Strain Fitness in Pseudomonas putida KT2440 around PP_2369

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,701,993	+		PP_2368	0.20	-1.4
2,701,993	+		PP_2368	0.20	+1.1
2,702,036	+		PP_2368	0.25	+0.5
2,702,037	-		PP_2368	0.25	-0.3
2,702,093	-		PP_2368	0.32	-1.2
2,702,107	+		PP_2368	0.34	+0.4
2,702,107	+		PP_2368	0.34	-0.2
2,702,107	+		PP_2368	0.34	-1.0
2,702,108	-		PP_2368	0.34	-0.7
2,702,108	-		PP_2368	0.34	-0.9
2,702,108	-		PP_2368	0.34	+0.1
2,702,167	+		PP_2368	0.41	-2.0
2,702,167	+		PP_2368	0.41	+0.2
2,702,311	+		PP_2368	0.59	-0.3
2,702,325	+		PP_2368	0.61	-0.9
2,702,431	+		PP_2368	0.74	-1.6
2,702,432	-		PP_2368	0.74	+0.4
2,702,432	-		PP_2368	0.74	-0.6
2,702,524	+		PP_2368	0.86	+0.9
2,702,525	-		PP_2368	0.86	-2.2
2,702,705	+				-0.4
2,702,706	-				-0.8
2,702,749	-				-2.5
2,702,776	+				+0.3
2,702,777	-				+2.2
2,702,781	-				-0.5
2,702,781	-				-2.4
2,702,860	+				-1.6
2,702,861	-				+0.1
2,702,986	+				+1.0
2,702,986	+				+0.8
2,702,987	-				+0.5
2,702,987	-				+0.4
2,702,987	-				+0.5
2,703,007	+				-2.2
2,703,076	+				+0.6
2,703,077	-				-0.5
2,703,143	-				-0.1
2,703,143	-				-2.6
2,703,146	+				+0.9
2,703,147	-				-0.1
2,703,200	+				-1.0
2,703,283	+		PP_2369	0.10	-0.8
2,703,284	-		PP_2369	0.11	+0.4
2,703,324	-		PP_2369	0.11	+0.3
2,703,646	-		PP_2369	0.19	-0.8
2,703,684	+		PP_2369	0.20	-0.5
2,703,684	+		PP_2369	0.20	+0.3
2,703,685	-		PP_2369	0.20	-1.0
2,703,685	-		PP_2369	0.20	-2.0
2,703,710	+		PP_2369	0.21	+0.2
2,703,730	-		PP_2369	0.21	-1.6
2,703,803	+		PP_2369	0.23	-0.3
2,703,804	-		PP_2369	0.23	-1.3
2,703,806	+		PP_2369	0.23	+0.9
2,703,806	+		PP_2369	0.23	-2.7
2,703,806	+		PP_2369	0.23	-1.4
2,703,806	+		PP_2369	0.23	+0.3
2,703,806	+		PP_2369	0.23	-1.2
2,703,807	-		PP_2369	0.23	+0.9
2,703,807	-		PP_2369	0.23	+0.5
2,703,807	-		PP_2369	0.23	+0.5
2,703,972	+		PP_2369	0.27	-0.3
2,704,049	+		PP_2369	0.29	-0.6
2,704,049	+		PP_2369	0.29	-0.9
2,704,078	-		PP_2369	0.29	+0.0
2,704,240	+		PP_2369	0.33	-1.6
2,704,241	-		PP_2369	0.33	+1.0
2,704,318	+		PP_2369	0.35	-1.5
2,704,374	+		PP_2369	0.37	+0.0
2,704,374	+		PP_2369	0.37	+0.8
2,704,409	+		PP_2369	0.37	-0.5
2,704,409	+		PP_2369	0.37	+0.0
2,704,437	+		PP_2369	0.38	+0.0
2,704,437	+		PP_2369	0.38	-1.7
2,704,518	+		PP_2369	0.40	-0.0
2,704,518	+		PP_2369	0.40	+0.6
2,704,519	-		PP_2369	0.40	+1.4
2,704,519	-		PP_2369	0.40	-1.7
2,704,570	+		PP_2369	0.41	+0.5
2,704,577	-		PP_2369	0.41	-1.4
2,705,115	-		PP_2369	0.54	+0.5
2,705,115	-		PP_2369	0.54	-1.9
2,705,117	+		PP_2369	0.54	-1.7
2,705,326	-		PP_2369	0.59	-2.4
2,705,326	-		PP_2369	0.59	+1.8
2,705,519	+		PP_2369	0.64	-2.1
2,705,565	+		PP_2369	0.65	-0.9
2,705,661	-		PP_2369	0.67	-0.5
2,705,661	-		PP_2369	0.67	-1.1
2,705,710	+		PP_2369	0.68	-1.1
2,705,710	+		PP_2369	0.68	+1.2
2,705,759	+		PP_2369	0.70	+0.2
2,706,167	-		PP_2369	0.79	+0.9
2,706,311	-				+1.2
2,706,328	-		PP_2369	0.83	+0.2
2,706,328	-		PP_2369	0.83	-0.2
2,706,368	+		PP_2369	0.84	+0.4
2,706,368	+		PP_2369	0.84	-1.3
2,706,400	+				+2.2
2,706,400	+		PP_2369	0.85	+0.0
2,706,524	-		PP_2369	0.88	-1.0
2,706,613	-				+0.2
2,706,668	+				-0.8
2,706,668	+				-0.8
2,706,668	+				-0.2
2,706,669	-				-1.0
2,706,825	+				+0.4
2,706,825	+				+1.1
2,706,826	-				+0.1
2,706,930	+				+0.9
2,706,931	-				+0.8
2,706,950	+				-1.3
2,707,125	-				-0.5
2,707,125	-				+0.9
2,707,304	+		PP_2370	0.46	-0.1
2,707,405	+		PP_2370	0.66	+1.6
2,707,405	+		PP_2370	0.66	+2.0
2,707,406	-		PP_2370	0.66	+0.2
2,707,406	-		PP_2370	0.66	-1.2
2,707,476	-		PP_2370	0.80	+0.6
2,707,557	+				+2.3
2,707,572	+				-0.7
2,707,572	+				+0.3
2,707,573	-				+0.5
2,707,604	-				+2.6
2,707,611	+				-0.3
2,707,611	+				+2.0
2,707,611	+				+0.4
2,707,612	-				+1.1
2,707,640	-				-0.4
2,707,768	-		PP_2371	0.13	+0.3
2,707,768	-		PP_2371	0.13	-1.5
2,707,884	+		PP_2371	0.20	-0.3
2,707,885	-		PP_2371	0.20	+1.8
2,707,885	-		PP_2371	0.20	+1.0
2,707,885	-		PP_2371	0.20	-0.2