Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2308 and PP_2309 are separated by 41 nucleotides PP_2309 and PP_2310 are separated by 75 nucleotides PP_2310 and PP_2311 are separated by 126 nucleotides
PP_2308: PP_2308 - putative Acyl-CoA thioesterase II, at 2,637,895 to 2,638,692
_2308
PP_2309: PP_2309 - conserved protein of unknown function, at 2,638,734 to 2,639,102
_2309
PP_2310: PP_2310 - Methyl-accepting chemotaxis transducer, at 2,639,178 to 2,640,656
_2310
PP_2311: PP_2311 - Mg-dependent cytoplasmic DNase / RNase, at 2,640,783 to 2,641,580
_2311
Position (kb)
2639
2640
2641 Strain fitness (log2 ratio)
-2
-1
0
1 at 2638.292 kb on - strand, within PP_2308 at 2638.293 kb on + strand, within PP_2308 at 2638.293 kb on + strand, within PP_2308 at 2638.294 kb on - strand, within PP_2308 at 2638.509 kb on + strand, within PP_2308 at 2638.510 kb on - strand, within PP_2308 at 2638.510 kb on - strand, within PP_2308 at 2638.825 kb on - strand, within PP_2309 at 2639.179 kb on + strand at 2639.180 kb on - strand at 2639.181 kb on + strand at 2639.181 kb on + strand at 2639.181 kb on + strand at 2639.181 kb on + strand at 2639.181 kb on + strand at 2639.181 kb on + strand at 2639.181 kb on + strand at 2639.182 kb on - strand at 2639.182 kb on - strand at 2639.182 kb on - strand at 2639.326 kb on - strand, within PP_2310 at 2639.455 kb on + strand, within PP_2310 at 2639.456 kb on - strand, within PP_2310 at 2639.456 kb on - strand, within PP_2310 at 2639.599 kb on - strand, within PP_2310 at 2639.663 kb on + strand, within PP_2310 at 2639.664 kb on - strand, within PP_2310 at 2639.664 kb on - strand, within PP_2310 at 2639.664 kb on - strand, within PP_2310 at 2639.712 kb on + strand, within PP_2310 at 2639.788 kb on - strand, within PP_2310 at 2639.943 kb on + strand, within PP_2310 at 2640.029 kb on - strand, within PP_2310 at 2640.088 kb on - strand, within PP_2310 at 2640.232 kb on + strand, within PP_2310 at 2640.315 kb on + strand, within PP_2310 at 2640.316 kb on - strand, within PP_2310 at 2640.316 kb on - strand, within PP_2310 at 2640.316 kb on - strand, within PP_2310 at 2640.316 kb on - strand, within PP_2310 at 2640.416 kb on - strand, within PP_2310 at 2640.565 kb on + strand at 2640.565 kb on + strand at 2640.623 kb on - strand at 2640.623 kb on - strand at 2640.641 kb on + strand at 2640.641 kb on + strand at 2640.642 kb on - strand at 2640.743 kb on + strand at 2640.822 kb on - strand at 2640.947 kb on - strand, within PP_2311 at 2640.968 kb on + strand, within PP_2311 at 2640.968 kb on + strand, within PP_2311 at 2640.969 kb on - strand, within PP_2311 at 2641.073 kb on - strand, within PP_2311 at 2641.297 kb on - strand, within PP_2311 at 2641.587 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,638,292 - PP_2308 0.50 +0.3 2,638,293 + PP_2308 0.50 -1.6 2,638,293 + PP_2308 0.50 -0.7 2,638,294 - PP_2308 0.50 -0.0 2,638,509 + PP_2308 0.77 -1.2 2,638,510 - PP_2308 0.77 -2.0 2,638,510 - PP_2308 0.77 -0.4 2,638,825 - PP_2309 0.25 -0.9 2,639,179 + +0.0 2,639,180 - -1.6 2,639,181 + +0.8 2,639,181 + -1.3 2,639,181 + -1.3 2,639,181 + -0.4 2,639,181 + +1.1 2,639,181 + -1.5 2,639,181 + +0.3 2,639,182 - -0.9 2,639,182 - +0.6 2,639,182 - -0.3 2,639,326 - PP_2310 0.10 -0.1 2,639,455 + PP_2310 0.19 -0.2 2,639,456 - PP_2310 0.19 -0.2 2,639,456 - PP_2310 0.19 -2.3 2,639,599 - PP_2310 0.28 +0.1 2,639,663 + PP_2310 0.33 -1.7 2,639,664 - PP_2310 0.33 -2.5 2,639,664 - PP_2310 0.33 +0.8 2,639,664 - PP_2310 0.33 -0.4 2,639,712 + PP_2310 0.36 -1.0 2,639,788 - PP_2310 0.41 -1.9 2,639,943 + PP_2310 0.52 -1.7 2,640,029 - PP_2310 0.58 -1.2 2,640,088 - PP_2310 0.62 -0.7 2,640,232 + PP_2310 0.71 -1.1 2,640,315 + PP_2310 0.77 +0.2 2,640,316 - PP_2310 0.77 -0.8 2,640,316 - PP_2310 0.77 -0.0 2,640,316 - PP_2310 0.77 -0.1 2,640,316 - PP_2310 0.77 -0.0 2,640,416 - PP_2310 0.84 -0.2 2,640,565 + -0.0 2,640,565 + +1.0 2,640,623 - -0.0 2,640,623 - -0.6 2,640,641 + -1.9 2,640,641 + +0.0 2,640,642 - -0.2 2,640,743 + -1.3 2,640,822 - +0.6 2,640,947 - PP_2311 0.21 -0.1 2,640,968 + PP_2311 0.23 -1.2 2,640,968 + PP_2311 0.23 -0.3 2,640,969 - PP_2311 0.23 -0.2 2,641,073 - PP_2311 0.36 -1.2 2,641,297 - PP_2311 0.64 -0.5 2,641,587 - -2.2
Or see this region's nucleotide sequence