Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2071 and PP_2072 are separated by 108 nucleotides PP_2072 and PP_2073 are separated by 19 nucleotides PP_2073 and PP_2074 are separated by 197 nucleotides
PP_2071: PP_2071 - conserved protein of unknown function, at 2,356,991 to 2,357,377
_2071
PP_2072: PP_2072 - Transcriptional regulator, AraC family, at 2,357,486 to 2,358,310
_2072
PP_2073: PP_2073 - Acetyltransferase, GNAT family, at 2,358,330 to 2,358,845
_2073
PP_2074: PP_2074 - Transcriptional regulator, LysR family, at 2,359,043 to 2,359,966
_2074
Position (kb)
2358
2359 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2357.409 kb on + strand at 2357.409 kb on + strand at 2357.436 kb on + strand at 2357.437 kb on - strand at 2357.519 kb on + strand at 2357.536 kb on - strand at 2357.682 kb on + strand, within PP_2072 at 2357.683 kb on - strand, within PP_2072 at 2357.753 kb on + strand, within PP_2072 at 2357.753 kb on + strand, within PP_2072 at 2357.753 kb on + strand, within PP_2072 at 2357.754 kb on - strand, within PP_2072 at 2357.809 kb on - strand, within PP_2072 at 2357.809 kb on - strand, within PP_2072 at 2357.871 kb on + strand, within PP_2072 at 2357.871 kb on + strand, within PP_2072 at 2357.871 kb on + strand, within PP_2072 at 2357.872 kb on - strand, within PP_2072 at 2357.921 kb on + strand, within PP_2072 at 2357.944 kb on - strand, within PP_2072 at 2358.030 kb on + strand, within PP_2072 at 2358.141 kb on + strand, within PP_2072 at 2358.141 kb on + strand, within PP_2072 at 2358.141 kb on + strand, within PP_2072 at 2358.141 kb on + strand, within PP_2072 at 2358.142 kb on - strand, within PP_2072 at 2358.170 kb on - strand, within PP_2072 at 2358.183 kb on + strand, within PP_2072 at 2358.184 kb on - strand, within PP_2072 at 2358.187 kb on + strand, within PP_2072 at 2358.188 kb on - strand, within PP_2072 at 2358.188 kb on - strand at 2358.262 kb on + strand at 2358.263 kb on - strand at 2358.418 kb on + strand, within PP_2073 at 2358.418 kb on + strand, within PP_2073 at 2358.609 kb on + strand, within PP_2073 at 2358.790 kb on + strand, within PP_2073 at 2358.791 kb on - strand, within PP_2073 at 2358.936 kb on - strand at 2358.957 kb on - strand at 2359.265 kb on + strand, within PP_2074 at 2359.266 kb on - strand, within PP_2074 at 2359.454 kb on + strand, within PP_2074 at 2359.501 kb on + strand, within PP_2074 at 2359.548 kb on + strand, within PP_2074 at 2359.549 kb on - strand, within PP_2074 at 2359.549 kb on - strand, within PP_2074 at 2359.570 kb on - strand at 2359.785 kb on - strand, within PP_2074
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,357,409 + -1.9 2,357,409 + -0.2 2,357,436 + +0.9 2,357,437 - +0.5 2,357,519 + +0.1 2,357,536 - -1.9 2,357,682 + PP_2072 0.24 -2.4 2,357,683 - PP_2072 0.24 +1.8 2,357,753 + PP_2072 0.32 -0.4 2,357,753 + PP_2072 0.32 -0.6 2,357,753 + PP_2072 0.32 +0.1 2,357,754 - PP_2072 0.32 -0.2 2,357,809 - PP_2072 0.39 +0.1 2,357,809 - PP_2072 0.39 +0.6 2,357,871 + PP_2072 0.47 +0.4 2,357,871 + PP_2072 0.47 -1.2 2,357,871 + PP_2072 0.47 +2.0 2,357,872 - PP_2072 0.47 +0.8 2,357,921 + PP_2072 0.53 -0.8 2,357,944 - PP_2072 0.56 +0.7 2,358,030 + PP_2072 0.66 +0.0 2,358,141 + PP_2072 0.79 -1.1 2,358,141 + PP_2072 0.79 -0.5 2,358,141 + PP_2072 0.79 +1.6 2,358,141 + PP_2072 0.79 -1.4 2,358,142 - PP_2072 0.80 +0.7 2,358,170 - PP_2072 0.83 +1.9 2,358,183 + PP_2072 0.84 -0.6 2,358,184 - PP_2072 0.85 -1.3 2,358,187 + PP_2072 0.85 +0.7 2,358,188 - PP_2072 0.85 +1.0 2,358,188 - +2.8 2,358,262 + -0.3 2,358,263 - -1.6 2,358,418 + PP_2073 0.17 +1.0 2,358,418 + PP_2073 0.17 -2.1 2,358,609 + PP_2073 0.54 -2.7 2,358,790 + PP_2073 0.89 -0.8 2,358,791 - PP_2073 0.89 -0.9 2,358,936 - +0.0 2,358,957 - -0.2 2,359,265 + PP_2074 0.24 +0.8 2,359,266 - PP_2074 0.24 -0.5 2,359,454 + PP_2074 0.44 +0.6 2,359,501 + PP_2074 0.50 +0.4 2,359,548 + PP_2074 0.55 -0.4 2,359,549 - PP_2074 0.55 +0.1 2,359,549 - PP_2074 0.55 +1.6 2,359,570 - +1.5 2,359,785 - PP_2074 0.80 +2.2
Or see this region's nucleotide sequence