at 2,746,482 to 2,747,768
_2402
Position (kb)
2745
2746
2747 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2744.786 kb on + strand, within PP_2399 at 2744.787 kb on - strand, within PP_2399 at 2744.810 kb on + strand, within PP_2399 at 2744.811 kb on - strand, within PP_2399 at 2744.811 kb on - strand, within PP_2399 at 2744.904 kb on - strand, within PP_2399 at 2745.025 kb on + strand, within PP_2399 at 2745.025 kb on + strand, within PP_2399 at 2745.026 kb on - strand, within PP_2399 at 2745.026 kb on - strand, within PP_2399 at 2745.026 kb on - strand, within PP_2399 at 2745.277 kb on + strand, within PP_2400 at 2745.278 kb on - strand, within PP_2400 at 2745.296 kb on - strand, within PP_2400 at 2745.325 kb on + strand, within PP_2400 at 2745.325 kb on + strand, within PP_2400 at 2745.326 kb on - strand, within PP_2400 at 2745.326 kb on - strand, within PP_2400 at 2745.387 kb on + strand, within PP_2400 at 2745.459 kb on + strand, within PP_2400 at 2745.459 kb on + strand, within PP_2400 at 2745.949 kb on - strand, within PP_2401 at 2746.026 kb on + strand, within PP_2401 at 2746.026 kb on + strand, within PP_2401 at 2746.027 kb on - strand, within PP_2401 at 2746.077 kb on + strand, within PP_2401 at 2746.077 kb on + strand, within PP_2401 at 2746.077 kb on + strand, within PP_2401 at 2746.078 kb on - strand, within PP_2401 at 2746.175 kb on - strand, within PP_2401 at 2746.235 kb on + strand, within PP_2401 at 2746.236 kb on - strand, within PP_2401 at 2746.236 kb on - strand, within PP_2401 at 2746.363 kb on + strand at 2746.364 kb on - strand at 2746.395 kb on + strand at 2746.396 kb on - strand at 2746.396 kb on - strand at 2746.396 kb on - strand at 2746.396 kb on - strand at 2746.396 kb on - strand at 2746.706 kb on + strand, within PP_2402 at 2746.706 kb on + strand at 2746.707 kb on - strand, within PP_2402 at 2746.707 kb on - strand, within PP_2402 at 2746.870 kb on - strand, within PP_2402 at 2746.943 kb on - strand, within PP_2402 at 2747.274 kb on - strand, within PP_2402 at 2747.274 kb on - strand, within PP_2402
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,744,786 + PP_2399 0.47 +0.5 2,744,787 - PP_2399 0.47 -0.1 2,744,810 + PP_2399 0.50 +1.6 2,744,811 - PP_2399 0.50 -0.9 2,744,811 - PP_2399 0.50 +0.5 2,744,904 - PP_2399 0.64 -0.0 2,745,025 + PP_2399 0.81 +0.6 2,745,025 + PP_2399 0.81 -1.2 2,745,026 - PP_2399 0.81 -0.2 2,745,026 - PP_2399 0.81 +0.6 2,745,026 - PP_2399 0.81 +1.1 2,745,277 + PP_2400 0.17 -1.7 2,745,278 - PP_2400 0.18 +0.5 2,745,296 - PP_2400 0.21 -0.7 2,745,325 + PP_2400 0.27 +0.4 2,745,325 + PP_2400 0.27 +0.5 2,745,326 - PP_2400 0.27 +0.4 2,745,326 - PP_2400 0.27 +1.1 2,745,387 + PP_2400 0.40 -2.5 2,745,459 + PP_2400 0.55 -1.9 2,745,459 + PP_2400 0.55 -1.2 2,745,949 - PP_2401 0.41 -0.9 2,746,026 + PP_2401 0.54 +1.2 2,746,026 + PP_2401 0.54 +0.0 2,746,027 - PP_2401 0.54 -0.2 2,746,077 + PP_2401 0.62 +0.9 2,746,077 + PP_2401 0.62 +0.1 2,746,077 + PP_2401 0.62 +0.4 2,746,078 - PP_2401 0.62 +0.9 2,746,175 - PP_2401 0.78 +0.9 2,746,235 + PP_2401 0.87 +0.2 2,746,236 - PP_2401 0.88 +1.8 2,746,236 - PP_2401 0.88 +1.3 2,746,363 + +0.4 2,746,364 - -0.6 2,746,395 + -0.4 2,746,396 - -0.9 2,746,396 - -1.1 2,746,396 - +0.4 2,746,396 - -0.7 2,746,396 - -1.1 2,746,706 + PP_2402 0.17 +0.1 2,746,706 + +0.6 2,746,707 - PP_2402 0.17 +0.7 2,746,707 - PP_2402 0.17 +1.2 2,746,870 - PP_2402 0.30 -0.1 2,746,943 - PP_2402 0.36 +1.9 2,747,274 - PP_2402 0.62 +3.6 2,747,274 - PP_2402 0.62 +0.6
Or see this region's nucleotide sequence