Strain Fitness in Pseudomonas putida KT2440 around PP_2100

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,395,092	+		PP_2098	0.79	+1.3
2,395,092	+		PP_2098	0.79	+0.1
2,395,093	-		PP_2098	0.79	-1.0
2,395,093	-		PP_2098	0.79	+1.0
2,395,097	+		PP_2098	0.79	+0.3
2,395,111	-		PP_2098	0.80	+1.9
2,395,142	+		PP_2098	0.82	-2.3
2,395,143	-		PP_2098	0.82	-0.1
2,395,143	-		PP_2098	0.82	+1.2
2,395,193	+		PP_2098	0.86	-0.5
2,395,194	-		PP_2098	0.86	-2.1
2,395,194	-		PP_2098	0.86	-1.1
2,395,408	+				-0.7
2,395,408	+				-0.3
2,395,409	-				+0.5
2,395,409	-				-2.6
2,395,479	+				+1.2
2,395,503	-				-1.1
2,395,516	+				-0.6
2,395,516	+				+0.1
2,395,516	+				-0.5
2,395,570	-				-0.2
2,395,633	+				-0.8
2,395,633	+				-0.7
2,395,633	+				+0.4
2,395,633	+				+0.1
2,395,633	+				+0.7
2,395,633	+				+0.3
2,395,634	-				-1.4
2,395,634	-				+1.2
2,395,634	-				-0.1
2,395,634	-				-0.9
2,395,634	-				+0.0
2,395,652	+				-0.9
2,395,807	-		PP_2099	0.50	-0.0
2,395,807	-		PP_2099	0.50	+0.9
2,395,810	+		PP_2099	0.51	-1.4
2,395,810	+		PP_2099	0.51	-0.6
2,395,810	+		PP_2099	0.51	-0.1
2,395,810	+		PP_2099	0.51	+0.8
2,395,810	+		PP_2099	0.51	+0.2
2,395,810	+		PP_2099	0.51	-0.8
2,395,810	+		PP_2099	0.51	+1.6
2,395,811	-		PP_2099	0.51	-0.2
2,395,811	-		PP_2099	0.51	+0.5
2,395,811	-		PP_2099	0.51	+0.7
2,395,868	+		PP_2099	0.68	+1.6
2,395,994	-				+0.5
2,395,994	-				+0.2
2,395,997	+				-0.3
2,396,000	+				+0.1
2,396,000	+				+0.5
2,396,015	+				+0.1
2,396,016	-				+1.7
2,396,022	+				+0.1
2,396,022	+				+0.3
2,396,023	-				+1.4
2,396,023	-				-0.1
2,396,023	-				-0.8
2,396,023	-				-1.5
2,396,098	+				+1.2
2,396,098	+				+0.9
2,396,377	-				-1.4
2,396,525	-		PP_2100	0.14	+0.4
2,396,525	-		PP_2100	0.14	-0.2
2,396,709	+		PP_2100	0.19	-0.4
2,397,371	+		PP_2100	0.37	-1.4
2,397,371	+		PP_2100	0.37	+2.6
2,397,372	-		PP_2100	0.37	+0.3
2,397,645	+		PP_2100	0.45	+0.0
2,397,663	+		PP_2100	0.45	+2.1
2,397,663	+		PP_2100	0.45	-0.4
2,397,664	-		PP_2100	0.45	-0.2
2,397,664	-		PP_2100	0.45	+0.1
2,397,664	-		PP_2100	0.45	-0.0
2,397,843	+		PP_2100	0.50	-1.1
2,397,843	+		PP_2100	0.50	+0.6
2,397,843	+		PP_2100	0.50	-0.1
2,397,844	-		PP_2100	0.50	-1.1
2,397,849	+		PP_2100	0.50	-0.2
2,397,849	+		PP_2100	0.50	-0.3
2,397,850	-		PP_2100	0.50	+0.5
2,397,850	-		PP_2100	0.50	+0.9
2,397,850	-		PP_2100	0.50	+1.4
2,397,888	+		PP_2100	0.51	+1.0
2,397,888	+		PP_2100	0.51	+0.9
2,397,888	+		PP_2100	0.51	-0.9
2,397,888	+		PP_2100	0.51	+0.5
