Strain Fitness in Pseudomonas putida KT2440 around PP_2235

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,541,691	-		PP_2234	0.23	+0.8
2,541,796	+		PP_2234	0.33	-0.6
2,541,796	+		PP_2234	0.33	+0.8
2,541,796	+		PP_2234	0.33	-0.6
2,541,797	-		PP_2234	0.33	+3.8
2,541,884	+		PP_2234	0.42	-1.2
2,541,884	+		PP_2234	0.42	+1.2
2,541,884	+		PP_2234	0.42	-0.8
2,541,884	+		PP_2234	0.42	-2.0
2,541,925	-		PP_2234	0.46	+0.8
2,542,055	+		PP_2234	0.59	-0.3
2,542,055	+		PP_2234	0.59	-2.0
2,542,271	+		PP_2234	0.81	+1.1
2,542,271	+		PP_2234	0.81	-1.0
2,542,409	-				-0.7
2,542,409	-				+1.6
2,542,458	-				-1.7
2,542,520	+				+0.9
2,542,521	-				+0.6
2,542,521	-				-0.2
2,542,521	-				+0.3
2,542,521	-				-1.3
2,542,524	+				+0.6
2,542,525	-				-0.8
2,542,525	-				-1.3
2,542,525	-				-2.0
2,542,593	+		PP_2235	0.14	+1.3
2,542,593	+		PP_2235	0.14	-0.1
2,542,594	-		PP_2235	0.14	-0.3
2,542,594	-		PP_2235	0.14	-0.6
2,542,600	+		PP_2235	0.15	-1.0
2,542,600	+		PP_2235	0.15	-1.5
2,542,600	+		PP_2235	0.15	+0.9
2,542,600	+		PP_2235	0.15	+0.7
2,542,600	+		PP_2235	0.15	-0.4
2,542,601	-		PP_2235	0.16	-1.0
2,542,601	-		PP_2235	0.16	+1.3
2,542,604	+		PP_2235	0.17	-0.7
2,542,604	+		PP_2235	0.17	-0.2
2,542,605	-		PP_2235	0.17	+1.5
2,542,605	-		PP_2235	0.17	-1.1
2,542,722	-		PP_2235	0.48	-1.2
2,542,907	+				-1.1
2,542,908	-				-1.5
2,542,919	+				-1.7
2,542,920	-				-0.2
2,542,986	+		PP_2236	0.11	-0.1
2,542,986	+		PP_2236	0.11	-2.5
2,542,986	+		PP_2236	0.11	-0.5
2,542,986	+		PP_2236	0.11	-0.4
2,542,987	-		PP_2236	0.11	+0.1
2,542,988	+		PP_2236	0.11	-0.2
2,542,989	-		PP_2236	0.12	-0.2
2,543,082	-		PP_2236	0.24	-2.4
2,543,082	-		PP_2236	0.24	+0.1
2,543,094	+		PP_2236	0.25	-0.5
2,543,095	-		PP_2236	0.25	+0.6
2,543,108	+		PP_2236	0.27	+0.9
2,543,108	+		PP_2236	0.27	+0.5
2,543,108	+		PP_2236	0.27	-0.2
2,543,108	+		PP_2236	0.27	-0.4
2,543,108	+		PP_2236	0.27	-1.8
2,543,108	+		PP_2236	0.27	-0.1
2,543,108	+		PP_2236	0.27	-1.9
2,543,109	-		PP_2236	0.27	-1.5
2,543,109	-		PP_2236	0.27	+0.5
2,543,137	+		PP_2236	0.31	-1.5
2,543,137	+		PP_2236	0.31	+0.5
2,543,137	+		PP_2236	0.31	-1.1
2,543,137	+		PP_2236	0.31	-0.3
2,543,137	+		PP_2236	0.31	+1.2
2,543,137	+				+0.6
2,543,137	+		PP_2236	0.31	-0.9
2,543,137	+		PP_2236	0.31	-0.5
2,543,137	+		PP_2236	0.31	-0.7
2,543,137	+		PP_2236	0.31	+0.4
2,543,138	-		PP_2236	0.31	-1.1
2,543,138	-		PP_2236	0.31	-0.7
2,543,138	-		PP_2236	0.31	+0.8
2,543,138	-		PP_2236	0.31	+0.8
2,543,138	-		PP_2236	0.31	-0.0
2,543,138	-		PP_2236	0.31	+1.3
2,543,138	-		PP_2236	0.31	-0.7
2,543,138	-		PP_2236	0.31	-0.3
2,543,138	-		PP_2236	0.31	+0.5
2,543,138	-		PP_2236	0.31	-0.4
2,543,138	-		PP_2236	0.31	+1.9
2,543,138	-		PP_2236	0.31	-1.7
2,543,267	+		PP_2236	0.48	-1.3
2,543,267	+		PP_2236	0.48	-0.5
2,543,267	+		PP_2236	0.48	-0.5
2,543,268	-		PP_2236	0.48	+0.3
2,543,268	-		PP_2236	0.48	-2.3
2,543,342	+		PP_2236	0.57	-0.6
2,543,342	+		PP_2236	0.57	-1.0
2,543,342	+		PP_2236	0.57	+0.2
2,543,405	+		PP_2236	0.65	-0.5
2,543,405	+		PP_2236	0.65	-1.0
2,543,405	+		PP_2236	0.65	+0.7
2,543,406	-		PP_2236	0.66	+0.6
2,543,520	+		PP_2236	0.80	-1.0
2,543,769	-		PP_2237	0.10	+1.6