Strain Fitness in Pseudomonas putida KT2440 around PP_2327

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,653,338	+		PP_2325	0.76	-1.2
2,653,339	-		PP_2325	0.76	-0.7
2,653,339	-		PP_2325	0.76	+0.5
2,653,409	+		PP_2325	0.84	-0.3
2,653,409	+		PP_2325	0.84	+0.2
2,653,409	+		PP_2325	0.84	-1.0
2,653,410	-		PP_2325	0.84	-1.9
2,653,454	+		PP_2325	0.89	-1.5
2,653,454	+		PP_2325	0.89	-2.6
2,653,518	-				-0.2
2,653,643	-				+2.1
2,653,643	-				+0.7
2,653,704	+				-1.5
2,653,704	+				-1.4
2,653,705	-				+0.1
2,653,785	+		PP_2326	0.20	-2.1
2,653,785	+		PP_2326	0.20	+0.1
2,653,786	-		PP_2326	0.20	-0.4
2,653,786	-		PP_2326	0.20	-1.3
2,653,786	-		PP_2326	0.20	-0.5
2,653,786	-		PP_2326	0.20	+1.7
2,653,786	-		PP_2326	0.20	-1.6
2,653,786	-		PP_2326	0.20	+0.7
2,653,906	+		PP_2326	0.44	-0.1
2,653,906	+		PP_2326	0.44	-0.8
2,653,907	-		PP_2326	0.44	+0.4
2,653,907	-		PP_2326	0.44	+0.7
2,653,907	-		PP_2326	0.44	-1.1
2,653,907	-		PP_2326	0.44	+0.8
2,653,907	-		PP_2326	0.44	-1.6
2,653,918	+		PP_2326	0.47	+0.9
2,654,050	+		PP_2326	0.73	-1.8
2,654,051	-		PP_2326	0.73	+0.4
2,654,087	-		PP_2326	0.81	-0.0
2,654,087	-		PP_2326	0.81	-0.1
2,654,087	-		PP_2326	0.81	-1.1
2,654,139	+				-1.3
2,654,139	+				-0.2
2,654,139	+				-0.8
2,654,140	-				+1.0
2,654,140	-				+1.4
2,654,171	+				+0.5
2,654,187	+				+0.6
2,654,222	+				+0.7
2,654,255	-				+0.2
2,654,258	+				-0.2
2,654,259	-				+1.3
2,654,260	+				-1.2
2,654,260	+				+0.0
2,654,260	+				+0.2
2,654,262	+				-1.1
2,654,311	+				-0.1
2,654,311	+				-0.1
2,654,312	-				+2.3
2,654,312	-				-0.5
2,654,312	-				+1.0
2,654,333	+				+0.1
2,654,333	+				-0.6
2,654,334	-				+0.7
2,654,390	+				+0.3
2,654,390	+				+0.7
2,654,391	-				-1.0
2,654,391	-				-0.1
2,654,392	+				-0.9
2,654,392	+				-0.4
2,654,393	-				-0.7
2,654,393	-				+0.6
2,654,801	+		PP_2327	0.50	-1.1
2,654,801	+		PP_2327	0.50	-0.3
2,654,801	+		PP_2327	0.50	-0.5
2,654,801	+		PP_2327	0.50	+0.7
2,654,801	+		PP_2327	0.50	+1.9
2,654,802	-		PP_2327	0.50	+3.4
2,654,900	+		PP_2327	0.60	+0.7
2,654,900	+		PP_2327	0.60	-0.4
2,654,900	+		PP_2327	0.60	+1.6
2,654,901	-		PP_2327	0.60	+0.7
2,654,901	-		PP_2327	0.60	+1.2
2,655,008	+		PP_2327	0.71	+0.3
2,655,008	+		PP_2327	0.71	+0.1
2,655,008	+		PP_2327	0.71	+0.8
2,655,008	+		PP_2327	0.71	+0.3
2,655,008	+		PP_2327	0.71	+1.5
2,655,009	-		PP_2327	0.71	+2.5
2,655,009	-		PP_2327	0.71	+0.4
2,655,315	+				-0.9
2,655,436	+				-0.7
2,655,436	+				+0.6
2,655,452	+				+0.4
2,655,453	-				-0.0
2,655,639	-		PP_2328	0.25	-0.6
2,655,639	-		PP_2328	0.25	-0.0
2,655,639	-		PP_2328	0.25	+1.7
2,655,639	-		PP_2328	0.25	-0.4
2,655,640	+		PP_2328	0.25	+0.5
2,655,640	+		PP_2328	0.25	+0.2
2,655,641	-		PP_2328	0.25	+0.9
2,655,641	-		PP_2328	0.25	+1.3
2,655,641	-		PP_2328	0.25	+0.2
2,655,641	-		PP_2328	0.25	-0.4
2,655,641	-		PP_2328	0.25	+0.9
2,655,641	-		PP_2328	0.25	+1.0
2,655,641	-		PP_2328	0.25	+1.7
2,655,648	+		PP_2328	0.26	+0.1
2,655,688	+		PP_2328	0.32	-1.1
2,655,688	+		PP_2328	0.32	-0.1
2,655,688	+		PP_2328	0.32	+0.5
2,655,689	-		PP_2328	0.32	+2.3
2,656,014	+		PP_2328	0.76	-1.0
2,656,015	-		PP_2328	0.76	+1.0
2,656,021	-		PP_2328	0.77	+0.8
2,656,021	-		PP_2328	0.77	+2.5
2,656,150	+				+0.6
2,656,150	+				+0.0
2,656,151	-				-0.1
2,656,151	-				+1.9
2,656,229	+				+0.3
2,656,230	-				-0.4
2,656,232	+				-1.3
2,656,233	-				-1.5
2,656,233	-				-1.0
2,656,244	-				+0.8
2,656,262	+				+0.6