2,397,888	+		PP_2100	0.51	+2.2
2,397,888	+		PP_2100	0.51	-1.2
2,397,888	+		PP_2100	0.51	-0.2
2,397,889	-		PP_2100	0.51	-1.1
2,397,889	-		PP_2100	0.51	-0.5
2,397,889	-		PP_2100	0.51	-2.0
2,397,889	-		PP_2100	0.51	+0.6
2,397,889	-		PP_2100	0.51	+1.6
2,397,889	-		PP_2100	0.51	-0.5
2,397,889	-		PP_2100	0.51	-1.5
2,398,062	+		PP_2100	0.56	+1.0
2,398,063	-		PP_2100	0.56	-1.3
2,398,365	-		PP_2100	0.64	+0.4
2,398,365	-		PP_2100	0.64	+0.7
2,398,407	+		PP_2100	0.66	-1.0
2,398,408	-		PP_2100	0.66	+0.6
2,398,414	+		PP_2100	0.66	-0.8
2,398,488	+		PP_2100	0.68	+1.1
2,398,488	+		PP_2100	0.68	-0.0
2,398,488	+		PP_2100	0.68	+0.2
2,398,489	-		PP_2100	0.68	-0.9
2,398,489	-		PP_2100	0.68	+0.9
2,398,503	+		PP_2100	0.68	+0.6
2,398,504	-		PP_2100	0.68	-2.4
2,398,707	+		PP_2100	0.74	-0.5
2,398,707	+		PP_2100	0.74	-1.7
2,398,708	-		PP_2100	0.74	-0.3
2,399,004	+		PP_2100	0.82	-0.7
2,399,005	-		PP_2100	0.82	-0.8
2,399,005	-		PP_2100	0.82	+0.8
2,399,005	-		PP_2100	0.82	+0.7
2,399,005	-		PP_2100	0.82	-0.3
2,399,020	+		PP_2100	0.82	+0.9
2,399,139	+		PP_2100	0.86	-0.4
2,399,139	+		PP_2100	0.86	+0.9
2,399,140	-		PP_2100	0.86	+0.4
2,399,140	-		PP_2100	0.86	-1.1
2,399,140	-		PP_2100	0.86	+2.5
2,399,145	+		PP_2100	0.86	+0.1
2,399,145	+		PP_2100	0.86	+1.3
2,399,145	+		PP_2100	0.86	+0.9
2,399,146	-		PP_2100	0.86	+0.9
2,399,216	+		PP_2100	0.88	+2.3
2,399,235	+		PP_2100	0.88	+0.4
2,399,275	-		PP_2100	0.89	-1.0
2,399,397	+				-1.2
2,399,398	-				+0.1
2,399,419	-				-0.4
2,399,561	+				+0.8
2,399,561	+				-1.2
2,399,567	-				+0.7
2,399,741	+		PP_2101	0.13	+0.9
2,399,741	+		PP_2101	0.13	-2.7
2,399,742	-		PP_2101	0.13	+0.4
2,399,909	+		PP_2101	0.40	+0.4
2,399,927	-		PP_2101	0.43	+2.0
2,399,927	-		PP_2101	0.43	-2.1
2,399,978	+		PP_2101	0.51	-2.4
2,400,008	+				+0.2
2,400,100	+		PP_2101	0.71	-0.3
2,400,201	-		PP_2101	0.87	+0.4
2,400,201	-		PP_2101	0.87	-0.9
2,400,261	+				-0.1
2,400,261	+				+0.3
2,400,265	+				-0.5
2,400,265	+				+0.9
2,400,265	+				+0.9
2,400,266	-				+1.0
2,400,266	-				+1.3
2,400,366	+				+0.1
2,400,366	+				-1.0
2,400,366	+				-0.1
2,400,366	+				-0.2
2,400,368	+				-1.6
2,400,389	+				+0.1
2,400,421	+				+0.1
2,400,421	+				+1.4
2,400,458	-				+1.8
2,400,478	-				-1.8
2,400,606	+				-0.2
2,400,606	+				-2.3
2,400,606	+				-0.7
2,400,606	+				+0.9
2,400,606	+				+0.8
2,400,606	+				+0.2
2,400,606	+				+0.7
2,400,607	-				-0.8
2,400,607	-				+0.2
2,400,607	-				+0.6
2,400,607	-				+0.1
2,400,607	-				-0.3
2,400,607	-				-2.4