Strain Fitness in Pseudomonas putida KT2440 around PP_2241

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,555,154	+		PP_2240	0.60	-1.3
2,555,155	-		PP_2240	0.60	-2.3
2,555,155	-		PP_2240	0.60	+1.0
2,555,385	-		PP_2240	0.73	+1.4
2,555,385	-		PP_2240	0.73	-0.1
2,555,487	+		PP_2240	0.78	+0.7
2,555,524	-		PP_2240	0.80	-1.7
2,555,754	+				-0.5
2,555,754	+				-0.9
2,555,754	+				+0.4
2,555,755	-				+0.3
2,555,898	+				+2.7
2,555,899	-				-0.6
2,555,939	+				-2.0
2,555,940	-				-2.2
2,555,940	-				+0.0
2,555,977	+				+0.2
2,555,978	-				-0.5
2,556,051	-				+0.1
2,556,076	+				-0.9
2,556,211	-				-1.4
2,556,424	-		PP_2241	0.26	-0.6
2,556,481	+		PP_2241	0.31	+0.1
2,556,496	+		PP_2241	0.32	+1.8
2,556,497	-		PP_2241	0.32	+2.1
2,556,514	-		PP_2241	0.34	-1.1
2,556,514	-		PP_2241	0.34	-1.9
2,556,514	-		PP_2241	0.34	+1.7
2,556,549	+		PP_2241	0.36	-1.1
2,556,549	+		PP_2241	0.36	+2.1
2,556,550	-		PP_2241	0.37	-2.3
2,556,550	-		PP_2241	0.37	+0.6
2,556,632	-		PP_2241	0.43	+1.8
2,556,632	-		PP_2241	0.43	+0.0
2,556,732	-		PP_2241	0.52	+1.4
2,556,733	+		PP_2241	0.52	-0.6
2,556,734	-		PP_2241	0.52	+0.9
2,556,734	-		PP_2241	0.52	-1.6
2,556,734	-		PP_2241	0.52	-0.6
2,556,734	-		PP_2241	0.52	-2.0
2,556,734	-		PP_2241	0.52	+0.6
2,556,734	-		PP_2241	0.52	+0.7
2,556,734	-		PP_2241	0.52	-0.2
2,556,762	+		PP_2241	0.54	-0.2
2,556,762	+		PP_2241	0.54	-0.1
2,556,807	+		PP_2241	0.58	+0.6
2,556,808	-		PP_2241	0.58	+1.3
2,556,840	+		PP_2241	0.61	-0.9
2,556,841	-		PP_2241	0.61	-0.3
2,557,003	-		PP_2241	0.74	+0.6
2,557,003	-		PP_2241	0.74	+0.5
2,557,059	-		PP_2241	0.79	-0.1
2,557,174	+		PP_2241	0.89	+1.2
2,557,174	+		PP_2241	0.89	+0.5
2,557,174	+				+3.8
2,557,174	+		PP_2241	0.89	-0.8
2,557,175	-		PP_2241	0.89	-1.1
2,557,175	-		PP_2241	0.89	+0.5
2,557,175	-		PP_2241	0.89	-1.4
2,557,175	-		PP_2241	0.89	+1.1
2,557,175	-		PP_2241	0.89	-1.8
2,557,331	+				+0.8
2,557,344	+				+0.3
2,557,344	+				+1.8
2,557,345	-				-0.6
2,557,345	-				+0.6
2,557,382	+				+2.2
2,557,383	-				-1.3
2,557,419	+				+1.1
2,557,420	-				+0.9
2,557,435	+				+0.9
2,557,436	-				+1.1
2,557,483	+				-0.9
2,557,503	+				-0.3
2,557,590	-				-0.3
2,557,632	-				-0.3
2,557,736	+				-1.3
2,557,736	+				+1.3
2,557,737	-				-0.5
2,558,145	-		PP_2242	0.18	-0.6
2,558,149	+		PP_2242	0.18	+0.5
2,558,173	-		PP_2242	0.19	+1.4