Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2306 and PP_2307 are separated by 14 nucleotides PP_2307 and PP_2308 are separated by 77 nucleotides PP_2308 and PP_2309 are separated by 41 nucleotides PP_2309 and PP_2310 are separated by 75 nucleotides
PP_2306: PP_2306 - conserved exported protein of unknown function, at 2,636,745 to 2,637,023
_2306
PP_2307: PP_2307 - conserved protein of unknown function, at 2,637,038 to 2,637,817
_2307
PP_2308: PP_2308 - putative Acyl-CoA thioesterase II, at 2,637,895 to 2,638,692
_2308
PP_2309: PP_2309 - conserved protein of unknown function, at 2,638,734 to 2,639,102
_2309
PP_2310: PP_2310 - Methyl-accepting chemotaxis transducer, at 2,639,178 to 2,640,656
_2310
Position (kb)
2637
2638
2639 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2636.899 kb on - strand, within PP_2306 at 2636.995 kb on - strand, within PP_2306 at 2637.037 kb on + strand at 2637.037 kb on + strand at 2637.037 kb on + strand at 2637.037 kb on + strand at 2637.037 kb on + strand at 2637.038 kb on - strand at 2637.038 kb on - strand at 2637.038 kb on - strand at 2637.038 kb on - strand at 2637.038 kb on - strand at 2637.038 kb on - strand at 2637.038 kb on - strand at 2637.072 kb on + strand at 2637.124 kb on + strand, within PP_2307 at 2637.251 kb on - strand, within PP_2307 at 2637.456 kb on + strand, within PP_2307 at 2637.822 kb on + strand at 2637.853 kb on + strand at 2637.854 kb on - strand at 2638.009 kb on + strand, within PP_2308 at 2638.009 kb on + strand, within PP_2308 at 2638.009 kb on + strand, within PP_2308 at 2638.010 kb on - strand, within PP_2308 at 2638.010 kb on - strand, within PP_2308 at 2638.010 kb on - strand, within PP_2308 at 2638.010 kb on - strand, within PP_2308 at 2638.292 kb on - strand, within PP_2308 at 2638.293 kb on + strand, within PP_2308 at 2638.293 kb on + strand, within PP_2308 at 2638.294 kb on - strand, within PP_2308 at 2638.509 kb on + strand, within PP_2308 at 2638.510 kb on - strand, within PP_2308 at 2638.510 kb on - strand, within PP_2308 at 2638.825 kb on - strand, within PP_2309 at 2639.179 kb on + strand at 2639.180 kb on - strand at 2639.181 kb on + strand at 2639.181 kb on + strand at 2639.181 kb on + strand at 2639.181 kb on + strand at 2639.181 kb on + strand at 2639.181 kb on + strand at 2639.181 kb on + strand at 2639.182 kb on - strand at 2639.182 kb on - strand at 2639.182 kb on - strand at 2639.326 kb on - strand, within PP_2310 at 2639.455 kb on + strand, within PP_2310 at 2639.456 kb on - strand, within PP_2310 at 2639.456 kb on - strand, within PP_2310 at 2639.599 kb on - strand, within PP_2310 at 2639.663 kb on + strand, within PP_2310 at 2639.664 kb on - strand, within PP_2310 at 2639.664 kb on - strand, within PP_2310 at 2639.664 kb on - strand, within PP_2310
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,636,899 - PP_2306 0.55 +1.0 2,636,995 - PP_2306 0.90 +0.0 2,637,037 + -1.3 2,637,037 + -0.9 2,637,037 + -0.8 2,637,037 + -0.2 2,637,037 + +2.2 2,637,038 - +0.1 2,637,038 - +0.5 2,637,038 - +0.7 2,637,038 - +1.3 2,637,038 - +2.0 2,637,038 - -1.1 2,637,038 - -1.0 2,637,072 + +1.2 2,637,124 + PP_2307 0.11 +0.8 2,637,251 - PP_2307 0.27 -0.1 2,637,456 + PP_2307 0.54 -1.2 2,637,822 + -1.4 2,637,853 + +1.0 2,637,854 - +0.9 2,638,009 + PP_2308 0.14 +0.6 2,638,009 + PP_2308 0.14 -0.3 2,638,009 + PP_2308 0.14 -1.4 2,638,010 - PP_2308 0.14 +0.9 2,638,010 - PP_2308 0.14 -0.5 2,638,010 - PP_2308 0.14 -0.9 2,638,010 - PP_2308 0.14 -1.9 2,638,292 - PP_2308 0.50 +0.3 2,638,293 + PP_2308 0.50 -1.6 2,638,293 + PP_2308 0.50 -0.7 2,638,294 - PP_2308 0.50 -0.0 2,638,509 + PP_2308 0.77 -1.2 2,638,510 - PP_2308 0.77 -2.0 2,638,510 - PP_2308 0.77 -0.4 2,638,825 - PP_2309 0.25 -0.9 2,639,179 + +0.0 2,639,180 - -1.6 2,639,181 + +0.8 2,639,181 + -1.3 2,639,181 + -1.3 2,639,181 + -0.4 2,639,181 + +1.1 2,639,181 + -1.5 2,639,181 + +0.3 2,639,182 - -0.9 2,639,182 - +0.6 2,639,182 - -0.3 2,639,326 - PP_2310 0.10 -0.1 2,639,455 + PP_2310 0.19 -0.2 2,639,456 - PP_2310 0.19 -0.2 2,639,456 - PP_2310 0.19 -2.3 2,639,599 - PP_2310 0.28 +0.1 2,639,663 + PP_2310 0.33 -1.7 2,639,664 - PP_2310 0.33 -2.5 2,639,664 - PP_2310 0.33 +0.8 2,639,664 - PP_2310 0.33 -0.4
Or see this region's nucleotide sequence