Strain Fitness in Pseudomonas putida KT2440 around PP_2074

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,358,141	+		PP_2072	0.79	-1.1
2,358,141	+		PP_2072	0.79	-0.5
2,358,141	+		PP_2072	0.79	+1.6
2,358,141	+		PP_2072	0.79	-1.4
2,358,142	-		PP_2072	0.80	+0.7
2,358,170	-		PP_2072	0.83	+1.9
2,358,183	+		PP_2072	0.84	-0.6
2,358,184	-		PP_2072	0.85	-1.3
2,358,187	+		PP_2072	0.85	+0.7
2,358,188	-		PP_2072	0.85	+1.0
2,358,188	-				+2.8
2,358,262	+				-0.3
2,358,263	-				-1.6
2,358,418	+		PP_2073	0.17	+1.0
2,358,418	+		PP_2073	0.17	-2.1
2,358,609	+		PP_2073	0.54	-2.7
2,358,790	+		PP_2073	0.89	-0.8
2,358,791	-		PP_2073	0.89	-0.9
2,358,936	-				+0.0
2,358,957	-				-0.2
2,359,265	+		PP_2074	0.24	+0.8
2,359,266	-		PP_2074	0.24	-0.5
2,359,454	+		PP_2074	0.44	+0.6
2,359,501	+		PP_2074	0.50	+0.4
2,359,548	+		PP_2074	0.55	-0.4
2,359,549	-		PP_2074	0.55	+0.1
2,359,549	-		PP_2074	0.55	+1.6
2,359,570	-				+1.5
2,359,785	-		PP_2074	0.80	+2.2
2,359,931	+				+0.2
2,359,931	+				+1.7
2,359,931	+				+1.3
2,359,931	+				+0.0
2,359,931	+				+0.6
2,359,931	+				+1.4
2,359,932	-				+0.0
2,359,932	-				+0.7
2,359,944	+				-1.7
2,360,433	-		PP_2075	0.36	+1.1
2,360,538	-		PP_2075	0.45	-0.7
2,360,672	-		PP_2075	0.57	+0.1
2,360,726	-		PP_2075	0.62	-2.4
2,360,753	+		PP_2075	0.64	+1.4
2,360,754	-		PP_2075	0.64	+0.4
2,360,783	-		PP_2075	0.67	-0.5
2,360,784	+		PP_2075	0.67	+0.6
2,360,799	+		PP_2075	0.68	+0.4
2,360,863	+		PP_2075	0.74	-1.5
2,360,864	-		PP_2075	0.74	-1.3
2,360,910	+		PP_2075	0.78	+1.8
2,360,911	-		PP_2075	0.78	-2.2
2,360,913	+		PP_2075	0.78	+0.8