Strain Fitness in Pseudomonas putida KT2440 around PP_2034

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,310,806	+		PP_2033	0.42	+0.2
2,310,806	+		PP_2033	0.42	-0.1
2,310,807	-		PP_2033	0.42	+0.7
2,310,918	+		PP_2033	0.51	+0.1
2,310,919	-		PP_2033	0.51	+0.5
2,310,975	+		PP_2033	0.55	+0.5
2,311,050	-		PP_2033	0.61	-0.9
2,311,076	+		PP_2033	0.63	+0.0
2,311,077	-		PP_2033	0.63	+1.8
2,311,119	+		PP_2033	0.66	-1.6
2,311,201	+		PP_2033	0.73	-0.0
2,311,202	-		PP_2033	0.73	+1.3
2,311,202	-		PP_2033	0.73	-2.4
2,311,202	-		PP_2033	0.73	-0.5
2,311,202	-		PP_2033	0.73	-1.8
2,311,213	+		PP_2033	0.73	-1.4
2,311,214	-		PP_2033	0.74	-0.1
2,311,253	-		PP_2033	0.77	+0.8
2,311,438	+				-0.6
2,311,438	+				-0.8
2,311,438	+				-1.5
2,311,438	+				+0.4
2,311,439	-				-0.5
2,311,642	+				-1.6
2,311,642	+				-0.0
2,311,642	+				-0.3
2,311,643	-				-0.9
2,311,643	-				+0.8
2,312,110	-		PP_2034	0.41	-2.1
2,312,225	+		PP_2034	0.50	-0.5
2,312,226	-		PP_2034	0.50	-0.6
2,312,226	-		PP_2034	0.50	+1.5
2,312,274	+		PP_2034	0.54	-1.0
2,312,275	-		PP_2034	0.54	-1.7
2,312,365	-		PP_2034	0.61	-1.2
2,312,405	+		PP_2034	0.64	+1.1
2,312,405	+		PP_2034	0.64	-1.5
2,312,405	+		PP_2034	0.64	+2.4
2,312,405	+		PP_2034	0.64	-0.1
2,312,405	+		PP_2034	0.64	-1.2
2,312,405	+		PP_2034	0.64	-1.4
2,312,405	+		PP_2034	0.64	-2.3
2,312,405	+		PP_2034	0.64	+0.6
2,312,406	-		PP_2034	0.64	+0.3
2,312,406	-		PP_2034	0.64	-2.0
2,312,406	-		PP_2034	0.64	+0.2
2,312,406	-		PP_2034	0.64	+1.4
2,312,406	-		PP_2034	0.64	-1.0
2,312,406	-		PP_2034	0.64	-1.8
2,312,406	-		PP_2034	0.64	-0.6
2,312,406	-		PP_2034	0.64	-1.2
2,312,406	-		PP_2034	0.64	-0.6
2,312,409	+		PP_2034	0.65	-0.7
2,312,409	+		PP_2034	0.65	-1.4
2,312,409	+		PP_2034	0.65	-1.7
2,312,409	+		PP_2034	0.65	-0.8
2,312,409	+		PP_2034	0.65	+0.1
2,312,409	+		PP_2034	0.65	+0.7
2,312,409	+		PP_2034	0.65	+0.1
2,312,409	+		PP_2034	0.65	+2.2
2,312,410	-		PP_2034	0.65	-1.2
2,312,410	-		PP_2034	0.65	-0.6
2,312,410	-		PP_2034	0.65	+0.6
2,312,410	-		PP_2034	0.65	-0.4
2,312,410	-		PP_2034	0.65	-0.4
2,312,410	-		PP_2034	0.65	+1.3
2,312,410	-		PP_2034	0.65	-0.1
2,312,412	-		PP_2034	0.65	-0.3
2,312,412	-		PP_2034	0.65	-0.5
2,312,479	+		PP_2034	0.70	-0.7
2,312,479	+		PP_2034	0.70	-0.4
2,312,479	+		PP_2034	0.70	-1.7
2,312,480	-		PP_2034	0.70	-0.5
2,312,480	-		PP_2034	0.70	-2.4
2,312,550	+		PP_2034	0.76	+0.4
2,312,609	-		PP_2034	0.80	-2.0
2,312,623	+		PP_2034	0.82	+0.2
2,312,677	-		PP_2034	0.86	+1.3
2,312,688	+		PP_2034	0.87	-1.0
2,312,819	+				-2.1
2,313,101	-		PP_2035	0.19	+1.0
2,313,165	+		PP_2035	0.24	-0.3
2,313,165	+		PP_2035	0.24	-0.9
2,313,166	-		PP_2035	0.24	+1.0
2,313,166	-		PP_2035	0.24	+0.3
2,313,166	-		PP_2035	0.24	+0.4
2,313,166	-		PP_2035	0.24	-1.1
2,313,166	-		PP_2035	0.24	-0.9
2,313,378	+		PP_2035	0.42	-0.5
2,313,378	+		PP_2035	0.42	+0.5
2,313,378	+		PP_2035	0.42	-0.8
2,313,497	+		PP_2035	0.51	+0.8
2,313,498	-		PP_2035	0.51	-0.9
2,313,520	-		PP_2035	0.53	-0.1
2,313,520	-		PP_2035	0.53	-1.7
2,313,585	+		PP_2035	0.59	-2.4
2,313,585	+		PP_2035	0.59	-0.5
2,313,585	+		PP_2035	0.59	-0.8
2,313,585	+		PP_2035	0.59	-0.5
2,313,586	-		PP_2035	0.59	+0.5
2,313,586	-		PP_2035	0.59	-0.5
2,313,586	-		PP_2035	0.59	-1.2
2,313,586	-		PP_2035	0.59	+0.2
2,313,701	+		PP_2035	0.68	-0.8
2,313,769	-		PP_2035	0.74	-0.1
2,313,776	+		PP_2035	0.74	-0.8
2,313,776	+		PP_2035	0.74	+0.2
2,313,777	-		PP_2035	0.74	+0.6
2,313,790	-				+0.8
2,313,790	-		PP_2035	0.75	+1.4