Strain Fitness in Pseudomonas putida KT2440 around PP_2208

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,513,712	+		PP_2207	0.41	+0.8
2,513,783	+		PP_2207	0.48	-1.1
2,513,783	+		PP_2207	0.48	+0.2
2,513,784	-		PP_2207	0.48	+0.3
2,514,197	+		PP_2207	0.86	-1.6
2,514,197	+		PP_2207	0.86	-1.1
2,514,198	-		PP_2207	0.87	+0.2
2,514,198	-		PP_2207	0.87	-1.0
2,514,338	+				-1.7
2,514,338	+				-0.8
2,514,338	+				-0.5
2,514,339	-				-0.2
2,514,339	-				+0.1
2,514,513	+				+0.1
2,514,513	+				-1.9
2,514,540	+				+0.8
2,514,540	+				+0.9
2,514,540	+				-0.2
2,514,540	+				+0.6
2,514,540	+				-0.5
2,514,541	-				-0.4
2,514,541	-				+0.5
2,514,541	-				-1.2
2,514,648	-		PP_2208	0.11	-1.0
2,514,844	+				+0.8
2,514,845	-		PP_2208	0.35	-1.6
2,514,928	+		PP_2208	0.45	-0.7
2,514,929	-		PP_2208	0.45	+0.3
2,514,929	-		PP_2208	0.45	+0.1
2,514,929	-		PP_2208	0.45	+0.2
2,514,944	+		PP_2208	0.46	-0.1
2,514,944	+		PP_2208	0.46	-0.5
2,514,945	-		PP_2208	0.47	-1.1
2,515,118	+		PP_2208	0.68	-1.0
2,515,119	-		PP_2208	0.68	+0.4
2,515,119	-		PP_2208	0.68	-1.4
2,515,209	+		PP_2208	0.79	-0.7
2,515,210	-		PP_2208	0.79	+1.0
2,515,379	+				+0.3
2,515,379	+				+0.7
2,515,380	-				-1.5
2,515,380	-				-2.6
2,515,380	-				-0.1
2,515,426	+				+1.0
2,515,585	-		PP_2209	0.15	+0.7
2,515,585	-		PP_2209	0.15	-0.1
2,515,762	-		PP_2209	0.31	-0.4
2,515,762	-		PP_2209	0.31	+0.1
2,515,762	-		PP_2209	0.31	+0.6
2,515,762	-		PP_2209	0.31	+0.3
2,515,839	+		PP_2209	0.38	+1.0
2,515,839	+		PP_2209	0.38	-0.7
2,515,839	+		PP_2209	0.38	+1.2
2,515,840	-		PP_2209	0.38	+0.4
2,516,214	+		PP_2209	0.72	-0.2
2,516,215	-		PP_2209	0.72	-1.3
2,516,215	-		PP_2209	0.72	-1.6