Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2336 and PP_2337 are separated by 65 nucleotides PP_2337 and PP_2338 are separated by 166 nucleotides
PP_2336: PP_2336 - aconitate hydratase 1, at 2,664,138 to 2,666,726
_2336
PP_2337: PP_2337 - aconitate isomerase, at 2,666,792 to 2,667,982
_2337
PP_2338: PP_2338 - 2-methylcitrate dehydratase, at 2,668,149 to 2,669,633
_2338
Position (kb)
2666
2667
2668 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2665.932 kb on + strand, within PP_2336 at 2665.932 kb on + strand, within PP_2336 at 2665.956 kb on + strand, within PP_2336 at 2665.956 kb on + strand, within PP_2336 at 2665.956 kb on + strand, within PP_2336 at 2665.957 kb on - strand, within PP_2336 at 2665.957 kb on - strand, within PP_2336 at 2665.957 kb on - strand, within PP_2336 at 2665.957 kb on - strand, within PP_2336 at 2666.316 kb on + strand at 2666.316 kb on + strand, within PP_2336 at 2666.316 kb on + strand, within PP_2336 at 2666.316 kb on + strand, within PP_2336 at 2666.448 kb on - strand, within PP_2336 at 2666.545 kb on - strand at 2666.565 kb on - strand at 2666.623 kb on + strand at 2666.667 kb on + strand at 2666.667 kb on + strand at 2666.760 kb on + strand at 2666.761 kb on - strand at 2666.773 kb on + strand at 2666.803 kb on - strand at 2666.803 kb on - strand at 2666.828 kb on + strand at 2666.829 kb on - strand at 2666.829 kb on - strand at 2666.829 kb on - strand at 2667.196 kb on + strand, within PP_2337 at 2667.196 kb on + strand, within PP_2337 at 2667.416 kb on - strand, within PP_2337 at 2667.482 kb on + strand, within PP_2337 at 2667.483 kb on - strand, within PP_2337 at 2667.580 kb on + strand, within PP_2337 at 2667.653 kb on + strand, within PP_2337 at 2667.653 kb on + strand, within PP_2337 at 2668.053 kb on + strand at 2668.054 kb on - strand at 2668.079 kb on - strand at 2668.162 kb on + strand at 2668.163 kb on - strand at 2668.163 kb on - strand at 2668.163 kb on - strand at 2668.198 kb on + strand at 2668.199 kb on - strand at 2668.199 kb on - strand at 2668.216 kb on - strand at 2668.392 kb on + strand, within PP_2338 at 2668.452 kb on + strand, within PP_2338 at 2668.779 kb on - strand, within PP_2338 at 2668.779 kb on - strand, within PP_2338 at 2668.923 kb on + strand at 2668.923 kb on + strand, within PP_2338 at 2668.923 kb on + strand, within PP_2338 at 2668.923 kb on + strand, within PP_2338 at 2668.923 kb on + strand, within PP_2338 at 2668.924 kb on - strand, within PP_2338 at 2668.924 kb on - strand, within PP_2338 at 2668.944 kb on - strand, within PP_2338
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,665,932 + PP_2336 0.69 -0.1 2,665,932 + PP_2336 0.69 +0.8 2,665,956 + PP_2336 0.70 +1.1 2,665,956 + PP_2336 0.70 +1.3 2,665,956 + PP_2336 0.70 +0.1 2,665,957 - PP_2336 0.70 +0.9 2,665,957 - PP_2336 0.70 +0.3 2,665,957 - PP_2336 0.70 +2.8 2,665,957 - PP_2336 0.70 +1.4 2,666,316 + +0.7 2,666,316 + PP_2336 0.84 +1.0 2,666,316 + PP_2336 0.84 +1.6 2,666,316 + PP_2336 0.84 +0.4 2,666,448 - PP_2336 0.89 +0.8 2,666,545 - +0.6 2,666,565 - -0.0 2,666,623 + +1.5 2,666,667 + -0.4 2,666,667 + +1.8 2,666,760 + -1.3 2,666,761 - +0.1 2,666,773 + -0.5 2,666,803 - -1.0 2,666,803 - +1.7 2,666,828 + +1.0 2,666,829 - +0.8 2,666,829 - +0.3 2,666,829 - +1.0 2,667,196 + PP_2337 0.34 -0.5 2,667,196 + PP_2337 0.34 +1.7 2,667,416 - PP_2337 0.52 -0.5 2,667,482 + PP_2337 0.58 -1.0 2,667,483 - PP_2337 0.58 +0.8 2,667,580 + PP_2337 0.66 -1.3 2,667,653 + PP_2337 0.72 +0.4 2,667,653 + PP_2337 0.72 +0.3 2,668,053 + -2.1 2,668,054 - -1.1 2,668,079 - +0.2 2,668,162 + +0.3 2,668,163 - +2.5 2,668,163 - +0.5 2,668,163 - -0.4 2,668,198 + +0.8 2,668,199 - -0.8 2,668,199 - -0.3 2,668,216 - -1.0 2,668,392 + PP_2338 0.16 -1.1 2,668,452 + PP_2338 0.20 +0.7 2,668,779 - PP_2338 0.42 -1.0 2,668,779 - PP_2338 0.42 -2.8 2,668,923 + +4.8 2,668,923 + PP_2338 0.52 -1.2 2,668,923 + PP_2338 0.52 +0.3 2,668,923 + PP_2338 0.52 +0.8 2,668,923 + PP_2338 0.52 +0.3 2,668,924 - PP_2338 0.52 +0.5 2,668,924 - PP_2338 0.52 -1.3 2,668,944 - PP_2338 0.54 +0.6
Or see this region's nucleotide sequence