Strain Fitness in Pseudomonas putida KT2440 around PP_2228

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,533,231	+		PP_2225	0.68	-0.1
2,533,254	+		PP_2225	0.75	-1.0
2,533,255	-		PP_2225	0.75	-0.2
2,533,255	-		PP_2225	0.75	-0.1
2,533,255	-		PP_2225	0.75	-0.2
2,533,255	-		PP_2225	0.75	-1.7
2,533,256	+		PP_2225	0.75	-1.3
2,533,256	+		PP_2225	0.75	+1.1
2,533,257	-		PP_2225	0.75	-1.1
2,533,329	+				-1.5
2,533,434	+		PP_2226	0.33	-0.1
2,533,434	+		PP_2226	0.33	-1.4
2,533,491	+		PP_2226	0.53	-0.2
2,533,491	+		PP_2226	0.53	+0.8
2,533,492	-		PP_2226	0.53	-1.7
2,533,492	-		PP_2226	0.53	-0.1
2,533,492	-		PP_2226	0.53	+0.7
2,533,492	-		PP_2226	0.53	-0.5
2,533,492	-		PP_2226	0.53	-0.8
2,533,498	-		PP_2226	0.56	+0.4
2,533,498	-		PP_2226	0.56	+2.4
2,533,649	+				+0.3
2,533,649	+				-0.8
2,533,649	+				-1.1
2,533,649	+				-0.3
2,533,650	-				-1.8
2,533,650	-				-0.4
2,533,650	-				+0.5
2,533,650	-				+0.5
2,533,650	-				-0.1
2,533,669	+		PP_2227	0.10	+1.4
2,533,670	-		PP_2227	0.11	+1.0
2,533,670	-		PP_2227	0.11	-1.0
2,533,670	-		PP_2227	0.11	-1.2
2,533,670	-		PP_2227	0.11	-0.9
2,533,816	+		PP_2227	0.40	-0.1
2,533,816	+		PP_2227	0.40	-0.4
2,533,817	-		PP_2227	0.41	-1.4
2,533,817	-		PP_2227	0.41	+0.2
2,533,817	-		PP_2227	0.41	-0.3
2,533,817	-		PP_2227	0.41	-0.6
2,533,817	-		PP_2227	0.41	-0.2
2,533,817	-		PP_2227	0.41	-0.2
2,533,945	+		PP_2227	0.67	-0.0
2,534,119	-				+0.7
2,534,119	-				-0.1
2,534,171	+				+0.9
2,534,172	-				-0.5
2,534,172	-				+0.8
2,534,172	-				+1.0
2,534,173	+				+2.6
2,534,173	+				+2.3
2,534,173	+				+1.2
2,534,173	+				-1.2
2,534,173	+				-0.9
2,534,174	-				+0.3
2,534,174	-				-1.0
2,534,174	-				-0.5
2,534,174	-				+1.4
2,534,174	-				+0.8
2,534,174	-				+1.4
2,534,215	+				-1.0
2,534,290	+		PP_2228	0.11	+0.4
2,534,291	-		PP_2228	0.11	+0.5
2,534,526	+		PP_2228	0.25	+0.7
2,534,589	+		PP_2228	0.29	-0.8
2,534,589	+		PP_2228	0.29	+0.5
2,534,589	+		PP_2228	0.29	-1.8
2,534,590	-		PP_2228	0.29	-0.4
2,534,764	+		PP_2228	0.39	-0.3
2,534,764	+		PP_2228	0.39	-1.5
2,534,840	+		PP_2228	0.44	-0.0
2,534,841	-		PP_2228	0.44	+0.9
2,534,855	+		PP_2228	0.45	-0.2
2,534,855	+		PP_2228	0.45	-1.3
2,534,856	-		PP_2228	0.45	+0.8
2,534,856	-		PP_2228	0.45	+0.1
2,534,856	-		PP_2228	0.45	+0.5
2,535,001	+		PP_2228	0.53	-0.3
2,535,001	+		PP_2228	0.53	-1.1
2,535,002	-		PP_2228	0.54	+0.5
2,535,062	-		PP_2228	0.57	-0.2
2,535,096	-		PP_2228	0.59	+0.3
2,535,169	-		PP_2228	0.63	-0.2
2,535,224	-		PP_2228	0.67	-0.3
2,535,280	+		PP_2228	0.70	+0.1
2,535,280	+		PP_2228	0.70	-0.2
2,535,281	-		PP_2228	0.70	+0.3
2,535,281	-		PP_2228	0.70	+0.5
2,535,335	-		PP_2228	0.73	-2.3
2,535,335	-				+1.0
2,535,335	-		PP_2228	0.73	-2.1
2,535,404	-		PP_2228	0.77	-1.2
2,535,404	-		PP_2228	0.77	-1.9
2,535,478	-		PP_2228	0.82	-0.7
2,535,731	+				-1.4
2,535,922	-		PP_2229	0.41	+0.7
2,535,922	-		PP_2229	0.41	-0.5
2,536,015	-		PP_2229	0.68	-1.0
2,536,015	-		PP_2229	0.68	+0.3
2,536,047	+		PP_2229	0.78	-1.7
2,536,047	+		PP_2229	0.78	+0.3
2,536,047	-		PP_2229	0.78	+1.3
2,536,048	-		PP_2229	0.78	-0.6
2,536,048	-		PP_2229	0.78	+0.4
2,536,169	+				-0.0
2,536,235	+				+0.9
2,536,235	+				+0.4
2,536,235	+				-0.1
2,536,235	+				-0.1
2,536,235	+				-1.0
2,536,235	+				+0.4
2,536,236	-				+0.6
2,536,238	-				+2.0
2,536,445	+		PP_2230	0.11	-1.0
2,536,446	-		PP_2230	0.11	+0.2
2,536,527	-		PP_2230	0.14	-0.8
2,536,527	-		PP_2230	0.14	+0.2
2,536,603	-		PP_2230	0.16	+1.3
2,536,603	-		PP_2230	0.16	-0.4
2,536,745	-		PP_2230	0.21	-1.7
2,536,745	-		PP_2230	0.21	+0.2