Strain Fitness in Pseudomonas putida KT2440 around PP_2385

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,723,469	+		PP_2383	0.85	+1.4
2,723,469	+		PP_2383	0.85	+0.4
2,723,469	+		PP_2383	0.85	-1.8
2,723,469	+		PP_2383	0.85	+0.0
2,723,470	-		PP_2383	0.86	-0.6
2,723,470	-		PP_2383	0.86	+0.4
2,723,470	-		PP_2383	0.86	+0.2
2,723,470	-		PP_2383	0.86	+0.5
2,723,473	+		PP_2383	0.86	+0.6
2,723,474	-		PP_2383	0.86	-1.8
2,723,474	-		PP_2383	0.86	-1.5
2,723,509	+				+2.4
2,723,509	+				-0.1
2,723,510	-				+0.1
2,723,512	+				+0.5
2,723,512	+				+0.3
2,723,513	-				+1.1
2,723,648	+		PP_2384	0.19	+0.9
2,723,648	+		PP_2384	0.19	-1.8
2,723,648	+		PP_2384	0.19	-0.0
2,723,648	+		PP_2384	0.19	-2.0
2,723,682	-		PP_2384	0.30	-1.0
2,723,682	-		PP_2384	0.30	-0.8
2,723,682	-		PP_2384	0.30	-1.1
2,723,700	+		PP_2384	0.36	+0.5
2,723,850	+				+0.3
2,723,991	-		PP_2385	0.13	-1.9
2,723,991	-		PP_2385	0.13	-0.5
2,723,991	-		PP_2385	0.13	-1.5
2,724,176	+		PP_2385	0.40	-0.8
2,724,176	+		PP_2385	0.40	-1.8
2,724,177	-		PP_2385	0.40	-0.3
2,724,177	-		PP_2385	0.40	-1.5
2,724,231	-		PP_2385	0.48	-0.7
2,724,310	+		PP_2385	0.59	-0.4
2,724,332	+		PP_2385	0.62	+1.5
2,724,332	+		PP_2385	0.62	-1.4
2,724,332	+		PP_2385	0.62	-0.9
2,724,333	-		PP_2385	0.63	-2.3
2,724,333	-		PP_2385	0.63	+0.8
2,724,609	+				-0.7
2,724,740	+				-0.4
2,724,790	+				+0.6
2,724,790	+				-1.2
2,724,791	-				+0.1
2,724,791	-				-0.5
2,724,849	-		PP_2386	0.13	-2.0
2,724,863	+		PP_2386	0.15	-0.2
2,724,945	-		PP_2386	0.25	-1.0
2,724,947	+		PP_2386	0.25	-0.3
2,724,948	-		PP_2386	0.25	-1.7
2,724,948	-		PP_2386	0.25	-1.0
2,724,956	+		PP_2386	0.26	-0.6
2,724,956	+		PP_2386	0.26	-0.1
2,724,993	+		PP_2386	0.31	-0.5
2,724,993	+		PP_2386	0.31	-0.3
2,724,993	+		PP_2386	0.31	-0.4
2,724,993	+		PP_2386	0.31	+0.1
2,724,993	+		PP_2386	0.31	-1.0
2,724,994	-		PP_2386	0.31	-0.7
2,724,994	-		PP_2386	0.31	+0.4
2,724,994	-		PP_2386	0.31	-0.4
2,724,999	-		PP_2386	0.31	-0.8
2,725,124	+		PP_2386	0.46	-0.7
2,725,124	+		PP_2386	0.46	-0.4
2,725,125	-		PP_2386	0.46	-1.5
2,725,133	+				+0.1
2,725,133	+		PP_2386	0.47	-0.6
2,725,134	-		PP_2386	0.48	+0.1
2,725,134	-		PP_2386	0.48	-0.7
2,725,134	-		PP_2386	0.48	+1.8
2,725,134	-		PP_2386	0.48	-0.2
2,725,134	-		PP_2386	0.48	+0.7
2,725,136	+		PP_2386	0.48	+0.4
2,725,334	+		PP_2386	0.72	-0.9
2,725,364	-		PP_2386	0.75	-0.5
2,725,459	+		PP_2386	0.87	+0.1
2,725,460	-		PP_2386	0.87	-0.5
2,725,460	-		PP_2386	0.87	-2.7
2,725,516	-				+1.3
2,725,526	+				+0.1
2,725,526	+				-0.1
2,725,527	-				-1.6
2,725,527	-				-0.9
2,725,527	-				-0.7
2,725,527	-				-0.6
2,725,531	+				-1.1